Showing papers by "Government of India published in 2016"

State of newborn health in India

Abstract: About 0.75 million neonates die every year in India, the highest for any country in the world. The neonatal mortality rate (NMR) declined from 52 per 1000 live births in 1990 to 28 per 1000 live births in 2013, but the rate of decline has been slow and lags behind that of infant and under-five child mortality rates. The slower decline has led to increasing contribution of neonatal mortality to infant and under-five mortality. Among neonatal deaths, the rate of decline in early neonatal mortality rate (ENMR) is much lower than that of late NMR. The high level and slow decline in early NMR are also reflected in a high and stagnant perinatal mortality rate. The rate of decline in NMR, and to an extent ENMR, has accelerated with the introduction of National Rural Health Mission in mid-2005. Almost all states have witnessed this phenomenon, but there is still a huge disparity in NMR between and even within the states. The disparity is further compounded by rural–urban, poor–rich and gender differentials. There is an interplay of different demographic, educational, socioeconomic, biological and care-seeking factors, which are responsible for the differentials and the high burden of neonatal mortality. Addressing inequity in India is an important cross-cutting action that will reduce newborn mortality.

The Global strategy for women's, children's and adolescents' health (2016-2030): a roadmap based on evidence and country experience.

Abstract: Box 1 The Global strategy for women's, children's and adolescents' health (2016-2030) Objectives of the global strategy: * Survive: end preventable mortality; * Thrive: promote health and well-being; and * Transform: expand enabling environments. Five drivers of change to achieve the objectives based on the global strategy action areas: * People: individual potential and community engagement; * Political effectiveness: country leadership, financing, accountability; * Programmes: health system, multisector, humanitarian, research and innovation; * Partnerships: Every Woman Every Child Partnerships, Including the Global Financing Facility, the United Nations and multilateral H6 partnership, Unified Accountability Framework and Independent Accountability Panel, Innovation Marketplace and other national, regional and global partnerships; and * Principles: country-led, universal, sustainable, human-rights based, equity-driven, gender-responsive, evidence-informed, partnership-driven, people-centred, community-owned, accountable, aligned with development effectiveness and humanitarian norms. The Global strategy for women's, children's and adolescents' health (2016-2030) provides a roadmap for ending preventable deaths of women, children and adolescents by 2030 and helping them achieve their potential for and rights to health and well-being in all settings. (1) The global strategy has three objectives: survive (end preventable deaths); thrive (ensure health and well-being); and transform (expand enabling environments). These objectives are aligned with 17 targets within nine of the sustainable development goals (SDGs),2 including SDG 3 on health and other SDGs related to the political, social, economic and environmental determinants of health and sustainable development. Like the SDGs, the global strategy is universal in scope and multisectoral in action, aiming for transformative change across numerous challenging areas for health and sustainable development (Box 1). (1) The strategy was developed through evidence reviews and syntheses and a global stakeholder consultation, (3,4) and draws on new thinking about priorities and approaches for health and sustainable development. (4) Particular attention was given to experience gained and lessons learnt by countries during implementation of the previous Global strategy for women's and children's health (2010-2015) (5) and achieving the millennium development goals (MDGs). (6,7) A five-year operational framework with up-to-date technical resources has also been developed to support country-led implementation of the global strategy. This framework will be regularly updated until 2030. (1,3) Evidence shows that progress is required across a set of overlapping and mutually reinforcing areas to improve the health, dignity and well-being of women, children and adolescents. (4,7,8) Key areas for action were set out in the first global strategy (2010-2015), including health financing; the health system and workforce; access to essential interventions and life-saving commodities; national leadership; and accountability. (5) Based on emergent evidence, sociopolitical and environmental changes and the SDGs, the current global strategy (2016-2030) includes new strategic areas, for example adolescent health; humanitarian and fragile settings; an integrated life-course approach to health recognizing the links across different stages; multisector approaches; and guiding principles such as universality, human rights, equity and development effectiveness. (1) Evidence indicates that countries can accelerate progress in health and sustainable development through integrated action within the health sector and across social, economic and environmental sectors. (7,9) For example, through investments across sectors, the Chinese government lifted 439 million people out of poverty between 1990 and 2015, reduced child and maternal mortality by over 80% and 72%, respectively, and raised secondary school enrolment to over 99%, with equal numbers of boys and girls enrolled. …

Key issues in estimating energy and greenhouse gas savings of biofuels: challenges and perspectives

Abstract: The increasing demand for biofuels has encouraged the researchers and policy makers worldwide to find sustainable biofuel production systems in accordance with the regional conditions and needs. The sustainability of a biofuel production system includes energy and greenhouse gas (GHG) saving along with environmental and social acceptability. Life cycle assessment (LCA) is an internationally recognized tool for determining the sustainability of biofuels. LCA includes goal and scope, life cycle inventory, life cycle impact assessment, and interpretation as major steps. LCA results vary significantly, if there are any variations in performing these steps. For instance, biofuel producing feedstocks have different environmental values that lead to different GHG emission savings and energy balances. Similarly, land-use and land-use changes may overestimate biofuel sustainability. This study aims to examine various biofuel production systems for their GHG savings and energy balances, relative to conventional fossil fuels with an ambition to address the challenges and to offer future directions for LCA based biofuel studies. Environmental and social acceptability of biofuel production is the key factor in developing biofuel support policies. Higher GHG emission saving and energy balance of biofuel can be achieved, if biomass yield is high, and ecologically sustainable biomass or non-food biomass is converted into biofuel and used efficiently.

The number of privately treated tuberculosis cases in India: an estimation from drug sales data

Abstract: Summary Background Understanding the amount of tuberculosis managed by the private sector in India is crucial to understanding the true burden of the disease in the country, and thus globally. In the absence of quality surveillance data on privately treated patients, commercial drug sales data offer an empirical foundation for disease burden estimation. Methods We used a large, nationally representative commercial dataset on sales of 189 anti-tuberculosis products available in India to calculate the amount of anti-tuberculosis treatment in the private sector in 2013–14. We corrected estimates using validation studies that audited prescriptions against tuberculosis diagnosis, and estimated uncertainty using Monte Carlo simulation. To address implications for numbers of patients with tuberculosis, we explored varying assumptions for average duration of tuberculosis treatment and accuracy of private diagnosis. Findings There were 17·793 million patient-months (95% credible interval 16·709 million to 19·841 million) of anti-tuberculosis treatment in the private sector in 2014, twice as many as the public sector. If 40–60% of private-sector tuberculosis diagnoses are correct, and if private-sector tuberculosis treatment lasts on average 2–6 months, this implies that 1·19–5·34 million tuberculosis cases were treated in the private sector in 2014 alone. The midpoint of these ranges yields an estimate of 2·2 million cases, two to three times higher than currently assumed. Interpretation India's private sector is treating an enormous number of patients for tuberculosis, appreciably higher than has been previously recognised. Accordingly, there is a re-doubled need to address this burden and to strengthen surveillance. Tuberculosis burden estimates in India and worldwide require revision. Funding Bill & Melinda Gates Foundation.

Preprints for the life sciences

Abstract: A preprint is a complete scientific manuscript (often one also being submitted to a peer-reviewed journal) that is uploaded by the authors to a public server without formal review. After a brief inspection to ensure that the work is scientific in nature, the posted scientific manuscript can be viewed without charge on the Web. Thus, preprint servers facilitate the direct and open delivery of new knowledge and concepts to the worldwide scientific community before traditional validation through peer review ( 1 , 2 ). Although the preprint server arXiv.org has been essential for physics, mathematics, and computer sciences for over two decades, preprints are currently used minimally in biology.

Fern-like rGO/BiVO4 Hybrid Nanostructures for High-Energy Symmetric Supercapacitor

Abstract: Herein, we demonstrate the synthesis of rGO/BiVO4 hybrid nanostructures by facile hydrothermal method. Morphological studies reveal that rGO sheets are embedded in the special dendritic fern-like structures of BiVO4. The rGO/BiVO4 hybrid architecture shows the way to a rational design of supercapacitor, since these structures enable easy access of electrolyte ions by reducing internal resistance. Considering the unique morphological features of rGO/BiVO4 hybrid nanostructures, their supercapacitive properties were investigated. The rGO/BiVO4 electrode exhibits a specific capacitance of 151 F/g at the current density of 0.15 mA/cm2. Furthermore, we have constructed rGO/BiVO4 symmetric cell which exhibits outstanding volumetric energy density of 1.6 mW h/cm3 (33.7 W h/kg) and ensures rapid energy delivery with power density of 391 mW/cm3 (8.0 kW/kg). The superior properties of symmetric supercapacitor can be attributed to the special dendritic fern-like BiVO4 morphology and intriguing physicochemical proper...

Unexpected formation of N'-phenyl-thiophosphorohydrazidic acid O,S-dimethyl ester from acephate: chemical, biotechnical and computational study.

Abstract: By the nucleophilic attack of phenylhydrazine on acephate in aqueous medium, a stable product N′-phenyl-thiophosphorohydrazidic acid O,S-dimethyl ester (1) was obtained and characterized by elemental, spectral and thermal analysis. Thermodynamic parameters, Ea, ΔH°, ΔS° and ΔG°, have found to be 62.15, −67.95, −0.068 and −20.05 kJ mol−1 according to the Coats–Redfern equation. Analysis of interaction of 1 with BSA protein was done by using the UV–Vis and FTIR spectroscopic methods. The observed binding constants was 1.12 (±0.09) × 104 M−1. The biological effect of 1 was checked on different plant growth-promoting (PGPR) strains such as Rhizobium leguminosarum, Pseudomonas fluorescens, Arthrobacter citreus, Bacillus brevis and Salmonella typhimurium and compared with parent molecule acephate where 1 has shown less toxicity against PGPRs as compared to acephate. The experimental results for geometric parameters and values of peak position in IR spectra have found to match excellently with the computational studies performed by GAMESS software package. Theoretically, twelve new analogs of 1 were prepared and their comparative reactivity (HOMO–LUMO energy) and biodecomposition (on the basis of polarizability) is discussed.

Asymmetric Geometry of Defected Ground Structure for Rectangular Microstrip: A New Approach to Reduce its Cross-Polarized Fields

Abstract: A shaped defected ground structure (DGS) that is asymmetric in a specific radiating plane of a microstrip element has been explored with a view to addressing the cross-polarization (XP) issues. The asymmetric configuration has been conceived from an insight of the inherent asymmetry of the modal fields underneath a probe-fed microstrip patch and has been experimentally demonstrated as the best possible design compared to its predecessors in terms of the reduction in XP fields, angular span of suppression around boresight, and the space occupied by the defects. Different orientations of the defects with reference to the patches of different aspect ratio values have been thoroughly examined. More than 28-dB isolation between co-pol and cross-pol has been achieved over 190° angular range, which is indeed 140° more if compared with a conventional ground plane. Its superior characteristics with respect to the earlier designs have also been documented.

Molecular Characterization and Global Expression Analysis of Lectin Receptor Kinases in Bread Wheat (Triticum aestivum).

Abstract: Lectin receptor kinases (LRKs) play a critical role in plants during development and stress conditions, but a comprehensive analysis at genome level is still not carried out in Triticum aestivum. Herein, we performed the genome wide identification, characterization and expression analysis of these genes in T. aestivum (TaLRK). In-total 263 TaLRK genes were identified, which were further classified into three groups based on the nature of lectin domain. We identified, two TaLRKs consisted of calcium-dependent lectin (C-LRK), while 84 legume-lectin (L-LRK) and 177 bulb-lectin (B-LRK) domains. The L-LRK and B-LRK genes were distributed throughout the genome of T. aestivum. Most of the TaLRKs were clustered as homologs, which were distributed either in proximity on same chromosome or on homoeologous chromosomes of A, B and D sub-genomes. A total of 9 and 58 duplication events were also predicted in L-LRK and B-LRK, respectively. Phylogenetic analysis indicated conserved evolutionary relationship of homologous and orthologous genes from multiple plant species. Gene ontology analysis indicated TaLRKs role in binding, signaling and receptor activities. Most of the TaLRKs consisted of a trans-membrane domain and predicted to be localized in the plasma-membrane. A diverse expression pattern of TaLRK genes was found in various developmental stages and stress conditions. Some TaLRKs were found to be highly affected during a particular stress, which indicated a specialized role of each LRK gene in a specific stress condition. These results described various characteristic feature and expression pattern of TaLRK genes, which will pave the way for functional characterization in wheat.

Massive land system changes impact water quality of the Jhelum River in Kashmir Himalaya.

