Showing papers on "Bioreactor published in 2019"
TL;DR: Results show that this bioreactor can achieve effective tumor colonization, and realize a self‐supplied therapeutic Fenton‐like reaction without additional H2O2 provision.
Abstract: Synthetic biology based on bacteria has been displayed in antitumor therapy and shown good performance. In this study, an engineered bacterium Escherichia coli MG1655 is designed with NDH-2 enzyme (respiratory chain enzyme II) overexpression (Ec-pE), which can colonize in tumor regions and increase localized H2 O2 generation. Following from this, magnetic Fe3 O4 nanoparticles are covalently linked to bacteria to act as a catalyst for a Fenton-like reaction, which converts H2 O2 to toxic hydroxyl radicals (•OH) for tumor therapy. In this constructed bioreactor, the Fenton-like reaction occurs with sustainably synthesized H2 O2 produced by engineered bacteria, and severe tumor apoptosis is induced via the produced toxic •OH. These results show that this bioreactor can achieve effective tumor colonization, and realize a self-supplied therapeutic Fenton-like reaction without additional H2 O2 provision.
249 citations
TL;DR: Sequential anaerobic and aerobic reactors are reviewed in order to present their advantages over single bioreactors and intermittent sequencing batch reactor is a promising technology than other high rate digesters in the removal of carbon, nitrogen, and phosphorous.
155 citations
TL;DR: The aim of this work was to study the performance of pollutants removal and biomass production by co-culture of Chlorella vulgaris and activated sludge in a batch photobioreactor (PBR), compared with their single system to treat a low C/N ratio wastewater.
81 citations
TL;DR: With the addition of QQB, the operating period of AnMBR-QQB reactor was prolonged by about 8-10 times at constant flux operation before reaching the pre-set maximum transmembrane pressure (TMP) and significant changes of organic functional groups were observed in cake layer from QQB membrane as compared with that from control membrane.
76 citations
TL;DR: This review highlights the advantages of using immobilized and co-immobilized cells together with continuous bioreactor configurations, which have the potential to improve both the yield and the green credentials of cellulosic ethanol production in modern industrial settings.
74 citations
TL;DR: The quantitative polymerase chain reaction (qPCR) analysis shows that tetracycline at μg/L level could greatly enhance the absolute and relative abundances of tetA, sulII, and blaTEM-1 in the effluent and aerobic granules, indicating tetrACYcline could serve as a selection pressure on the development of ARGs corresponding to different types of antibiotics in aerobic granule.
72 citations
TL;DR: The successful integration of AnMBRs in a treatment train that addresses the critical challenges of dissolved methane and nutrients demonstrates the viability of the technology in achieving holistic wastewater treatment.
Abstract: Concerns regarding ambient temperature operation, dissolved methane recovery, and nutrient removal have limited the implementation of anaerobic membrane bioreactors (AnMBRs) for domestic wastewater...
67 citations
TL;DR: In this article, the long-term performance of an upflow anaerobic sludge blanket reactor (UASB) and an AnMBR treating highly saline phenolic wastewater was evaluated.
65 citations
TL;DR: In this work, the agave bagasse was hydrothermally pretreated and optimized at 194 °C/30 min, obtaining a pretreated solid rich in cellulose content, and subjected to enzymatic hydrolysis at high solid levels.
65 citations
TL;DR: Results showed that aerobic granular sludge (AGS) was dominant in the bioreactor at day 45, and the relatively high protein content from tightly bound extracellular polymeric substances (TB-EPS) facilitated aerobic granulation and maintained biomass stabilization.
64 citations
TL;DR: Electro-assisted CNTs-HFMs served a dual function as the cathode and membrane filtration in the anaerobic electro-assisted membrane bioreactor (AnEMBR) for mitigating membrane fouling, improving water quality, and enhancing CH4 yield.
