Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Evidence for pheomelanin sheet structure
TL;DR: In this article, the authors present evidence from fluorescence spectroscopy for pheomelanin also displaying sheet-like behavior, and show that the temporal response of the fluorescence intensity of the sheet-sensing probe thioflavin T (ThT) follows a similar sigmoidal increase as previously reported for eumelin.
Journal ArticleDOI
Cholinergic inhibitors replace thioflavin-T from acetylcholinesterase binding pocket: A potential fluorescence based molecular switch
TL;DR: The fluorescence intensity of acetylcholinesterase bound thioflavin-T (ThT) is found to decrease regularly in presence of cholinergic inhibitors, which can be correlated with AChE inhibition activity of the drugs.
Journal ArticleDOI
Paper-Based Distance Sensor for the Detection of Lipase via a Phase Separation-Induced Viscosity Change.
Shuang Xia,Fangchao Yin,Lulu Xu,Binglu Zhao,Wenli Wu,Ya Feng Ma,Jin-Ming Lin,Yulin Liu,Mei Zhao,Qiongzheng Hu +9 more
TL;DR: In this paper , a paper-based lipase sensor was proposed to detect acute pancreatitis via a phase separation-induced viscosity change, which can be quantified by measuring the solution flow distance on a pH test paper.
Journal ArticleDOI
Amyloid fibril formation by pepsin in neutral pH at room temperature
TL;DR: Fibril formation was characterized by a number of techniques including fluorescence and circular dichroism spectroscopy, dynamic light scattering, and transmission electron microscopy.
References
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Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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