Abstract: The pristine aquatic ecosystems in the Himalayas are facing an ever increasing threat from various anthropogenic pressures which necessitate better understanding of the spatial and temporal variability of pollutants, their sources, and possible remedies. This study demonstrates the multi-disciplinary approach utilizing the multivariate statistical techniques, data from remote sensing, lab, and field-based observations for assessing the impact of massive land system changes on water quality of the river Jhelum. Land system changes over a period of 38 years have been quantified using multi-spectral satellite data to delineate the extent of different anthropogenically driven land use types that are the main non-point sources of pollution. Fifteen water quality parameters, at 12 sampling sites distributed uniformly along the length of the Jhelum, have been assessed to identify the possible sources of pollution. Our analysis indicated that 18% of the forested area has degraded into sparse forest or scrublands from 1972 to 2010, and the areas under croplands have decreased by 24% as people shifted from irrigation-intensive agriculture to orchard farming while as settlements showed a 397% increase during the observation period. One-way ANOVA revealed that all the water quality parameters had significant spatio-temporal differences (p < 0.01). Cluster analysis (CA) helped us to classify all the sampling sites into three groups. Factor analysis revealed that 91.84% of the total variance was mainly explained by five factors. Drastic changes in water quality of the Jhelum since the past three decades are manifested by increases in nitrate-nitrogen, TDS, and electric conductivity. The especially high levels of nitrogen (858 ± 405 μgL(-1)) and phosphorus (273 ± 18 μgL(-1)) in the Jhelum could be attributed to the reckless application of fertilizers, pesticides, and unplanned urbanization in the area.

Genotypic, Phenotypic and Clinical Validation of GeneXpert in Extra-Pulmonary and Pulmonary Tuberculosis in India.

Abstract: Background Newer molecular diagnostics have brought paradigm shift in early diagnosis of tuberculosis [TB]. WHO recommended use of GeneXpert MTB/RIF [Xpert] for Extra-pulmonary [EP] TB; critics have since questioned its efficiency. Methods The present study was designed to assess the performance of GeneXpert in 761 extra-pulmonary and 384 pulmonary specimens from patients clinically suspected of TB and compare with Phenotypic, Genotypic and Composite reference standards [CRS]. Results Comparison of GeneXpert results to CRS, demonstrated sensitivity of 100% and 90.68%, specificity of 100% and 99.62% for pulmonary and extra-pulmonary samples. On comparison with culture, sensitivity for Rifampicin [Rif] resistance detection was 87.5% and 81.82% respectively, while specificity was 100% for both pulmonary and extra-pulmonary TB. On comparison to sequencing of rpoB gene [Rif resistance determining region, RRDR], sensitivity was respectively 93.33% and 90% while specificity was 100% in both pulmonary and extra-pulmonary TB. GeneXpert assay missed 533CCG mutation in one sputum and dual mutation [517 & 519] in one pus sample, detected by sequencing. Sequencing picked dual mutation [529, 530] in a sputum sample sensitive to Rif, demonstrating, not all RRDR mutations lead to resistance. Conclusions Current study reports observations in a patient care setting in a high burden region, from a large collection of pulmonary and extra-pulmonary samples and puts to rest questions regarding sensitivity, specificity, detection of infrequent mutations and mutations responsible for low-level Rif resistance by GeneXpert. Improvements in the assay could offer further improvement in sensitivity of detection in different patient samples; nevertheless it may be difficult to improve sensitivity of Rif resistance detection if only one gene is targeted. Assay specificity was high both for TB detection and Rif resistance detection. Despite a few misses, the assay offers major boost to early diagnosis of TB and MDR-TB, in difficult to diagnose pauci-bacillary TB.

Smart Cities: A Global Perspective:

Abstract: The impact of globalisation and industrialisation has been a subject for research around the globe due to the huge paradigm shift caused by them. Such phenomena are also a cause of concern as citie...

Development of EMS-induced mutation population for amylose and resistant starch variation in bread wheat (Triticum aestivum) and identification of candidate genes responsible for amylose variation

