Journal ArticleDOI
Shotgun lipidomics: Electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples
Xianlin Han,Richard W. Gross +1 more
TLDR
This review will focus on the advances in ESI/MS, which have facilitated the development of shotgun lipidomics and the utility of intrasource separation as an enabling strategy for utilization of 2D mass spectrometry in Shotgun lipidomics of biological samples.Abstract:
Lipidomics, after genomics and proteomics, is a newly and rapidly expanding research field that studies cellular lipidomes and the organizational hierarchy of lipid and protein constituents mediating life processes. Lipidomics is greatly facilitated by recent advances in, and novel applications of, electrospray ionization mass spectrometry (ESI/MS). In this review, we will focus on the advances in ESI/MS, which have facilitated the development of shotgun lipidomics and the utility of intrasource separation as an enabling strategy for utilization of 2D mass spectrometry in shotgun lipidomics of biological samples. The principles and experimental details of the intrasource separation approach will be extensively discussed. Other ESI/MS approaches towards the quantitative analyses of global cellular lipidomes directly from crude lipid extracts of biological samples will also be reviewed and compared. Multiple examples of lipidomic analyses from crude lipid extracts employing these approaches will be given to show the power of ESI/MS techniques in lipidomics. Currently, modern society is plagued by the sequelae of lipid-related diseases. It is our hope that the integration of these advances in multiple disciplines will catalyze the development of lipidomics, and such development will lead to improvements in diagnostics and therapeutics, which will ultimately result in the extended longevity and an improved quality of life for humankind.read more
Citations
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Journal ArticleDOI
Mass spectrometry-based metabolomics.
TL;DR: This review presents an overview of the dynamically developing field of mass spectrometry-based metabolomics, a technique that analyzes all detectable analytes in a given sample with subsequent classification of samples and identification of differentially expressed metabolites, which define the sample classes.
Journal ArticleDOI
Innovation: Metabolomics: the apogee of the omics trilogy
TL;DR: By performing global metabolite profiling, also known as untargeted metabolomics, new discoveries linking cellular pathways to biological mechanism are being revealed and are shaping the understanding of cell biology, physiology and medicine.
Journal ArticleDOI
The Human Serum Metabolome
Nikolaos Psychogios,David Hau,Jun Peng,An Chi Guo,Rupasri Mandal,Souhaila Bouatra,Igor Sinelnikov,Ramanarayan Krishnamurthy,Roman Eisner,Bijaya Gautam,Nelson Young,Jianguo Xia,Craig Knox,Edison Dong,Paul Huang,Zsuzsanna Hollander,Theresa L. Pedersen,Steven R. Smith,Fiona Bamforth,Russell Greiner,Bruce M. McManus,John W. Newman,Theodore L. Goodfriend,David S. Wishart,David S. Wishart +24 more
TL;DR: This work has combined targeted and non-targeted NMR, GC-MS and LC-MS methods with computer-aided literature mining to identify and quantify a comprehensive, if not absolutely complete, set of metabolites commonly detected and quantified (with today's technology) in the human serum metabolome.
Journal ArticleDOI
Lipidomics: coming to grips with lipid diversity
Andrej Shevchenko,Kai Simons +1 more
TL;DR: It is argued that lipidomics will become an essential tool kit in cell and developmental biology, molecular medicine and nutrition and the quantitative characterization of the lipidome.
Journal ArticleDOI
LipidBlast in silico tandem mass spectrometry database for lipid identification
Tobias Kind,Kwang-Hyeon Liu,Kwang-Hyeon Liu,Do Yup Lee,Do Yup Lee,Brian C. DeFelice,John K. Meissen,Oliver Fiehn +7 more
TL;DR: This work provides a freely available computer-generated tandem mass spectral library of 212,516 spectra covering 119,200 compounds from 26 lipid compound classes, including phospholipids, glycerolipid, bacterial lipoglycans and plant glycolipids.
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Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: A bridge to lipidomics
Xianlin Han,Richard W. Gross +1 more