Journal ArticleDOI
Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function.
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TLDR
It is indicated that the survival and/or proliferation of the differentiating type A spermatogonia requires c-kit, but the primitive (undifferentiated) type C sperMatogonia or sPermatogenic stem cells are independent from c-Kit.Abstract:
Recent studies have shown that the dominant white spotting (W) locus encodes the proto-oncogene c-kit, a member of the tyrosine kinase receptor family. One symptom of mice bearing mutation within this gene is sterility due to developmental failure of the primordial germ cells during early embryogenesis. To elucidate the role of the c-kit in gametogenesis, we used an anti-c-kit monoclonal antibody, ACK2, as an antagonistic blocker for c-kit function to interfere with the development of male and female germ cells during postnatal life. ACK2 enabled us to detect the expression of c-kit in the gonadal tissue and also to determine the functional status of c-kit, which is expressed on the surface of a particular cell lineage. Consistent with our immunohistochemical findings, the intravenous injection of ACK2 into adult mice caused a depletion in the differentiating type A spermatogonia from the testis during 24-36 h, while the undifferentiated type A spermatogonia were basically unaffected. Intraperitoneal injections of ACK2 into prepuberal mice could completely block the mitosis of mature (differentiating) type A spermatogonia, but not the mitosis of the gonocytes and primitive type A spermatogonia, or the meiosis of spermatocytes. Our results indicate that the survival and/or proliferation of the differentiating type A spermatogonia requires c-kit, but the primitive (undifferentiated) type A spermatogonia or spermatogenic stem cells are independent from c-kit. Moreover, the antibody administration had no significant effect on oocyte maturation despite its intense expression of c-kit.read more
Citations
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Journal ArticleDOI
Regulation of cell fate decision of undifferentiated spermatogonia by GDNF.
Xiaojuan Meng,Maria Lindahl,Mervi E. Hyvönen,Martti Parvinen,Dirk G. de Rooij,Michael W. Hess,Anne Raatikainen-Ahokas,Kirsi Sainio,Heikki Rauvala,Merja Lakso,José G. Pichel,Heiner Westphal,Mart Saarma,Hannu Sariola +13 more
TL;DR: Transgenic loss-of-function and overexpression models show that the dosage of glial cell line-derived neurotrophic factor (GDNF), produced by Sertoli cells, regulates cell fate decisions of undifferentiated sperMatogonial cells that include the stem cells for spermatogenesis.
Journal ArticleDOI
Stem Cell Factor and Hematopoiesis
TL;DR: Stem cell factor (SCF), also known as kit ligand, mast cell growth factor, or steel factor, is a hematopoietic cytokine that promotes the survival, proliferation, and differentiation of stem cells and progenitor cells.
Journal ArticleDOI
All you wanted to know about spermatogonia but were afraid to ask.
Journal ArticleDOI
miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis
Wei Deng,Haifan Lin +1 more
TL;DR: The cloning of a murine piwi gene (miwi) essential for spermatogenesis is reported, and MIWI complexes with mRNAs of ACT and CREM target genes are reported, suggesting that MIWI may control spermiogenesis by regulating the stability of these m RNAs.
Journal ArticleDOI
Dominant role of the niche in melanocyte stem-cell fate determination
Emi K. Nishimura,Emi K. Nishimura,Siobhán A. Jordan,Hideo Oshima,Hideo Oshima,Hisahiro Yoshida,Masatake Osawa,Mariko Moriyama,Ian J. Jackson,Yann Barrandon,Yoshiki Miyachi,Shin-Ichi Nishikawa +11 more
TL;DR: It is reported that stem cells of the melanocyte lineage can be identified, using Dct-lacZ transgenic mice, in the lower permanent portion of mouse hair follicles throughout the hair cycle and indicated that the niche has a dominant role in the fate determination of melanocyte stem-cell progeny.
References
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Journal ArticleDOI
Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand.
Yosef Yarden,Wun-Jing Kuang,T. Yang-Feng,Lisa M. Coussens,S. Munemitsu,Thomas J. Dull,Ellson Y. Chen,Joseph Schlessinger,Uta Francke,Axel Ullrich +9 more
TL;DR: The results suggest that p145c‐kit functions as a cell surface receptor for an as yet unidentified ligand, and carboxy‐ and amino‐terminal truncations that occurred during the viral transduction process are likely to have generated the transformation potential of v‐kit.
Journal ArticleDOI
Stem cell factor is encoded at the SI locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor
Krisztina M. Zsebo,David A. Williams,Edwin N. Geissler,Virginia C. Broudy,Francis Hall Martin,Harry L. Atkins,Rou Yin Hsu,Neal C. Birkett,Kenneth H. Okino,Murdock Douglas C,Frederick W. Jacobsen,Keith Langley,Kent A. Smith,Takashi Takeish,Bruce M. Cattanach,Stephen J. Galli,Sidney Vaughn Suggs +16 more
TL;DR: In vivo administration of SCF can reverse the macrocytic anemia and locally repair the mast cell deficiency of Sl/Sld mice and provide biological and physical evidence that SCF is a ligand for the c-kit receptor.
Journal ArticleDOI
The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus.
TL;DR: Observations provide the first example of a germ-line mutation in a mammalian proto-oncogene and implicate the c-kit gene as a candidate for the W locus and provide a molecular entry into this important region of the mouse genome.
Journal ArticleDOI
Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization.
TL;DR: The successful isolation of these prepuberal cell types was accomplished by defining distinctive morphological characteristics of the cells and assessing the identity and purity of the isolated cell types by microscopy.
Journal ArticleDOI
The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene.
TL;DR: It is shown here that the c-kit gene is disrupted in two spontaneous mutant W alleles, W44 and Wx, which strongly support the identification of c-Kit as the gene product of the W locus.