Journal ArticleDOI
Surface-modified superparamagnetic nanoparticles for drug delivery: preparation, characterization, and cytotoxicity studies
Ajay Kumar Gupta,S. Wells +1 more
TLDR
The cytotoxicity profile of the nanoparticles on human dermal fibroblasts as measured by standard 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay showed that the particles are nontoxic and may be useful for various in vivo and in vitro biomedical applications.Abstract:
Superparamagnetic iron oxide nanoparticles have been used for many years as magnetic resonance imaging (MRI) contrast agents or in drug delivery applications. In this study, a novel approach to prepare magnetic polymeric nanoparticles with magnetic core and polymeric shell using inverse microemulsion polymerization process is reported. Poly(ethyleneglycol) (PEG)-modified superparamagnetic iron oxide nanoparticles with specific shape and size have been prepared inside the aqueous cores of AOT/n-Hexane reverse micelles and characterized by various physicochemical means such as transmission electron microscopy (TEM), infrared spectroscopy, atomic force microscopy (AFM), vibrating sample magnetometry (VSM), and ultraviolet/visible spectroscopy. The inverse microemulsion polymerization of a polymerizable derivative of PEG and a cross-linking agent resulted in a stable hydrophilic polymeric shell of the nanoparticles. The results taken together from TEM and AFM studies showed that the particles are spherical in shape with core-shell structure. The average size of the PEG-modified nanoparticles was found to be around 40-50 nm with narrow size distribution. The magnetic measurement studies revealed the superparamagnetic behavior of the nanoparticles with saturation magnetization values between 45-50 electromagnetic units per gram. The cytotoxicity profile of the nanoparticles on human dermal fibroblasts as measured by standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the particles are nontoxic and may be useful for various in vivo and in vitro biomedical applications.read more
Citations
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Journal ArticleDOI
Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications
Ajay Kumar Gupta,Mona Gupta +1 more
TL;DR: This review discusses the synthetic chemistry, fluid stabilization and surface modification of superparamagnetic iron oxide nanoparticles, as well as their use for above biomedical applications.
Journal ArticleDOI
Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and Biological Applications
Sophie Laurent,Delphine Forge,Marc Port,Alain Roch,Caroline Robic,Luce Vander Elst,Robert N. Muller +6 more
TL;DR: Practical Interests of Magnetic NuclearRelaxation for the Characterization of Superparamagnetic Colloid, and Use of Nanoparticles as Contrast Agents forMRI20825.
Book ChapterDOI
Applications of magnetic nanoparticles in biomedicine
TL;DR: The physical principles underlying some current biomedical applications of magnetic nanoparticles are reviewed and the relevant physics of magnetic materials and their responses to applied magnetic fields are surveyed.
Journal ArticleDOI
Cytotoxicity of Nanoparticles
TL;DR: For nanoparticles to move into the clinical arena, it is important that nanotoxicology research uncovers and understands how these multiple factors influence the toxicity of nanoparticles so that their undesirable properties can be avoided.
Journal ArticleDOI
Magnetic Iron Oxide Nanoparticles: Synthesis and Surface Functionalization Strategies
TL;DR: The problems and major challenges, along with the directions for the synthesis and surface functionalization of iron oxide NPs, are considered and some future trends and prospective in these research areas are also discussed.
References
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis.
TL;DR: The magnitude of the cytotoxic effects of all polymers were found to be time- and concentration dependent and the molecular weight as well as the cationic charge density of the polycations were confirmed as key parameters for the interaction with the cell membranes and consequently the cell damage.
Journal ArticleDOI
Ultrafine metal particles
TL;DR: In this paper, a statistical growth model based on the Central Limit Theorem has been formulated for liquid-like coalescence of particles; this theory accounts satisfactorily for all the data, as well as for most size distributions published in the literature.
Journal ArticleDOI
Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake.
TL;DR: PEG and folic acid modification of magnetite nanoparticles could be used to resist the protein adsorption and thus avoid the particle recognition by macrophage cells, and to facilitate the nanoparticle uptake to specific cancer cells for cancer therapy and diagnosis.
Journal ArticleDOI
Surface modification of nanoparticles to oppose uptake by the mononuclear phagocyte system
TL;DR: The literature reviewed provides enough promise for anticipating therapeutic and diagnostic applications of surface-modified nanoparticles, with particular focus on the literature concerning particles other than liposomes.
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Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications
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