Journal ArticleDOI
The real-time polymerase chain reaction
Mikael Kubista,José Manuel Andrade,Martin Bengtsson,Amin Forootan,Jiri Jonák,Kristina Lind,Radek Sindelka,Robert Sjöback,Björn Sjögreen,Linda Strömbom,Anders Ståhlberg,Neven Zoric +11 more
TLDR
The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR, a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the mid 80:ies.About:
This article is published in Molecular Aspects of Medicine.The article was published on 2006-04-01. It has received 1383 citations till now. The article focuses on the topics: Polymerase chain reaction.read more
Citations
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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Stephen A. Bustin,Vladimir Benes,Jeremy A. Garson,Jan Hellemans,Jim F. Huggett,Mikael Kubista,Reinhold Mueller,Tania Nolan,Michael W. Pfaffl,Gregory L. Shipley,Jo Vandesompele,Carl T. Wittwer,Carl T. Wittwer +12 more
TL;DR: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency.
Journal ArticleDOI
Assessment of Ki67 in Breast Cancer: Recommendations from the International Ki67 in Breast Cancer Working Group
Mitch Dowsett,Torsten O. Nielsen,Roger A'Hern,John M. S. Bartlett,R. Charles Coombes,Jack Cuzick,Matthew J. Ellis,N. Lynn Henry,Judith Hugh,Tracy Lively,Lisa M. McShane,Soon Paik,Frédérique Penault-Llorca,Ljudmila Prudkin,Meredith M. Regan,Janine Salter,Christos Sotiriou,Ian E. Smith,Giuseppe Viale,Jo Anne Zujewski,Daniel F. Hayes +20 more
TL;DR: Comprehensive recommendations on preanalytical and analytical assessment, and interpretation and scoring of Ki67 were formulated based on current evidence, geared toward achieving a harmonized methodology, create greater between-laboratory and between-study comparability, and allow earlier valid applications of this marker in clinical practice.
Journal ArticleDOI
Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TL;DR: The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.
Journal ArticleDOI
PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
TL;DR: A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size.
Journal ArticleDOI
Gold Nanoparticles for In Vitro Diagnostics
TL;DR: This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the response of the immune system to EMTs.
References
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Journal ArticleDOI
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Jo Vandesompele,Katleen De Preter,Filip Pattyn,Bruce Poppe,Nadine Van Roy,Anne De Paepe,Franki Speleman +6 more
TL;DR: The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which opens up the possibility of studying the biological relevance of small expression differences.
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Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
TL;DR: Development and application of REST is explained, the usefulness of relative expression in real-time PCR using REST is discussed and the mathematical model used is based on the PCR efficiencies and the mean crossing point deviation between the sample and control group.
Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal ArticleDOI
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.
TL;DR: The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index, which can be compared with further target genes to decide, whether they are differentially expressed under an applied treatment.
Journal ArticleDOI
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.
TL;DR: The technical aspects involved are discussed, conventional and kinetic RT-PCR methods for quantitating gene expression are contrasted, and the usefulness of these assays are illustrated by demonstrating the significantly different levels of transcription between individuals of the housekeeping gene family, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH).