Stepwise and dynamic assembly of the earliest precursors of small ribosomal subunits in yeast
TLDR
The constituents of yeast 90S particles that were assembled on a series of plasmid-encoded 3'-truncated pre-18S RNAs are affinity-purified and analyzed, revealing a stepwise and dynamic assembly map that provides a new framework to understand small subunit biogenesis.Abstract:
The eukaryotic ribosomal RNA (rRNA) is associated cotranscriptionally with numerous factors into an enormous 90S preribosomal particle that conducts early processing of small ribosomal subunits. The assembly pathway and structure of the 90S particle is poorly understood. Here, we affinity-purified and analyzed the constituents of yeast 90S particles that were assembled on a series of plasmid-encoded 3'-truncated pre-18S RNAs. We determined the assembly point of 65 proteins and the U3, U14, and snR30 small nucleolar RNAs (snoRNAs), revealing a stepwise and dynamic assembly map. The 5' external transcribed spacer (ETS) alone can nucleate a large complex. When the 18S rRNA is nearly complete, the 90S structure undergoes a dramatic reorganization, releasing U14, snR30, and 14 protein factors that bind earlier. We also identified a reference state of 90S that is fully assembled yet has not undergone 5'ETS processing. The assembly map present here provides a new framework to understand small subunit biogenesis.read more
Citations
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Journal ArticleDOI
Ribosome assembly coming into focus.
TL;DR: These studies highlight the roles of RNA compaction, checkpoints and proofreading mechanisms of pre-ribosomal particles in the nucleolus, nucleus and cytoplasm of yeast ribosome assembly intermediates.
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Eukaryotic ribosome assembly, transport and quality control.
TL;DR: Mechanisms by which the ribosome assembly and nucleocytoplasmic transport machineries collaborate to produce functional ribosomes are discussed.
Journal ArticleDOI
Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.
Markus Kornprobst,Martin Turk,Nikola Kellner,Jingdong Cheng,Dirk Flemming,Isabelle C Kos-Braun,Martin Kos,Matthias Thoms,Otto Berninghausen,Roland Beckmann,Ed Hurt +10 more
TL;DR: The cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome is reported, revealing how a network of biogenesis factors including 19 β-propellers and large α-solenoid proteins engulfs the pre-rRNA.
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A Puzzle of Life: Crafting Ribosomal Subunits
TL;DR: This review integrates first structural insights into an updated overview of the consecutive ribosome assembly steps with the recent visualization of several assembly intermediates by cryoelectron microscopy (cryo-EM).
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Chromatin-associated RNAs as facilitators of functional genomic interactions
Xiao Li,Xiang-Dong Fu +1 more
TL;DR: Diverse roles are being revealed for these RNAs and associated RNA-binding proteins (RBPs) in gene regulation and genome function, which include locus-specific roles in gene activation and silencing, as well as emerging roles in higher-order genome organization.
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