Journal ArticleDOI
T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV
David Klatzmann,Eric Champagne,S. Chamaret,Jacqueline Gruest,Denise Guetard,Thierry Hercend,Jean Claude Gluckman,Luc Montagnier +7 more
TLDR
Preincubation of T4+ lymphocytes with three individual monoclonal antibodies directed at the T4 glycoprotein blocked cell infection by LAV, strongly support the view that a surface molecule directly involved in cellular functions acts as, or is related to, the receptor for a human retrovirus.Abstract:
Many viruses, including retroviruses, are characterized by their specific cell tropism. Lymphadenopathy-associated virus (LAV) is a human lymphotropic retrovirus isolated from patients with acquired immune deficiency syndrome (AIDS) or related syndromes, that displays selective tropism for a subset of T lymphocytes defined by the expression of a surface glycoprotein of relative molecular mass 62,000 (62K) termed T4 (refs 6-8). This glycoprotein delineates a subset of T lymphocytes with mainly helper/inducer functions, while T lymphocytes of the reciprocal subset express a glycoprotein termed T8, have mainly cytotoxic/suppressor activities, and are unable to replicate LAV. Such a tropism may be controlled at the genomic level by regulatory sequences, as described for the human T-cell leukaemia viruses HTLV-I and -II (refs 2, 3). Alternatively or concomitantly, productive cell infection may be controlled at the membrane level, requiring the interaction of a specific cellular receptor with the virus envelope, as demonstrated recently for Epstein-Barr virus (EBV). Therefore, we have investigated whether the T4 molecule itself is related to the receptor for LAV. We report here that preincubation of T4+ lymphocytes with three individual monoclonal antibodies directed at the T4 glycoprotein blocked cell infection by LAV. This blocking effect was specific, as other monoclonal antibodies--such as antibody to histocompatibility locus antigen (HLA) class II or anti-T-cell natural killer (TNK) target--directed at other surface structures strongly expressed on activated cultured T4+ cells, did not prevent LAV infection. Direct virus neutralization by monoclonal antibodies was also ruled out. These results strongly support the view that a surface molecule directly involved in cellular functions acts as, or is related to, the receptor for a human retrovirus.read more
Citations
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Journal ArticleDOI
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus
Angus Dalgleish,Peter C. L. Beverley,Paul R. Clapham,Dorothy H. Crawford,Mel Greaves,Robin A. Weiss +5 more
TL;DR: It is concluded that the CD4 antigen is an essential and specific component of the receptor for the causative agent of AIDS.
Journal ArticleDOI
Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
Akio Adachi,Howard E. Gendelman,Scott Koenig,Thomas M. Folks,Ronald Willey,Arnold B. Rabson,M A Martin +6 more
TL;DR: In this paper, an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus was constructed, and upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells.
Journal ArticleDOI
The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Primary HIV-1 Isolates
Hyeryun Choe,Michael Farzan,Ying Sun,Nancy Sullivan,Barrett J. Rollins,Paul D. Ponath,Lijun Wu,Charles R. Mackay,Gregory J. LaRosa,Walter Newman,Norma P. Gerard,Craig Gerard,Joseph Sodroski +12 more
TL;DR: The ability of various members of the chemokine receptor family to support the early stages of HIV-1 infection helps to explain viral tropism and beta-chemokine inhibition of primary HIV- 1 isolates.
Journal ArticleDOI
The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.
Paul J. Maddon,Angus G. Dalgleish,J. Steven McDougal,Paul R. Clapham,Robin A. Weiss,Richard Axel +5 more
TL;DR: The studies support a mechanism of AIDS virus infection that initially involves the specific interaction of theAIDS virus with T4 molecules on the cell surface, and find that the T4 gene is expressed in the brain as well as in lymphoid cells, providing an explanation for the dual neurotropic and lymphotropic character of the AIDS virus.
Journal ArticleDOI
The HIV-1 envelope glycoproteins: fusogens, antigens, and immunogens.
Richard T. Wyatt,Joseph Sodroski +1 more
TL;DR: The human immunodeficiency virus-type 1 (HIV-1) envelope glycoproteins interact with receptors on the target cell and mediate virus entry by fusing the viral and cell membranes.
References
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Journal ArticleDOI
Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).
Françoise Barré-Sinoussi,J. C. Chermann,Félix A. Rey,Marie-Thérèse Nugeyre,S. Chamaret,Jacqueline Gruest,Charles Dauguet,Claudine Axler-Blin,F. Vézinet-Brun,Christine Rouzioux,Willy Rozenbaum,Luc Montagnier +11 more
TL;DR: From these studies it is concluded that this virus as well as the previous HTLV isolates belong to a general family of T-lymphotropic retroviruses that are horizontally transmitted in humans and may be involved in several pathological syndromes, including AIDS.
Journal ArticleDOI
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus
Angus Dalgleish,Peter C. L. Beverley,Paul R. Clapham,Dorothy H. Crawford,Mel Greaves,Robin A. Weiss +5 more
TL;DR: It is concluded that the CD4 antigen is an essential and specific component of the receptor for the causative agent of AIDS.
Journal ArticleDOI
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS
Robert C. Gallo,Syed Zaki Salahuddin,Mikulas Popovic,Gene M. Shearer,Mark H. Kaplan,Barton F. Haynes,Thomas J. Palker,Robert R. Redfield,James M. Oleske,Bijan Safai,Gilbert C. White,Paul Foster,Phillip D. Markham +12 more
TL;DR: Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell growth factor and assayed for the expression and release of human T-lymphotropic retroviruses (HTLV).
Journal ArticleDOI
The differentiation and function of human T lymphocytes
TL;DR: The precise dissection of cellular mechanisms and interactions involved in the generation of human T cell responses has been facilitated in recent years by advances in four areas: first, the development of in this paper.
Journal ArticleDOI
Separation of functional subsets of human T cells by a monoclonal antibody.
TL;DR: OKT4 could be a valuable reagent for determining alterations of these functional subsets in human diseases and suggest that the OKT4+ subset represents a helper population and that the OkT4- subset contains the cytotoxic effector population.