Journal ArticleDOI
Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity.
Vitali I. Stsiapura,A. A. Maskevich,V. A. Kuzmitsky,Vladimir N. Uversky,Irina M. Kuznetsova,Konstantin K. Turoverov +5 more
TLDR
The photophysical model successfully explains the fluorescent properties of ThT in solvents with different viscosities and supports the idea that the reason for the characteristic increase in the ThT fluorescence intensity accompanying its incorporation into the amyloid fibrils is determined by the rigidity of the dye environment.Abstract:
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms of the characteristic increase in ThT fluorescence intensity accompanying its incorporation into the amyloid-like fibrils. To this end, the dependencies of the ThT quantum yield and fluorescence lifetime on temperature and glycerol content in the water−glycerol mixtures are studied. It has been found that fluorescent properties of ThT are typical for the specific class of fluorophores known as molecular rotors. It has been established that the low ThT fluorescence intensity in the solvents with low viscosity is caused by the nonradiative deactivation of the excited state associated with the torsional motion of the ThT benzthiazole and aminobenzene rings relative to each other, which results in the transition of ThT molecule to nonfluorescent twisted internal charge transfer (TICT) state. The rate of this process is determined by the solvent viscosity, whereas the emission does oc...read more
Citations
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Journal ArticleDOI
Selective recognition of ds-DNA cavities by a molecular rotor: switched fluorescence of thioflavin T
TL;DR: It is reported that the fluorescence intensity of ThT is obviously enhancement in when it binds to ds-DNAs which contain cavity structures such as an abasic site, gap site or mismatch site, and it is expected that the ability to target these DNA structures has the potential to be developed into practical and functional biomaterials for DNA sensors or devices.
Journal ArticleDOI
Molecular rotors as luminescence sensors of local viscosity and viscous flow in solutions and organized systems
TL;DR: In this paper, the results of studies of various classes of molecular rotors are generalized, and the structures of the main classes of rotors and principles of their functioning are analyzed.
Journal ArticleDOI
Color modeling of protein optical probes.
TL;DR: This work addresses the structural and optical properties of nile red within the β-lacto globulin protein and finds the absorption properties of the probe to be highly dependent on its position relative to the protein.
Journal ArticleDOI
Characterization of Aqueous Oleic Acid/Oleate Dispersions by Fluorescent Probes and Raman Spectroscopy
TL;DR: The results suggest that large-scale OA/oleate assemblies could possess particular membrane properties in a narrow pH region, e.g., at pH 7.5, and 9.7.
Journal ArticleDOI
Inhibition of Amyloid-β Aggregation by Cobalt(III) Schiff Base Complexes: A Computational and Experimental Approach.
Aysenur Iscen,Christopher R. Brue,Kaleigh F. Roberts,Joy Kim,George C. Schatz,Thomas J. Meade +5 more
TL;DR: This study reports the synthesis and characterization of a novel cobalt(III) Schiff base complex with methylamine axial ligands, and presents both computational and experimental data demonstrating the reduction of β-sheet formation by this complex.
References
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Thioflavine T interaction with synthetic Alzheimer's disease beta-amyloid peptides: detection of amyloid aggregation in solution.
TL;DR: Thioflavine T associates rapidly with aggregated fibrils of the synthetic β/A4‐derived peptides β( 1–28) and β(1–40), giving rise to a new excitation maximum at 450 nm and enhanced emission at 482 nm, as opposed to the 385 nm and 445 nm of the free dye.
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