The four ribosomal DNA units of the malaria parasite Plasmodium berghei. Identification, restriction map, and copy number analysis.
J B Dame,T F McCutchan +1 more
TLDR
The four ribosomal RNA genes of the rodent malaria parasite were identified and mapped by restriction enzyme analysis and Southern blot hybridization of genomic DNA and restriction analysis confirms both the low copy number and that these genes are not in an easily recognizable tandem array.About:Â
This article is published in Journal of Biological Chemistry.The article was published on 1983-06-10 and is currently open access. It has received 84 citations till now. The article focuses on the topics: Ribosomal DNA & Restriction map.read more
Citations
More filters
Journal ArticleDOI
Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii
Jane M. Carlton,Samuel V. Angiuoli,Bernard B. Suh,Taco W. A. Kooij,Mihaela Pertea,Joana C. Silva,Maria D. Ermolaeva,Jonathan E. Allen,Jeremy D. Selengut,Hean L. Koo,Jeremy Peterson,Mihai Pop,Daniel S. Kosack,Martin Shumway,Shelby L. Bidwell,Shamira J. Shallom,Susan Van Aken,Steven B. Riedmuller,Tamara Feldblyum,Jennifer Cho,John Quackenbush,Martha Sedegah,Azadeh Shoaibi,Leda M. Cummings,Laurence Florens,John R. Yates,J. Dale Raine,Robert E. Sinden,Michael Harris,Deirdre A. Cunningham,Peter R. Preiser,Lawrence W. Bergman,Akhil B. Vaidya,Leo H.M. van Lin,Chris J. Janse,Andrew P. Waters,Hamilton O. Smith,Owen White,Steven L. Salzberg,J. Craig Venter,Claire M. Fraser,Stephen L. Hoffman,Malcolm J. Gardner,Daniel J. Carucci +43 more
TL;DR: This is the first genome sequence of a model eukaryotic parasite, and it provides insight into the use of such systems in the modelling of Plasmodium biology and disease.
Journal ArticleDOI
Structurally distinct, stage-specific ribosomes occur in Plasmodium
John H. Gunderson,Mitchell L. Sogin,G Wollett,M R Hollingdale,VF de la Cruz,Andrew P. Waters,Thomas F. McCutchan +6 more
TL;DR: Two structurally distinct nuclear genes code for cytoplasmic small subunit ribosomal RNA's in the parasite Plasmodium berghei, and when the parasite infects the mammalian host, transcripts from the second gene become the predominant small subunits of the parasite species.
Journal ArticleDOI
Evolutionary Relatedness of Plasmodium Species as Determined by the Structure of DNA
TL;DR: The evolutionary lines suggested here appear to be consistent with similarities seen among malaria parasites with regard to gene sequence.
Journal ArticleDOI
Primary sequences of two small subunit ribosomal RNA genes from Plasmodium falciparum.
Thomas F. McCutchan,Vidal F. de la Cruz,Altaf A. Lal,John H. Gunderson,Hille J. Elwood,Mitchell L. Sogin +5 more
TL;DR: From comparisons of the Plasmodium genes to that of humans, it was possible to design genus-specific as well as species-specific oligonucleotide probes that can be used to distinguish the parasite 18S ribosomal RNA from that of its host.
Journal ArticleDOI
Detection and quantitation of Anaplasma marginale in carrier cattle by using a nucleic acid probe.
TL;DR: A nucleic acid probe is developed, derived from a portion of a gene encoding a 105,000-molecular-weight surface protein, that can detect A. marginale-infected erythrocytes and shows that parasitemia levels were highly variable among carriers, implying that, at any given time, individuals within a group of cattle may differ significantly in their abilities to transmit disease.
References
More filters
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco BolĂvar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
The isolation of structural genes from libraries of eucaryotic DNA
Tom Maniatis,Ross C. Hardison,Elizabeth Lacy,Joyce Lauer,Catherine O'Connell,Diana Quon,Gek Kee Sim,Argiris Efstratiadis +7 more
TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.
Journal ArticleDOI
Repeated genes in eukaryotes.
Eric O. Long,Igor B. Dawid +1 more
TL;DR: A large number of the ribosomal genes affected by the bobbed locus mutation in Drosophila belong to the TSP class, which has been associated with central giant cell reprograming.