Abstract: A novel anaerobic treatment system that combines the impact of applied voltage with membrane filtration over carbon nanotubes hollow fiber membranes (CNTs-HFMs) was developed at low temperature (15-20 °C) to mitigate membrane fouling, treat wastewater, and recover energy (CH4). Herein, electro-assisted CNTs-HFMs served a dual function as the cathode and membrane filtration. In contrast with other two anaerobic membrane bioreactors (AnMBRs; polyvinylidene fluoride hollow fiber membranes and CNTs-HFMs without electro-assistance), the CNTs-HFMs with electro-assistance (-1.2 V applied voltage) had slower transmembrane pressure (TMP) increasing rates and better TMP recovery with a more than 95% effluent chemical oxygen demand (COD) removal rate during an almost 100-day operation period. This result can be attributed to the presence of an electrostatic repulsion force pushing pollutants (mainly extracellular polymeric substances, EPS) away from the membrane surface, thereby hindering the formation of a gel layer and mitigating membrane pore blocking in the anaerobic electro-assisted membrane bioreactor (AnEMBR). Due to the almost two-times higher Methanomicrobia content and more H2-utilizing methanogens than the other two AnMBRs, approximately more than 111.12 mL/gVSS d of CH4 was obtained in the AnEMBR with electro-assistance. This work provides an efficient strategy for mitigating membrane fouling, improving water quality, and enhancing CH4 yield.
TL;DR: A review of the most significant steps as well as current state-of-the-art of PTCC carried out in various types of bioreactor hardware used in PTCC.
Abstract: Bioreactors are engineered systems capable of supporting a biologically active situation for conducting aerobic or anaerobic biochemical processes. Stability, operational ease, improved nutrient uptake capacity, time- and cost-effectiveness, and large quantities of biomass production, make bioreactors suitable alternatives to conventional plant tissue and cell culture (PTCC) methods. Bioreactors are employed in a wide range of plant research, and have evolved over time. Such technological progress, has led to remarkable achievements in the field of PTCC. Since the classification of bioreactors has been extensively reviewed in numerous reviews, the current article avoids repeating the same material. Alternatively, it aims to highlight the principal advances in the bioreactor hardware s used in PTCC rather than classical categorization. Furthermore, our review summarizes the most significant steps as well as current state-of-the-art of PTCC carried out in various types of bioreactor.
TL;DR: Detailed analyses of reactor performance showed that the hydroquinone pathway might be the main route in the aerobic degradation of PNP, and two integrated membrane-aerated bioreactor systems were constructed to enhance PNP biodegradation.
TL;DR: In this paper, an industrial scale plant has been constructed for annual treatment of 657,000m3 real petrochemical wastewater of 4649,±′651′mg/l COD and more than 50,000 mg/l petrochemicals, where the plant system is divided into four parts, and the treatment process mainly functions in a 2200,m3 anaerobic expanded granular sludge bed (EGSB) bioreactor and a 10,000 m3 aerobic activated sludge (AS) Bioreactor with the hydraulic retention
TL;DR: The use of the lignocellulosic biomass (sugarcane bagasse) for producing a biofuel (ethanol) reduces the need for oil and is an environmental-friendly process.
TL;DR: This paper critically reviews some of the key studies on biomass enrichment via immobilisation of low growth yield microorganisms, high-rates via fully mixed conditions, technical developments in FBRs and ways of overcoming toxic effects via solution recycling.
TL;DR: The results indicate rapid adaptation to high-salt conditions (both NaCl and marine), and the capacity for PPB to form a combined wastewater treatment/resource recovery process, particularly for salty industrial wastewater.
TL;DR: Results showed that the system started-up in 27 days for brewery wastewater and then stably operated under various temperature, initial COD and pH conditions, which showed fast start-up and strong robustness, and provides a base for the scale-up of this novel technology.
TL;DR: An electrically assisted anaerobic system for chloramphenicol wastewater treatment was developed and electrical stimulation selected the dominant functional bacteria and increased antibiotic resistance in dominantfunctional bacteria, both of which enhanced CAP removal and methane production.
