Refugio Robles-Sikisaka

TL;DR: A protocol for generating coding-sequence-complete genomes, comprising an online primer design tool, a novel multiplex PCR enrichment protocol, optimized library preparation methods for the portable MinION sequencer and the Illumina range of instruments, and a bioinformatics pipeline for generating consensus sequences.

TL;DR: It is found that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of surveillance of viral infections.

TL;DR: It is shown that at least 4 introductions, but potentially as many as 40, contributed to the outbreak in Florida and that local transmission is likely to have started in the spring of 2016—several months before its initial detection.

TL;DR: In this paper, the authors investigated the prevalence and dynamics of B.1.7 in the United States (US), tracking it back to its early emergence using S gene target failure (SGTF) and SARS-CoV-2 genomic sequencing.

TL;DR: Comparisons with mtCOI molecular clock calibrations suggest that nonsynonymous substitutions accumulate 2-50 times faster in lysin and 18-kDa genes than in rapidly evolving mammalian genes, which indicates that rapid adaptive evolution of lys in has occurred consistently through the history of the group.