Abstract: Starch is a major part of cereal grain. It comprises two glucose polymer fractions, amylose (AM) and amylopectin (AP), that make up about 25 and 75 % of total starch, respectively. The ratio of the two affects processing quality and digestibility of starch-based food products. Digestibility determines nutritional quality, as high amylose starch is considered a resistant or healthy starch (RS type 2) and is highly preferred for preventive measures against obesity and related health conditions. The topic of nutrition security is currently receiving much attention and consumer demand for food products with improved nutritional qualities has increased. In bread wheat (Triticum aestivum L.), variation in amylose content is narrow, hence its limited improvement. Therefore, it is necessary to produce wheat lines or populations showing wide variation in amylose/resistant starch content. In this study, a set of EMS-induced M4 mutant lines showing dynamic variation in amylose/resistant starch content were produced. Furthermore, two diverse mutant lines for amylose content were used to study quantitative expression patterns of 20 starch metabolic pathway genes and to identify candidate genes for amylose biosynthesis. A population comprising 101 EMS-induced mutation lines (M4 generation) was produced in a bread wheat (Triticum aestivum) variety. Two methods of amylose measurement in grain starch showed variation in amylose content ranging from ~3 to 76 % in the population. The method of in vitro digestion showed variation in resistant starch content from 1 to 41 %. One-way ANOVA analysis showed significant variation (p < 0.05) in amylose and resistant starch content within the population. A multiple comparison test (Dunnett’s test) showed that significant variation in amylose and resistant starch content, with respect to the parent, was observed in about 89 and 38 % of the mutant lines, respectively. Expression pattern analysis of 20 starch metabolic pathway genes in two diverse mutant lines (low and high amylose mutants) showed higher expression of key genes of amylose biosynthesis (GBSSI and their isoforms) in the high amylose mutant line, in comparison to the parent. Higher expression of amylopectin biosynthesis (SBE) was observed in the low amylose mutant lines. An additional six candidate genes showed over-expression (BMY, SPA) and reduced-expression (SSIII, SBEI, SBEIII, ISA3) in the high amylose mutant line, indicating that other starch metabolic genes may also contribute to amylose biosynthesis. In this study a set of 101 EMS-induced mutant lines (M4 generation) showing variation in amylose and resistant starch content in seed were produced. This population serves as useful germplasm or pre-breeding material for genome-wide study and improvement of starch-based processing and nutrition quality in wheat. It is also useful for the study of the genetic and molecular basis of amylose/resistant starch variation in wheat. Furthermore, gene expression analysis of 20 starch metabolic genes in the two diverse mutant lines (low and high amylose mutants) indicates that in addition to key genes, several other genes (such as phosphorylases, isoamylases, and pullulanases) may also be involved in contributing to amylose/amylopectin biosynthesis.

Gaps in Hypertension Guidelines in Low- and Middle-Income Versus High-Income Countries: A Systematic Review

Abstract: Hypertension, a leading cause of other cardiovascular diseases, is also a leading cause of disability and death worldwide.1 Over 1 billion people are diagnosed with hypertension, such that 1 in 3 individuals has elevated blood pressure in numerous countries.2 About 90% of the burden of cardiovascular disease is borne by the low-and middle-income countries (LMIC) that have only ≈10% of the research capacity and healthcare resources to confront the scourge.3 Hypertension had been regarded as a disease of the affluent people of the world.4,5 However, it has emerged in the LMIC where it affected ≈1 in 5 adults in 2013.5 This rate has been projected to increase such that 3 in 4 adults will be living with hypertension by 2025 in LMIC.6,7 Awareness and levels of hypertension control in LMIC are still low when compared with that in HIC.8 For instance, hypertension control in United States is 52% compared with 5% to 10% in Africa.9 The major reason for this disparity could be the lack of awareness of access and adherence to implementable hypertension guidelines in LMIC.10 Furthermore, hypertension management is complicated by choice, availability, and affordability of appropriate medications. The cultural aspects of life-long use of medications for hypertension, variable needs of individual patients, and inconsistent designs and outcomes from clinical trials have also compounded the management.11 The different genetic architectures of individuals with hypertension12,13 may determine the choice and response to treatment. Some of these antihypertensive agents are costly and not evenly accessible and distributed in LMIC. Therefore, guidelines that work in HIC settings may not be acceptable, effective, implementable, and applicable to LMIC because of the lack of supporting resources. In addition to broad international guidelines tailored to the needs …

Historical environmental change in Africa drives divergence and admixture of Aedes aegypti mosquitoes: a precursor to successful worldwide colonization?

