Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Protein Content of Model Synovial Fluid and CoCrMo Wear
H. Stevenson,Philippa Cann +1 more
TL;DR: Tests indicate the chemistry of human synovial fluid, particularly the protein content, could affect CoCrMo wear and therefore the risk of implant failure and the formation of gel deposits is comparable to the “boosted” lubrication model of proposed by Professor Duncan Dowson for articular cartilage.
Journal ArticleDOI
Two Roads Converged in a Yellow Dye: Unusual Spectral Broadening in the Emission of Auramine-O Possibly Caused by Low-Friction Intramolecular Rotation
TL;DR: In this article, steady state and time-resolved optical techniques were employed to study the rather complex relaxation of excited states of Auramine-O (AuO) in several liquids at room temperature.
Journal ArticleDOI
Glyoxal Induced Transition of Transferrin to Aggregates: Spectroscopic, Microscopic and Molecular Docking Insight
TL;DR: It is proposed that glyoxal induces the formation of aggregates in hTF which generate ROS which are key players in the complications associated to diabetes mellitus, giving the study clinical perspective.
Journal ArticleDOI
The role of charge transfer in the photophysics of dithiophene-based (NIADs) fluorescent markers for amyloid-β detection
TL;DR: Modifications on the NIAD-4 skeleton have little effects on the ground and excited state properties of the dye as well as on the feasibility of the most probable non-radiative deactivation pathways, but affect significantly the blood–brain barrier (BBB) permeability and binding site preference toward Aβ fibrils.
Journal ArticleDOI
Effect of pH on the structure and function of cyclin-dependent kinase 6
Mohd Yousuf,Anas Shamsi,Farah Anjum,Alaa Shafie,Asimul Islam,Qazi Mohammad Rizwanul Haque,Abdelbaset Mohamed Elasbali,Dharmendra Kumar Yadav,Md. Imtaiyaz Hassan +8 more
TL;DR: Investigation of the structural and conformational changes in CDK6 at varying pH employing a multi-spectroscopic approach revealed that the tertiary structure ofCDK6 was disrupted under extremely acidic conditions, with slight alteration occurring in mild acidic conditions.
References
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Journal ArticleDOI
General atomic and molecular electronic structure system
Michael W. Schmidt,Kim K. Baldridge,Jerry A. Boatz,Steven T. Elbert,Mark S. Gordon,Jan H. Jensen,Shiro Koseki,Nikita Matsunaga,Kiet A. Nguyen,Shujun Su,Theresa L. Windus,Michel Dupuis,John A. Montgomery +12 more
TL;DR: A description of the ab initio quantum chemistry package GAMESS, which can be treated with wave functions ranging from the simplest closed‐shell case up to a general MCSCF case, permitting calculations at the necessary level of sophistication.
BookDOI
Molecular fluorescence : principles and applications
TL;DR: In this article, the effects of intermolecular photophysical processes on fluorescence emission are discussed and an analysis of the effect of polarity of fluorescence emissions is presented.
Journal ArticleDOI
Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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