Journal ArticleDOI
Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.
TLDR
Recommendations for probe design and hybridization are presented, and the choice between using multiple probes reflecting all codon possibilities and a unique optimal probe is discussed.About:
This article is published in Journal of Molecular Biology.The article was published on 1985-05-05. It has received 844 citations till now. The article focuses on the topics: Multiplex ligation-dependent probe amplification & Genetic code.read more
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Patent
Amyloid precursor-like protein and uses thereof
TL;DR: In this paper, the authors presented a method to isolate amyloid precursor-like proteins (APLPs), nucleotide sequences coding for and regulating expression of these protein, antibodies directed against these proteins, and recombinant vectors and host cells containing the genetic sequences of these proteins.
Journal ArticleDOI
A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members.
John A. Thompson,S. Mössinger,V. Reichardt,U. Engels,Nicole Beauchemin,F. Kommoss,S. von Kleist,Wolfgang Zimmermann +7 more
TL;DR: Two sets of oligonucleotide primers were developed for the specific amplification of transcripts from individual CEA‐family members using the reverse transcriptase/ polymerase chain reaction (RT/PCR) to find co‐expression of CEA and NCA transcripts, and occasionally BGP mRNA.
Journal ArticleDOI
A candidate marsupial PrP gene reveals two domains conserved in mammalian PrP proteins.
TL;DR: The deduced marsupial PrP has an overall identity of about 80% to eutherian PrP at the amino acid (aa) level, but this similarity is not equally distributed and two regions are particularly highly conserved.
Journal ArticleDOI
Single host and symbiont lineages of hydrothermal-vent gastropods Ifremeria nautilei (Provannidae) : biogeography and evolution
Yohey Suzuki,Shigeaki Kojima,Hiromi Watanabe,Masae Suzuki,Shinji Tsuchida,Takuro Nunoura,Hisako Hirayama,Ken Takai,Kenneth H. Nealson,Koki Horikoshi +9 more
TL;DR: Phylogenetic relationships inferred among gastropod hosts and among their endo- symbionts were not congruent, implying that acquisition of endosymbionts might be from the environment rather than through vertical transmission.
Patent
Vista regulatory T cell mediator protein, vista binding agents and use thereof
Randolph J. Noelle,Lili Wang +1 more
TL;DR: The PD-L3 OR VISTA (V-domain Immunoglobulin Suppressor of T cell Activation) as discussed by the authors was proposed to suppress T cell immunity in autoimmune disease, allergy, infection and inflammatory conditions.
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Journal ArticleDOI
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells
Axel Ullrich,Lisa M. Coussens,Joel S. Hayflick,Thomas J. Dull,Alane M. Gray,A W Tam,Jeffrey E. Lee,Yosef Yarden,Towia A. Libermann,Joseph Schlessinger +9 more
TL;DR: The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains.
Journal ArticleDOI
Improved Estimation of Secondary Structure in Ribonucleic Acids
Ignacio Tinoco,Philip N. Borer,Barbara Dengler,Mark D. Levin,Olke C. Uhlenbeck,Donald M. Crothers,J. Bralla +6 more
TL;DR: This method can be used for predicting and assessing possible secondary structures for recently determined RNA sequences and new experimental and theoretical results allow us to improve the method, without making it more complicated.
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Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Journal ArticleDOI
DNA methylation and the frequency of CpG in animal DNA.
TL;DR: An analysis of nearest neighbour dinucleotide frequencies and the level of DNA methylation in animals strongly supports the suggestion that 5-methylcytosine (5mC) tends to mutate abnormally frequently to T.
Journal ArticleDOI
Codon catalog usage is a genome strategy modulated for gene expressivity
TL;DR: The nucleic acid sequence bank now contains 161 mRNAs, 43 new genes are added, and internal regulation of mRNA expression by different third base choices between quartet and duet codons is proposed for bacterial genes.