Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status
Minna Groenning,Minna Groenning +1 more
TL;DR: Increased understanding of present molecular probes as well as development of new probes are of utmost importance for development of strategies to control amyloid formation and overcome neurodegenerative disorders.
Journal ArticleDOI
Thioflavin T as an Efficient Inducer and Selective Fluorescent Sensor for the Human Telomeric G-Quadruplex DNA
Jyotirmayee Mohanty,Nilotpal Barooah,V. Dhamodharan,S. Harikrishna,Pushpangadan Indira Pradeepkumar,Achikanath C. Bhasikuttan +5 more
TL;DR: The application of a water-soluble fluorogenic dye, Thioflavin T (ThT), in a dual role of exclusively inducing quadruplex folding in the 22AG human telomeric DNA, both in the presence and absence of Tris buffer/salt, is demonstrated.
Journal ArticleDOI
Molecular rotors: what lies behind the high sensitivity of the thioflavin-T fluorescent marker.
TL;DR: The advantages and disadvantages of the various nonradiative models while focusing on the model that was initially proposed by Glasbeek and co-workers for auramine-O to be the best suited for ThT are discussed.
Journal ArticleDOI
Protein-induced photophysical changes to the amyloid indicator dye thioflavin T
Leslie S. Wolfe,Matthew F. Calabrese,Abhinav Nath,Dorottya Blaho,Andrew D. Miranker,Yong Xiong +5 more
TL;DR: These efforts fundamentally extend existing understanding about the origins of amyloid-induced photophysical changes by preventing the fluorophore from adopting its preferred excited state configuration, nonradiative relaxation pathways are minimized and quantum yield is increased.
Journal ArticleDOI
ThT 101: a primer on the use of thioflavin T to investigate amyloid formation
Kirsten Gade Malmos,Luis M. Blancas-Mejia,Benedikt Weber,Johannes Buchner,Marina Ramirez-Alvarado,Hironobu Naiki,Daniel E. Otzen +6 more
TL;DR: A conceptual instruction manual, covering appropriate considerations and pitfalls related to the use of ThT, and recommendations for complementary techniques to address shortcomings in the ThT assay.
References
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Journal ArticleDOI
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Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status
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