Abstract: Increasing globalisation has promoted the spread of exotic species, including disease vectors. Understanding the evolutionary processes involved in such colonisations is both of intrinsic biological interest and important to predict and mitigate future disease risks. The Aedes aegypti mosquito is a major vector of dengue, chikungunya and Zika, the worldwide spread of which has been facilitated by Ae. aegypti's adaption to human-modified environments. Understanding the evolutionary processes involved in this invasion requires characterisation of the genetic makeup of the source population(s). The application of approximate Bayesian computation (ABC) to sequence data from four nuclear and one mitochondrial markers, revealed that African populations of Ae. aegypti best fit a demographic model of lineage diversification, historical admixture and recent population structuring. Since ancestral Ae. aegypti were dependent on forests, this population history is consistent with the effects of forest fragmentation and expansion driven by Pleistocene climatic change. Alternatively, or additionally, historical human movement across the continent may have facilitated their recent spread and mixing. ABC analysis and haplotype networks support earlier inferences of a single out of Africa colonisation event, while a cline of decreasing genetic diversity indicates that Ae. aegypti moved first from Africa to the Americas and then to Asia. ABC analysis was unable to verify this colonisation route, possibly because the genetic signal of admixture obscures the true colonisation pathway. By increasing genetic diversity and forming novel allelic combinations, divergence and historical admixture within Africa could have provided the adaptive potential needed for the successful worldwide spread of Ae. aegypti. This article is protected by copyright. All rights reserved.

Adoption of Electronic Health Records: A Roadmap for India.

Abstract: Objectives The objective of the study was to create a roadmap for the adoption of Electronic Health Record (EHR) in India based an analysis of the strategies of other countries and national scenarios of ICT use in India. Methods The strategies for adoption of EHR in other countries were analyzed to find the crucial steps taken. Apart from reports collected from stakeholders in the country, the study relied on the experience of the author in handling several e-health projects. Results It was found that there are four major areas where the countries considered have made substantial efforts: ICT infrastructure, Policy & regulations, Standards & interoperability, and Research, development & education. A set of crucial activities were identified in each area. Based on the analysis, a roadmap is suggested. It includes the creation of a secure health network; health information exchange; and the use of open-source software, a national health policy, privacy laws, an agency for health IT standards, R&D, human resource development, etc. Conclusions Although some steps have been initiated, several new steps need to be taken up for the successful adoption of EHR. It requires a coordinated effort from all the stakeholders.

Confinement of Ag3PO4 nanoparticles supported by surface plasmon resonance of Ag in glass: Efficient nanoscale photocatalyst for solar H2 production from waste H2S

Abstract: Ag 3 PO 4 is a good photocatalyst but ubiquitously known for its photocorrosion problem during photocatalytic reaction. Therefore, stabilization of Ag 3 PO 4 with retaining its fundamental properties has immense importance. With this motivation, we designed Ag 3 PO 4 glass nanocomposite to resolve the problem of photocorrosion. Moreover, the effect of size quantization on photocatalytic activity has also been demonstrated by growing the cubic Ag 3 PO 4 nanoparticles with size in the range of 3–9 nm in glass matrix via melt and quenching method. The band gap of Ag 3 PO 4 has been tuned (2.56–2.25 eV) in glass matrix with respect to size. Considering the size tunable band gap of Ag 3 PO 4 glass nanocomposite within visible region, it is demonstrated as a photocatalyst for hydrogen (H 2 ) production from copious hazardous waste H 2 S. The utmost H 2 production i.e. 3920.4 μmol h −1 g −1 is obtained using 1 gm of Ag 3 PO 4 glass nanocomposite powder. The apparent quantum yield for H 2 production is calculated to be 5.51% for Ag 3 PO 4 glass nanocomposite. Interestingly, presence of plasmonic Ag was also observed in Ag 3 PO 4 glass nanocomposite which contributes for H 2 production through enhanced light absorption, efficient charge separation and improved stability. Recycling study of sample reveals stable H 2 production efficiency and good stability of the photocatalyst. Surprisingly, catalyst can be reused many times and recovery of catalyst is possible just rinsing with distilled water. All these results demonstrate directly the feasibility of designing a new generation photocatalysts.

Inpatient care of small and sick newborns in healthcare facilities.

