The properties of a mammary gland cancer stem cell
Ileana Zucchi,S. Sanzone,Simonetta Astigiano,Paride Pelucchi,Maddalena Scotti,V. Valsecchi,Ottavia Barbieri,Gloria Bertoli,Alberto Albertini,Rolland Reinbold,Renato Dulbecco +10 more
TLDR
The properties of self-renewal, extensive capacity for proliferation, multilineage differentiation potential, and single-cell tumor-initiation potential suggest that LA7 cells are cancer stem cells and can be used as a model system to study the dynamics of tumor formation at the single- cell level.Abstract:
The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing a single cancer-initiating cell with stem cell properties has proven technically difficult because of the scarcity of the cancer stem cells in the tissue of origin and the lack of specific markers for cancer stem cells. Here we show that a single LA7 cell derived from rat mammary adenocarcinoma has the following properties: the differentiation potential to generate all of the cell lineages of the mammary gland; the ability to generate branched duct-like structures that recapitulate morphologically and functionally the ductal–alveolar-like architecture of the mammary tree; and the capacity to initiate heterogeneous tumors in nonobese diabetic-SCID mice. In addition, we show that cultured cells derived from tumors generated by a single LA7 cell-injection have properties similar to LA7 cells, can generate all of the cell lineages of the mammary gland, and recapitulate the ductal–alveolar-like architecture of the mammary tree. The properties of self-renewal, extensive capacity for proliferation, multilineage differentiation potential, and single-cell tumor-initiation potential suggest that LA7 cells are cancer stem cells and can be used as a model system to study the dynamics of tumor formation at the single-cell level.read more
Citations
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CD44 is of Functional Importance for Colorectal Cancer Stem Cells
Lei Du,Hongyi Wang,Leya He,Jingyu Zhang,Biyun Ni,Xiaohui Wang,Haijing Jin,Nathalie Cahuzac,Maryam Mehrpour,Youyong Lu,Quan Chen +10 more
TL;DR: CD44 is a robust marker and is of functional importance for colorectal CSC for cancer initiation and Knockdown of CD44, but not CD133, strongly prevented clonal formation and inhibited tumorigenicity in xenograft model.
Journal ArticleDOI
Microenvironmental regulation of stem cells in intestinal homeostasis and cancer
Jan Paul Medema,Louis Vermeulen +1 more
TL;DR: The microenvironment-derived signals that regulate stem-cell fate and epithelial differentiation are highlighted and may point towards new insights into the origin of cancer and the maintenance and regulation of cancer stem cells.
Journal ArticleDOI
Cancer stem cell markers in common cancers – therapeutic implications
Thomas Klonisch,Emilia Wiechec,Sabine Hombach-Klonisch,Sudharsana R. Ande,Sebastian Wesselborg,Klaus Schulze-Osthoff,Marek Los +6 more
TL;DR: In this article, the specific markers that have been found to be present on stem cells, cancer cells and CSCs in selected tissues (colon, breast, liver, pancreas and prostate) are compared.
Journal ArticleDOI
The developing cancer stem-cell model: clinical challenges and opportunities
TL;DR: The cancer stem cells (CSCs) were identified as the clonogenic core of the tumor and therefore represent the cell population that drives growth and progression as mentioned in this paper, however, recent insights have complicated the initial elegant model, by showing a dominant role for the tumour microenvironment in determining CSC characteristics within a malignancy.
Journal ArticleDOI
Combined targeted treatment to eliminate tumorigenic cancer stem cells in human pancreatic cancer.
Maria Theresa Mueller,Patrick C. Hermann,Patrick C. Hermann,Juliane Witthauer,Belen Rubio-Viqueira,Simon F. Leicht,Stephan Huber,Joachim W. Ellwart,Mona Mustafa,Peter Bartenstein,Jan G. D’Haese,Michael H. Schoenberg,Frank Berger,Karl-Walter Jauch,Manuel Hidalgo,Christopher Heeschen,Christopher Heeschen +16 more
TL;DR: The combined blockade of sonic hedgehog and mTOR signaling together with standard chemotherapy is capable of eliminating pancreatic CSCs.
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