TL;DR: The process performance of the three-stage UASB system in terms of optimum COD loading rate and total energy yield was much higher than those of single and two-stage anaerobic processes.
TL;DR: Evaluated the performance of an inverse fluidized bed (IFB) bioreactor with anerobic biomass immobilized onto Kaldness-K1 media as the biosupport material for selenite removal and recovery as selenium nanoparticle from waste stream found ammonium bicarbonate was most suitable and lactate was found to be the preferred carbon substrate.
Abstract: This study evaluated the performance of an inverse fluidized bed (IFB) bioreactor with anerobic biomass immobilized onto Kaldness-K1 media as the biosupport material for selenite removal and recovery as selenium nanoparticle from waste stream. The effect of different process parameter and nutrient supplement on selenium removal was first studied in a batch system using serum bottles. The results revealed that among the different nitrogen substrates, ammonium bicarbonate was most suitable, whereas, lactate was found to be the preferred carbon substrate with 98% selenite removal efficiency and 90% COD removal. Later studies with the continuously operated IFB bioreactor operated at different hydraulic retention time (HRT) and different influent selenite concentrations revealed an efficient removal of selenite at 24 h HRT and 1.0 mM of influent Se (IV). Furthermore, selenium nanoparticles formed at the bottom of the bioreactor were successfully recovered with a recovery efficiency in the range 35–58% with a maximum recovery obtained at 1.0 mM selenite concentration and 24 h HRT. The extracellular produced selenium nanoparticles were found to be spherical in shape with 90–150 nm in size. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of extracellular polymeric substances (EPS) as capping agent on the outer surface of Se nanoparticles.
TL;DR: 13C isotope fractionations of CH4 and CO2 in biogas and microbial community composition were analyzed in 5 different feedstocks and it showed that grass silage, maize silage and swine manure fed reactors had similar δ 13C values and methanogenic community composition, dominated by Methanosarcinaceae.
TL;DR: This work explores for the first time the use of Deep Eutectic Solvents with phosphate buffer 100 mM pH 7 as cosolvent (10% v/v) in biocatalytic reactions in fed-batch and packed-bed bioreactors.
TL;DR: In this article, the authors compared the characteristics of EPS of nitritation- and nitrification-dominant processes in an integrated fixed-film activated sludge reactor, and showed that the tightly bound EPS (TB-EPS) were composed of polysaccharides and protein in variable ratios, while loosely bound EPS contained almost no protein.
TL;DR: A novel upflow anaerobic sludge-forward osmotic membrane bioreactor was developed for simultaneous wastewater treatment, membrane fouling reduction, and nutrient recovery.
TL;DR: Pilot results suggest a second clarifier for polishing, in addition to the Vs selector, may be needed in a full-scale application of the technique unless other downstream processes (flocculation, sedimentation, filtration) are provided to reach final water quality goals.
TL;DR: Two types of methane oxidation processes coupled to denitrification inside landfills: microaerobic and hypoxic methane oxidation coupled to Denitrification (MAME-D and HYME-D) are tested, which will expand the understanding of the environmental role of methanotrophs and methylOTrophs in both carbon and nitrogen cycles.
TL;DR: In this article, an up-flow anaerobic sludge blanket (UASB) bioreactor was used for the production of bio-hydrogen gas via mixed culture under thermophilic conditions.
TL;DR: The use of P. oxalicum, an ascomycetes fungus, for the biotransformation of DFC at flask and bench bioreactor scales reduces the acute toxicity of the medium supplemented with diclofenac and represents a novel and attractive alternative for the elimination of pharmaceutical compounds.
TL;DR: The optimal anMDBR temperature was found to be 45 °C, showing a balanced biogas production and membrane permeation performance including less fouling formation, in addition to its lower operation cost and smaller footprint compared with most other technologies for on-site wastewater treatment.