Stepwise histone replacement by SWR1 requires dual activation with histone H2A.Z and canonical nucleosome.
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TLDR
It is shown that promoter-proximal nucleosomes are highly heterogeneous for H2A.Z in Saccharomyces cerevisiae, with substantial representation of nucleosome containing one, two, or zero H2a.Z molecules.About:
This article is published in Cell.The article was published on 2010-11-24 and is currently open access. It has received 402 citations till now. The article focuses on the topics: Histone methylation & Histone code.read more
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Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals
Upasna Sharma,Colin C. Conine,Jeremy M. Shea,Ana Bošković,Alan G. Derr,Xin Y. Bing,Clémence Belleannée,Alper Kucukural,Ryan W. Serra,Fengyun Sun,Lina Song,Benjamin R. Carone,Emiliano P. Ricci,Xin Zhiguo Li,Lucas Fauquier,Melissa J. Moore,Robert Sullivan,Craig C. Mello,Manuel Garber,Oliver J. Rando +19 more
TL;DR: The results shed light on sRNA biogenesis and its dietary regulation during posttesticular sperm maturation, and they also link tRNA fragments to regulation of endogenous retroelements active in the preimplantation embryo.
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Histone exchange, chromatin structure and the regulation of transcription
TL;DR: Ongoing research is elucidating the molecular mechanisms that regulate chromatin structure during transcription by preventing histone exchange, thereby limiting non-coding RNA expression.
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Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes
TL;DR: The 'hourglass' model of remodeller function is proposed, in which each remodeller subfamily utilizes diverse specialized proteins and protein domains to assist in nucleosomes targeting or to differentially detect nucleosome epitopes.
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New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?
TL;DR: The compaction of genomic DNA into chromatin has profound implications for the regulation of key processes such as transcription, replication and DNA repair, and it is becoming clear that chromatin structures are not nearly as uniform and regular as previously assumed.
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DNA methylation dynamics during epigenetic reprogramming in the germline and preimplantation embryos
TL;DR: Recent technological advances in genome-wide DNA methylation analysis and the functional description of novel enzymatic DNA demethylation pathways have provided significant insights into the molecular processes that prepare the mammalian embryo for normal development.
References
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Twenty-Five Years of the Nucleosome, Fundamental Particle of the Eukaryote Chromosome
Roger D. Kornberg,Yahli Lorch +1 more
TL;DR: The chromatin field needs much more information about structure beyond the nucleosome, and there is insufficient evidence that acetylation actually causes chromatin unfolding, and functional analysis in cell-free systems must be extended beyond theucleosome to the chromosomal context.
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A New Efficient Gene Disruption Cassette for Repeated Use in Budding Yeast
TL;DR: A loxP-kanMX-loxP gene disruption cassette is developed, which combines the advantages of the heterologous kanr marker with those from the Cre- lox P recombination system, and will be of great advantage for the functional analysis of gene families.
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New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
P.T Lowary,Julia R. Widom +1 more
TL;DR: In this paper, a set of highest affinity molecules were selected, cloned and sequenced, their affinities (free energies) for histone octamer in nucleosome reconstitution measured, and their ability to position nucleosomes in vitro assessed by native gel electrophoresis.
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ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex
TL;DR: It is found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with hist one H2AZ in nucleosome arrays.
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Nucleosome positioning and gene regulation: advances through genomics
Cizhong Jiang,B. Franklin Pugh +1 more
TL;DR: What high-resolution genome-wide maps of nucleosomes positions have taught us about how nucleosome positioning demarcates promoter regions and transcriptional start sites and how the composition and structure of promoter nucleosites facilitate or inhibit transcription is discussed.