Elizabeth A. Worthey

TL;DR: The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Tritryp genomes suggest that the mechanisms regulating RNA polymerase II–directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling.

TL;DR: Although the Tritryp lack several classes of signaling molecules, their kinomes contain a large and diverse set of protein kinases and phosphatases; their size and diversity imply previously unknown interactions and regulatory processes, which may be targets for intervention.

TL;DR: No evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont is revealed, and a conserved core proteome of about 6200 genes in large syntenic polycistronic gene clusters is revealed.

TL;DR: The power of exome sequencing to render a molecular diagnosis in an individual patient in the setting of a novel disease, after all standard diagnoses were exhausted, is demonstrated and illustrates how this technology can be used in a clinical setting.

TL;DR: A genome-wide analysis of protein kinases of these three trypanosomatids reveals a large set of PKs, comprising approximately 2% of each genome, suggesting a key role for phosphorylation in parasite biology.