Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Kinetics of Melanin Polymerization during Enzymatic and Nonenzymatic Oxidation.
TL;DR: Models made with density functional theory show that the linear homopolymeric structures of 5,6-dihydroxyindole can account for experimentally observed wavenumbers and broad bandwidth in Raman spectra of dopa-melanin.
Journal ArticleDOI
Dual effect of non-ionic detergent Triton X-100 on insulin amyloid formation.
TL;DR: Analysis of the experimental and molecular modeling results suggests an existence of up to six TX-100 binding sites within dimer of insulin with different binding energy, and the physiological relevance of the results is discussed.
Journal ArticleDOI
Dimethyl Sulfoxide Induced Destabilization and Disassembly of Various Structural Variants of Insulin Fibrils Monitored by Vibrational Circular Dichroism
TL;DR: VCD is utilized to characterize changes in the macroscopic chirality following DMSO-induced disassembly for two types of insulin fibrils formed under different conditions, at different reduced pH values with and without added salt and agitation, and it is confirmed that very high concentrations of D MSO can disaggregate both types.
Journal ArticleDOI
Time Resolved Photoelectron Spectroscopy of Thioflavin T Photoisomerization: A Simulation Study
TL;DR: Simulations reveal that the variation of the twisting angle between benzothiazole and benzene groups (φ1) is responsible for the subpicosecond fluorescence quenching of thioflavin T in a nonrestricted environment.
Journal ArticleDOI
Thioflavin T derivatives for the characterization of insulin and lysozyme amyloid fibrils in vitro: Fluorescence and quantum-chemical studies
TL;DR: In this paper, two charged Thioflavin T derivatives, referred to here as ICT2 and ICT3, showed higher fluorescence response, association constants and the blue-shifted emission maxima in the presence of lysozyme fibrils compared to insulin aggregates.
References
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General atomic and molecular electronic structure system
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Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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