Two Mammalian Longevity Assurance Gene (LAG1) Family Members, trh1 and trh4, Regulate Dihydroceramide Synthesis Using Different Fatty Acyl-CoA Donors
Reads0
Chats0
TLDR
Two additional members of this family regulate the synthesis of (dihydro)ceramides with specific fatty acid(s) when overexpressed in human embryonic kidney 293T cells, lending support to the hypothesis (Venkataraman, K., and Futerman, A. H. 528, 3–4) that Lag1p family members regulate ( dihydra)ceramide synthases responsible for production of sphingolipids containing different fatty acids.About:
This article is published in Journal of Biological Chemistry.The article was published on 2003-10-31 and is currently open access. It has received 285 citations till now. The article focuses on the topics: Ceramide synthase & Ceramide.read more
Citations
More filters
Journal ArticleDOI
Inhibition of Ceramide Synthesis Ameliorates Glucocorticoid-, Saturated-Fat-, and Obesity-Induced Insulin Resistance
William L. Holland,Joseph T. Brozinick,Liping Wang,Eric D. Hawkins,Katherine M. Sargent,Yanqi Liu,Krishna K. Narra,Kyle L. Hoehn,Trina A. Knotts,Angela M. Siesky,Don H. Nelson,Sotirios K. Karathanasis,Greg K Fontenot,Morris J. Birnbaum,Scott A. Summers +14 more
TL;DR: It is demonstrated that the sphingolipid ceramide is a common molecular intermediate linking several different pathological metabolic stresses to the induction of insulin resistance, and enzymes required for ceramide synthesis are identified as therapeutic targets for combating insulin resistance caused by nutrient excess or glucocorticoid therapy.
Book ChapterDOI
An overview of sphingolipid metabolism: from synthesis to breakdown.
TL;DR: This chapter will pay tribute to the complex regulation of simple sphingolipids, a class of lipids defined by their eighteen carbon amino-alcohol backbones that play significant roles in membrane biology and provide many bioactive metabolites that regulate cell function.
Journal ArticleDOI
The complex life of simple sphingolipids
TL;DR: This work outlines exciting progress in the biochemistry and cell biology of sphingolipids and focuses on their functional diversity, which should set the conceptual and experimental framework that will eventually lead to a fully integrated and comprehensive model of the functions of specific sphingosine‐1‐phosphate in regulating defined aspects of cell physiology.
Journal ArticleDOI
SphK1 and SphK2, Sphingosine Kinase Isoenzymes with Opposing Functions in Sphingolipid Metabolism
Michael Maceyka,Heidi Sankala,Nitai C. Hait,Hervé Le Stunff,Hong Liu,Rachelle E. Toman,Claiborne Lee Collier,Min Zhang,Leslie S. Satin,Alfred H. Merrill,Sheldon Milstien,Sarah Spiegel +11 more
TL;DR: The results demonstrate that SphK1 and SphK2 have opposing roles in the regulation of ceramide biosynthesis and suggest that the location of sphingosine 1-phosphate production dictates its functions.
Journal ArticleDOI
Sphingolipid and Glycosphingolipid Metabolic Pathways in the Era of Sphingolipidomics
TL;DR: A major goal of the review is to help the reader more easily grasp the metabolic interrelationships that account for the tens of thousands of molecular subspecies that appear in nature, with a focus on mammals.
References
More filters
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Programmed cell death induced by ceramide
TL;DR: The effects of C2-ceramide on DNA fragmentation were prevented by the protein kinase C activator phorbol 12-myristate 13-acetate, which suggests the existence of two opposing intracellular pathways in the regulation of apoptosis.
Journal ArticleDOI
Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor
TL;DR: High molecular weight DNA from cells containing only one copy of the HSV gene coding for tk was successfully used to transform L+K-cells to the tk+ phenotype, and the acquired phenotype was demonstrated to be donor-derived by analysis of the electrophoretic mobility of the tK activity.