Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Surface patterns of insulin fibrils revealed by time-resolved spectroscopy measurements of fluorescent probes
TL;DR: In this paper, the surface patterns of insulin fibrils and its polymorphs seeded with LVEALYL peptide were detected using Thioflavin T (ThT) and polythiophene derivative poly[2-(3-thienyl)ethoxy-4-butylsulfonate] (PTEBS) polymer.
Journal ArticleDOI
Modulatory role of copper on hIAPP aggregation and toxicity in presence of insulin.
Dipanwita Roy,Narayan C. Maity,Sourav Kumar,Anupam Maity,Bhisma N Ratha,Ranjit Biswas,Nakul C. Maiti,Atin K. Mandal,Anirban Bhunia +8 more
TL;DR: In this article , the authors used a set of biophysical techniques, including NMR, fluorescence, CD etc., in combination with AFM and cell cytotoxicity assay.
Journal ArticleDOI
Mechanistic insight into functionally different human islet polypeptide (hIAPP) amyloid: the intrinsic role of the C-terminal structural motifs.
D. Sarkar,Narayan C. Maity,Gourav Shome,Kyriakos G. Varnava,Vijayalekshmi Sarojini,Subramanian Vivekanandan,Nirakar Sahoo,Sourav Kumar,Atin K. Mandal,Ranjit Biswas,Anirban Bhunia +10 more
TL;DR: A study involving an array of biophysical experiments and ex vivo data suggests a direct influence of external changes on the C-terminal fibrillating sequence, which indicates a possible collaborative role of this segment in nucleating hIAPP amyloidogenesis in a physiological scenario, thus making it a potential target for future therapeutic interventions.
Journal ArticleDOI
Exploring the Effect of Aliphatic Substituents on Aryl Cyano Amides on Enhancement of Fluorescence upon Binding to Amyloid-β Aggregates.
Rachel S Ehrlich,Alexander L Shiao,Meihan Li,Kristine L Teppang,Kun Yong Jeoung,Emmanuel A. Theodorakis,Jerry Yang +6 more
TL;DR: In this paper, the authors investigated the effect of small aliphatic substituents on the spectroscopic properties of ARCAM both free in solution and when bound to aggregated Aβ.
References
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TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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