Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Chirality and Chiroptical Properties of Amyloid Fibrils
TL;DR: The current state of research on amyloid-like fibrils is outlined from the perspective of their structural and superstructural chirality and chiroptical properties.
Journal ArticleDOI
Thioflavin T behaves as an efficient fluorescent ligand for label-free ATP aptasensor
TL;DR: Thioflavin T (ThT) is demonstrated as an efficient fluorescent ligand for 27-mer ATP-binding aptamer (ABA27), providing a novel signal readout mode for label-free selective ATP detection and may make ThT able to serve as a facile signal reporter for DNA nanomechanical devices fueled with ATP.
Journal ArticleDOI
ThX – a next-generation probe for the early detection of amyloid aggregates
Lisa-Maria Needham,Judith Weber,Juan A. Varela,James W. B. Fyfe,Dung T. Do,Catherine K. Xu,Luke Tutton,Rachel Cliffe,Benjamin Keenlyside,David Klenerman,Christopher M. Dobson,Christopher A. Hunter,Karin H. Müller,Kevin O'Holleran,Sarah E. Bohndiek,Thomas N. Snaddon,Steven F. Lee +16 more
TL;DR: Thioflavin X, (ThX), a next-generation fluorescent probe which displays superior properties; including a 5-fold increase in brightness and 7-fold increased in binding affinity to amyloidogenic proteins, is designed.
Posted ContentDOI
Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
Peter A. Summers,Ben Lewis,Jorge González-García,Aaron H. M. Lim,Paolo Cadinu,Rosa Maria Porreca,Nerea Martin-Pintado,David J. Mann,Joshua B. Edel,Jean-Baptiste Vannier,Marina K. Kuimova,Ramon Vilar +11 more
TL;DR: It is demonstrated that a fluorescent probe (DAOTA-M2) in conjunction with Fluorescence Lifetime Imaging Microscopy (FLIM) can identify G4 within nuclei of live and fixed cells and suggests an increased number of G4 in these cells, implying that FancJ and RTEL1 play a role in resolving G4 structures in cellulo.
Journal ArticleDOI
Synthesis and Aggregation Studies of a Pyridothiazole-BasedAIEE Probe and Its Application in Sensing Amyloid Fibrillation
Nidhi Gour,Vivekshinh Kshtriya,Shradhey Gupta,Bharti Koshti,Ramesh Singh,Dhaval Patel,Khashti Ballabh Joshi +6 more
TL;DR: In this paper, the aggregation and photophysical properties of a pyridothiazole-based, aggregation-induced, emissionenhancement (AIEE) luminogen 4-(5methoxy-thiazolo[4,5-b]pyridin-2-yl)benzoic acid (P...
References
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Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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