Abstract: Neonatal units in teaching and non-teaching hospitals both in public and private hospitals have been increasing in number in the country since the sixties. In 1994, a District Newborn Care Programme was introduced as a part of the Child Survival and Safe Motherhood Programme (CSSM) in 26 districts. Inpatient care of small and sick newborns in the public health system got a boost under National Rural Health Mission with the launch of the national programme on facility-based newborn care (FBNC). This has led to a nationwide creation of Newborn Care Corners (NBCC) at every point of child birth, newborn stabilization units (NBSUs) at First Referral Units (FRUs) and special newborn care units (SNCUs) at district hospitals. Guidelines and toolkits for standardized infrastructure, human resources and services at each level have been developed and a system of reporting data on FBNC created. Till March 2015, there were 565 SNCUs, 1904 NBSUs and 14 163 NBCCs operating in the country. There has been considerable progress in operationalizing SNCUs at the district hospitals; however establishing a network of SNCUs, NBSUs and NBCCs as a composite functional unit of newborn care continuum at the district level has lagged behind. NBSUs, the first point of referral for the sick newborn, have not received the desired attention and have remained a weak link in most districts. Other challenges include shortage of physicians, and hospital beds and absence of mechanisms for timely repair of equipment. With admission protocols not being adequately followed and a weak NBSU system, SNCUs are faced with the problem of admission overload and poor quality of care. Applying best practices of care at SNCUs, creating more NBSU linkages and strengthening NBCCs are important steps toward improving quality of FBNC. This can be further improved with regular monitoring and mentoring from experienced pediatricians, and nurses drawn from medical colleges and the private sector. In addition there is a need to further increase such units to address the unmet need of facility-based care.

Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains.

Abstract: Approaches enabling efficient phosphorus utilization in crops are of great importance. In cereal crop like wheat, utilization of inorganic phosphate (Pi) is high and mature grains are the major sink for Pi utilization and storage. Research that addresses the importance of the Pi homeostasis in developing grains is limited. In an attempt to understand the Pi homeostasis in developing wheat grains, we identified twelve new phosphate transporters (PHT), these are phyologentically well distributed along with the members reported from Arabidopsis and rice. Enhanced expression of PHT1-subfamily genes was observed in roots subjected to the Pi starvation suggesting their active role in Pi homeostasis. Differential expression patterns of all the PHT genes during grain filling stages suggested their importance in the filial tissues. Additionally, high accumulation of Pi and total P in aleurone correlates well with the expression of TaPHTs and other phosphate starvation related genes. Tissue specific transcript accumulation of TaPHT1.1, TaPHT1.2, TaPHT1.4 in aleurone; TaPHT3.1 in embryo and TaPHT4.2 in the endosperm was observed. Furthermore, their transcript abundance was affected in low phytate wheat grains. Altogether, this study helps in expanding the knowledge and prioritize the candidate wheat Pi-transporters to modulate the Pi homeostasis in cereal grains.

The How Project: understanding contextual challenges to global surgical care provision in low-resource settings

Abstract: Introduction 5 billion people around the world do not have access to safe, affordable, timely surgical care. This series of qualitative interviews was launched by The Lancet Commission on Global Surgery (LCoGS) with the aim of understanding the contextual challenges—the specific circumstances—faced by surgical care providers in low-resource settings who care for impoverished patients, and how those providers overcome these challenges. Methods From January 2014 to February 2015, 20 LCoGS collaborators conducted semistructured interviews with 148 surgical providers in low-resource settings in 21 countries. Stratified purposive sampling was used to include both rural and urban providers, and reputational case selection identified individuals. Interviewers were trained with an implementation manual. Following immersion into de-identified texts from completed interviews, topical coding and further analysis of coded texts was completed by an independent analyst with periodic validation from a second analyst. Results Providers described substantial financial, geographic and cultural barriers to patient access. Rural surgical teams reported a lack of a trained workforce and insufficient infrastructure, equipment, supplies and banked blood. Urban providers face overcrowding, exacerbated by minimal clinical and administrative support, and limited interhospital care coordination. Many providers across contexts identified national health policies that do not reflect the realities of resource-poor settings. Some findings were region-specific, such as weak patient–provider relationships and unreliable supply chains. In all settings, surgical teams have created workarounds to deliver care despite the challenges. Discussion While some differences exist between countries, the barriers to safe surgery and anaesthesia are overall consistent and resource-dependent. Efforts to advance and expand global surgery must address these commonalities, while local policymakers can tailor responses to key contextual differences.

Role of debris cover to control specific ablation of adjoining Batal and Sutri Dhaka glaciers in Chandra Basin (Himachal Pradesh) during peak ablation season

