W
William W. Metcalf
Researcher at University of Illinois at Urbana–Champaign
Publications - 167
Citations - 11242
William W. Metcalf is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Methanosarcina acetivorans & Methanosarcina. The author has an hindex of 57, co-authored 156 publications receiving 9737 citations. Previous affiliations of William W. Metcalf include Purdue University & Howard Hughes Medical Institute.
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Journal ArticleDOI
The Genome of M. Acetivorans Reveals Extensive Metabolic and Physiological Diversity
James E. Galagan,Chad Nusbaum,Alice Roy,Matthew G. Endrizzi,Matthew G. Endrizzi,Matthew G. Endrizzi,Pendexter Macdonald,Pendexter Macdonald,Pendexter Macdonald,William Fitzhugh,Sarah E. Calvo,Reinhard Engels,Serge Smirnov,Deven Atnoor,Adam Brown,Nicole R. Allen,Nicole R. Allen,Nicole R. Allen,Jerome Naylor,Nicole Stange-Thomann,Kurt DeArellano,Robin Johnson,Lauren Linton,Paul A. McEwan,Kevin McKernan,Jessica A. Talamas,Andrea Tirrell,Andrea Tirrell,Wenjuan Ye,Wenjuan Ye,Andrew Zimmer,Robert D. Barber,Isaac K. O. Cann,David E. Graham,David A. Grahame,Adam M. Guss,Reiner Hedderich,Cheryl Ingram-Smith,Cheryl Ingram-Smith,Cheryl Ingram-Smith,H. Craig Kuettner,Joseph A. Krzycki,Joseph A. Krzycki,John A. Leigh,Weixi Li,Jinfeng Liu,Biswarup Mukhopadhyay,John N. Reeve,Kerry S. Smith,Timothy A. Springer,Timothy A. Springer,Timothy A. Springer,Lowell Umayam,Owen White,Robert H. White,Everly Conway de Macario,Everly Conway de Macario,James G. Ferry,Ken F. Jarrell,Ken F. Jarrell,Hua Jing,Hua Jing,Hua Jing,Alberto J.L. Macario,Ian T. Paulsen,Ian T. Paulsen,Ian T. Paulsen,Matthew A. Pritchett,Kevin R. Sowers,Ronald V. Swanson,Steven H. Zinder,Steven H. Zinder,Eric S. Lander,Eric S. Lander,Eric S. Lander,William W. Metcalf,Bruce W. Birren +76 more
TL;DR: The complete genome sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A, is reported, which indicates the likelihood of undiscovered natural energy sources for methanogenesis, whereas the presence of single-subunit carbon monoxide dehydrogenases raises the possibility of nonmethanogenic growth.
Journal ArticleDOI
Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria.
William W. Metcalf,Weihong Jiang,Larry L. Daniels,Soo Ki Kim,Andreas Haldimann,Barry L. Wanner +5 more
TL;DR: Several new cloning vectors for mutagenesis and allele replacement experiments are described, including plasmids that have the R6K gamma DNA replication origin so they replicate only in bacteria supplying the pi replication protein, and which also encode a positive counterselectable marker.
Journal ArticleDOI
A computational framework to explore large-scale biosynthetic diversity
Jorge C. Navarro-Muñoz,Nelly Selem-Mojica,Michael W. Mullowney,Satria A. Kautsar,James H. Tryon,Elizabeth I. Parkinson,Elizabeth I. Parkinson,Emmanuel L. C. de los Santos,Marley Yeong,Pablo Cruz-Morales,Sahar Abubucker,Arne Roeters,Wouter Lokhorst,Antonio Fernandez-Guerra,Antonio Fernandez-Guerra,Antonio Fernandez-Guerra,Luciana Teresa Dias Cappelini,Anthony W. Goering,Regan J. Thomson,William W. Metcalf,Neil L. Kelleher,Francisco Barona-Gómez,Marnix H. Medema +22 more
TL;DR: Two bioinformatic tools enable sequence similarity network and phylogenetic analysis of gene clusters and their families across hundreds of strains and in large datasets, leading to the discovery of new natural products.
Journal ArticleDOI
A roadmap for natural product discovery based on large-scale genomics and metabolomics
James R. Doroghazi,Jessica C. Albright,Anthony W. Goering,Kou San Ju,Robert R. Haines,Konstantin A. Tchalukov,David P. Labeda,Neil L. Kelleher,William W. Metcalf +8 more
TL;DR: Extrapolation from the 830-genome dataset reveals that Actinobacteria encode hundreds of thousands of future drug leads, while the strong correlation between phylogeny and GCFs frames a roadmap to efficiently access them.
Journal ArticleDOI
Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria.
TL;DR: A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2 and preliminary results from other laboratories have confirmed the effectiveness of this method in additional phylogenetically diverse species.