Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles
Matthew Kirkham,Akikazu Fujita,Rahul Chadda,Susan J. Nixon,Teymuras V. Kurzchalia,Deepak Sharma,Richard E. Pagano,John F. Hancock,Satyajit Mayor,Robert G. Parton +9 more
Reads0
Chats0
TLDR
Using quantitative light microscopy and a modified immunoelectron microscopic technique, the entry pathway of the cholera toxin binding subunit (CTB) in primary embryonic fibroblasts is characterized and the major carriers involved in initial entry of CTB were identified as uncoated tubular or ring-shaped structures.Abstract:
Using quantitative light microscopy and a modified immunoelectron microscopic technique, we have characterized the entry pathway of the cholera toxin binding subunit (CTB) in primary embryonic fibroblasts CTB trafficking to the Golgi complex was identical in caveolin-1null (Cav1-/-) mouse embryonic fibroblasts (MEFs) and wild-type (WT) MEFs CTB entry in the Cav1-/- MEFs was predominantly clathrin and dynamin independent but relatively cholesterol dependent Immunoelectron microscopy was used to quantify budded and surface-connected caveolae and to identify noncaveolar endocytic vehicles In WT MEFs, a small fraction of the total Cav1-positive structures were shown to bud from the plasma membrane (2% per minute), and budding increased upon okadaic acid or lactosyl ceramide treatment However, the major carriers involved in initial entry of CTB were identified as uncoated tubular or ring-shaped structures These carriers contained GPI-anchored proteins and fluid phase markers and represented the major vehicles mediating CTB uptake in both WT and caveolae-null cellsread more
Citations
More filters
Journal ArticleDOI
Mechanisms of Endocytosis
TL;DR: What is known about mammalian endocytic mechanisms is reviewed, with focus on the cellular proteins that control these events, and the functional relevance of distinctendocytic pathways is discussed.
Journal ArticleDOI
Cellular uptake of nanoparticles: journey inside the cell.
Shahed Behzadi,Vahid Serpooshan,Wei Tao,Majd A. Hamaly,Mahmoud Y. Alkawareek,Erik C. Dreaden,Dennis Brown,Alaaldin M. Alkilany,Omid C. Farokhzad,Omid C. Farokhzad,Morteza Mahmoudi,Morteza Mahmoudi +11 more
TL;DR: This review provides an objective and comprehensive account of the cellular uptake of NPs and the underlying parameters controlling the nano-cellular interactions, along with the available analytical techniques to follow and track these processes.
Journal ArticleDOI
Pathways of clathrin-independent endocytosis.
Satyajit Mayor,Richard E. Pagano +1 more
TL;DR: The current understanding of various clathrin-independent mechanisms of endocytosis are reviewed and a classification scheme is proposed to help organize the data in this complex and evolving field.
Journal ArticleDOI
The multiple faces of caveolae
Robert G. Parton,Kai Simons +1 more
TL;DR: The apparently diverse functions of caveolae, including mechanosensing and lipid regulation, might be linked to their ability to respond to plasma membrane changes, a property that is dependent on their specialized lipid composition and biophysical properties.
Journal ArticleDOI
Virus entry: open sesame.
Mark Marsh,Ari Helenius +1 more
TL;DR: Cell biology studies, live-cell imaging, and systems biology have started to illuminate the multiple and subtly different pathways that animal viruses use to enter host cells, revolutionizing the understanding of endocytosis and the movement of vesicles within cells.
References
More filters
Journal ArticleDOI
Regulated portals of entry into the cell
Sean D. Conner,Sandra L. Schmid +1 more
TL;DR: ‘Endocytosis’ encompasses several diverse mechanisms by which cells internalize macromolecules and particles into transport vesicles derived from the plasma membrane and must be viewed in a broader context than simple vesicular trafficking.
Journal ArticleDOI
Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER.
TL;DR: The results demonstrate the existence of a two-step transport pathway from plasma-membrane caveolae, through an intermediate organelle (termed the caveosome), to the ER, which bypasses endosomes and the Golgi complex, and is part of the productive infectious route used by SV40.
Journal ArticleDOI
Induction of mutant dynamin specifically blocks endocytic coated vesicle formation.
TL;DR: It is concluded that dynamin is specifically required for endocytic coated vesicle formation, and that its GTP binding and hydrolysis activities are required to form constricted coated pits and, subsequently, for coatedvesicle budding.
Journal ArticleDOI
Caveolin-1 Null Mice Are Viable but Show Evidence of Hyperproliferative and Vascular Abnormalities
Babak Razani,Jeffery A. Engelman,Xiaobo Wang,William Schubert,Xiao Lan Zhang,Carolyn Marks,Frank P. Macaluso,Robert G. Russell,Maomi Li,Richard G. Pestell,Dolores Di Vizio,Harry Hou,Burkhard Kneitz,Guy Lagaud,George J. Christ,Winfried Edelmann,Michael P. Lisanti +16 more
TL;DR: The results indicate that eNOS activity is up-regulated in Cav-1 null animals, and this activity can be blunted by using a specific NOS inhibitor, nitro-l-arginine methyl ester.
Journal ArticleDOI
Regulated internalization of caveolae.
TL;DR: Although the exact mechanism of internalization remains unknown, the results show that caveolae are dynamic structures which can be internalized into the cell and may be regulated by kinase activity and require an intact actin network.