Abstract: As part of the on-going annual mass balance measurements on Batal and Sutri Dhaka glaciers, observations were made during peak ablation (August–September) season in 2013 to understand the response of debris covered and clean-ice (debris free) glacier surface to melting processes. Though, both the Batal and Sutri Dhaka glaciers have almost similar geographical disposition, Batal shows extensive debris cover (90% of the ablation area), while the latter is free from debris (only 5% of the ablation area). The thickness of debris in Batal glacier is inversely proportional to altitude, whereas Sutri Dhaka mostly experienced debris-free zone except snout area. Observation revealed that the vertical gradient of ablation rate in ablation area is contrastingly opposite in these two glaciers, reflecting significant control of debris thickness and their distribution over glacier surface on the ablation rates. While different thickness (2–100 cm) of debris have attenuated melting rates up to 70% of total melting, debris cover of <2 cm thickness has accelerated melting up to 10% of the total melting. Estimated melt ratio reveals that about 90% of the ablation area has experienced inhibited melting in Batal glacier, whereas only less than 5% ablation area of Sutri Dhaka has undergone inhibited melting. Comparison of topographical maps of 1962 with successive satellite images of the area demonstrates a terminus retreat of 373 ± 33.5 m and 579 ± 33.5 m for Batal and Sutri Dhaka glaciers for the period 1962–2013, respectively.

Genome-wide Expression Analysis and Metabolite Profiling Elucidate Transcriptional Regulation of Flavonoid Biosynthesis and Modulation under Abiotic Stresses in Banana.

Abstract: Flavonoid biosynthesis is largely regulated at the transcriptional level due to the modulated expression of genes related to the phenylpropanoid pathway in plants. Although accumulation of different flavonoids has been reported in banana, a staple fruit crop, no detailed information is available on regulation of the biosynthesis in this important plant. We carried out genome-wide analysis of banana (Musa acuminata, AAA genome) and identified 28 genes belonging to 9 gene families associated with flavonoid biosynthesis. Expression analysis suggested spatial and temporal regulation of the identified genes in different tissues of banana. Analysis revealed enhanced expression of genes related to flavonol and proanthocyanidin (PA) biosynthesis in peel and pulp at the early developmental stages of fruit. Genes involved in anthocyanin biosynthesis were highly expressed during banana fruit ripening. In general, higher accumulation of metabolites was observed in the peel as compared to pulp tissue. A correlation between expression of genes and metabolite content was observed at the early stage of fruit development. Furthermore, this study also suggests regulation of flavonoid biosynthesis, at transcriptional level, under light and dark exposures as well as methyl jasmonate (MJ) treatment in banana.

Studies on nanoparticle induced nutrient use eficiency of fertilizer and crop productivity

Abstract: A field experiment was conducted at M/s.Rashtriya Chemicals and Fertilizers, Ltd., Mumbai, India, (RCF) experimental farm to evaluate the effect of ZnO Nanoparticles (ZnO NP) in combination with N: P: K (15: 15:15) complex fertilizer “Suphala” of RCF Ltd. on growth attributes of brinjal (Solanum melongena L) as well as nutrient use efficiency. The experiment was carried out in randomised block design with three replications. The first treatment (T-1), comprised of recommended dose of fertilizer (RDF), N: P: K (50:50:50), applied at the time of transplantation. The second treatment (T-2) was conducted with RDF in combination @ 2kg ZnSO4 (bulk)/ha. The third treatment (T-3) was added, N: P: K (12.5; 12.5; 12.5) in combination to ZnO NP @ 4500mg/ha. The forth treatment (T-C) was without any fertilizer. All treatments were given appropriate quantity of nitrogen per hectare as urea at the 30th day of transplantation. The combination N: P: K (12.5; 12.5; 12.5) and ZnO NP @ 4500mg/ha yielded 91% and 45.3% higher brinjal yield and biomass respectively than the treatment with only RDF. It was also observed that 38% and 21% higher yield and biomass respectively were recorded in the treatment where combination of RDF with ZnSO4 (bulk) over RDF was used alone. The results of field trials reveal that, there was synergistic effect of ZnO NP @ 4500mg per hectare with N: P: K complex fertilizer on growth attributes of brinjal as well as nutrient use efficiency.

Experience of active tuberculosis case finding in nearly 5 million households in India

Abstract: In India, to increase tuberculosis (TB) case detection under the National Tuberculosis Programme, active case finding (ACF) was implemented by the Global Fund-supported Project Axshya, among high-risk groups in 300 districts. Between April 2013 and December 2014, 4.9 million households covering ~20 million people were visited. Of 350 047 presumptive pulmonary TB cases (cough of ⩾2 weeks) identified, 187 586 (54%) underwent sputum smear examination and 14 447 (8%) were found to be smear-positive. ACF resulted in the detection of a large number of persons with presumptive pulmonary TB and smear-positive TB. Ensuring sputum examination of all those with presumptive TB was a major challenge.