Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Monitoring amyloid aggregation via a twisted intramolecular charge transfer (TICT)-based fluorescent sensor array
Chao Wang,Wenchao Jiang,Davin Tan,Lu Huang,Jin Li,Qing Long Qiao,Priya Yadav,Xiaogang Liu,Zhaochao Xu +8 more
TL;DR: In this article , a platter of hemicyanines with varied sensitivities and dynamic ranges is synthesized, forming a sensor array and enabling the observation of various stages of Alzheimer's disease (AD) aggregation.
Journal ArticleDOI
Probing the Tl+-induced Structural Transformation of G-rich DNA to G-quadruplex with a Label-free Thioflavin T-based Biosensor
TL;DR: In this article , the Tl+-induced structural change (Free State → G4) was probed with the Thioflavin T (ThT), a typical label-free G4 dye.
Journal ArticleDOI
Cavity hairpin ThT-light nucleic acid switches: the construction of label- and enzyme-free sensing and imaging platforms
Xinyue Lan,Longjiao Zhu,Yangzi Zhang,Keren Chen,Jia Wang,Zaihui Du,Shuting Li,Xu Chen,Wentao Xu +8 more
TL;DR: Wang et al. as discussed by the authors proposed CGG-AAA mismatched cavity hairpin Thioflavin T-light nucleic acid switches (CHTLNAS) with excellent fluorescence excitation over 500-fold higher than spontaneous, 17∼20-fold high than ssDNA and 2.5∼5-fold more than complementary duplex, which achieved the label and enzyme-free detection of TET and berberine at the pM level within 10 min.
Journal ArticleDOI
Extracting conformational information from single molecule photon statistics.
TL;DR: The approach of resonant trajectories of photon emission in the conformational coordinate X and external field frequency ωL space to extract conformational information of single molecule is described.
Journal ArticleDOI
Effect of Substituents on TICT Rate in Thioflavin T-Based Fluorescent Molecular Rotors
Vitali I. Stsiapura,S. D. Gogoleva,A. A. Maskevich,O. V. Buganov,Sergei A. Tikhomirov,A. A. Lugovski,Kalpita Baruah,Bani Kanta Sarma +7 more
TL;DR: In this article, a modification of the Thioflavin T (ThT) structure via introduction of methyl and methoxy groups was described, and the effects of the substituents on fluorescence were investigated.
References
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Journal ArticleDOI
General atomic and molecular electronic structure system
Michael W. Schmidt,Kim K. Baldridge,Jerry A. Boatz,Steven T. Elbert,Mark S. Gordon,Jan H. Jensen,Shiro Koseki,Nikita Matsunaga,Kiet A. Nguyen,Shujun Su,Theresa L. Windus,Michel Dupuis,John A. Montgomery +12 more
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Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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