Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
In situ viscosity measurement of confined liquids
TL;DR: In this paper, the fluorescence lifetime of a fluorescent dye that is sensitive to viscosity was quantified using an in-vivo measurement technique for high pressure, high shear conditions.
Journal ArticleDOI
Pressure Effect on the Nonradiative Process of Thioflavin-T
TL;DR: A remarkable linear correlation between a decrease in k(nr) (increase in the nonradiative lifetime, τ(nr)) and an increase in the solvent viscosity, which is explained by the excited-state rotation rate of the two-ring systems, with respect to each other.
Journal ArticleDOI
High Fluorescence Anisotropy of Thioflavin T in Aqueous Solution Resulting from Its Molecular Rotor Nature.
Irina M. Kuznetsova,Anna I. Sulatskaya,A. A. Maskevich,Vladimir N. Uversky,Vladimir N. Uversky,Konstantin K. Turoverov,Konstantin K. Turoverov +6 more
TL;DR: By steady-state and femtosecond time-resolved fluorescence it is shown that, unlike small sized rigid molecules, the fluorescence anisotropy value of the free ThT in aqueous solutions is very high, close to the limiting value.
Journal ArticleDOI
Novel Green Procedure for the Synthesis of 2-Arylbenzothiazoles Under Microwave Irradiation in Peg 200 Or Peg 400
TL;DR: An improved green procedure for the synthesis of 2-aryl and 2-hetarylbenzothiazoles by condensation of equivalent amounts of 2,2′-diaminodiphenyldisulfides or 2-aminothiophenols and various aromatic aldehydes in PEG 200/400 under microwave irradiation has been developed.
Journal ArticleDOI
Ultrafast Twisting Dynamics of Thioflavin-T: Spectroscopy of the Twisted Intramolecular Charge-Transfer State
Rajib Ghosh,Dipak K. Palit +1 more
TL;DR: The spectroscopic properties of the intramolecular charge-transfer state are characterized for the first time and a longer-lived transient state populated with a very low yield has been characterized as the triplet state.
References
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Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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