Journal ArticleDOI
Structure of the yellow fever NS5 protein reveals conserved drug targets shared among flaviviruses.
Anna Dubankova,Evzen Boura +1 more
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TLDR
The structure of the full-length NS5 protein, a key enzyme for the replication of flaviviruses that contains both a methyltransferase domain and an RNA dependent RNA polymerase domain, is reported at 3.1 Å resolution and suggests NS5 as a prime and ideal target for the design of pan-flavivirus inhibitors.About:
This article is published in Antiviral Research.The article was published on 2019-09-01. It has received 40 citations till now. The article focuses on the topics: Flavivirus & Dengue fever.read more
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Structural analysis of the SARS-CoV-2 methyltransferase complex involved in RNA cap creation bound to sinefungin.
TL;DR: The authors provide the structure of nsp10-nsp16 with the panMTase inhibitor sinefungin and report that the development of MTase inhibitor therapies that target multiple coronoaviruses is feasible.
Journal ArticleDOI
Structural analysis of the putative SARS-CoV-2 primase complex.
TL;DR: Analysis of the surface electrostatic potential revealed a putative RNA binding site that is formed only within the heterotetramer of the SARS-CoV-2 putative primase composed of the nsp7 and nsp8 proteins.
Journal ArticleDOI
Remdesivir triphosphate can efficiently inhibit the RNA-dependent RNA polymerase from various flaviviruses.
Eva Konkolova,Milan Dejmek,Hubert Hřebabecký,Michal Šála,Jiří Böserle,Radim Nencka,Evzen Boura +6 more
TL;DR: The results show that remdesivir can efficiently inhibit RdRps from viruses causing severe illnesses such as Yellow fever, West Nile fever, Japanese and Tick-borne encephalitis, Zika and Dengue.
Journal ArticleDOI
Zinc and Copper Ions Differentially Regulate Prion-Like Phase Separation Dynamics of Pan-Virus Nucleocapsid Biomolecular Condensates.
TL;DR: The analyses reveal that metal-coordinated virus NC protein PrLDs initiate LLPS that nucleate pan-virus assembly and contribute to their persistence as cell-free infectious aerosol droplets, representing an additional virus strategy impeding physiological and cellular antiviral responses.
Journal ArticleDOI
Evaluation of sofosbuvir activity and resistance profile against West Nile virus in vitro.
Filippo Dragoni,Adele Boccuto,Francesca Picarazzi,Alessia Giannini,Federica Giammarino,Francesco Saladini,Mattia Mori,Eloise Mastrangelo,Maurizio Zazzi,Ilaria Vicenti +9 more
TL;DR: To the authors' knowledge, this is the first report of the antiviral activity of sofosbuvir against WNV as well as of selection of mutants in vitro.
References
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A Mechanism for Initiating RNA-Dependent RNA Polymerization
TL;DR: The results provide a working model for the initiation of replication and transcription of double-stranded RNA viruses and flaviviruses and suggest a mechanism by which the incoming double-Stranded RNA is opened up to feed the template through to the active site, while the substrates enter by another route.
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Crystal Structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface
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