Hasanuddin University

About: Hasanuddin University is a education organization based out in Makassar, Indonesia. It is known for research contribution in the topics: Population & Medicine. The organization has 5839 authors who have published 5267 publications receiving 25893 citations. The organization is also known as: Universitas Hasanuddin.

Niveles de interleucina-6 en el humor acuoso en glaucoma primario de ángulo abierto (GPAA): revisión sistemática y meta-análisis

Abstract: Resumen Objetivo determinar el cambio en los niveles de interleucina-6 (IL-6) en el humor acuoso entre pacientes con glaucoma primario de angulo abierto (GPAA). Metodos se recuperaron estudios que investigaron los niveles de IL-6 en el humor acuoso de pacientes con GPAA utilizando los metodos de deteccion de ensayo inmunosorbente ligado a enzimas (ELISA) e inmunoensayo de microesferas. Se realizo un meta-analisis para determinar el cambio global de IL-6 en pacientes de GPAA comparados con controles. Resultados se seleccionaron 14 estudios en total. El analisis combinando los resultados de estudios con ambos metodos de deteccion no mostro cambios en el nivel de IL-6 en GPAA (SMD = -0,07, 95%IC -0,73 – 0,59, P = 0,83), posiblemente debido a la heterogeneidad de los datos de ELISA. Puesto que el inmunoensayo de microesferas es mas sensible que el ELISA convencional para detectar anticuerpos, los resultados de inmunoensayos de microesferas aislados mostraron que los pacientes GPAA tenian un nivel inferior de IL-6 en el humor acuoso comparado con controles (SMD = -0,40, 95%IC -0,70 – 0,09, P = 0,01). Asimismo, una prueba de sensibilidad tambien confirmo que no se observaron alteraciones de los resultados en todos los estudios agrupados y tambien en los estudios de inmunoensayo de microesferas agrupados, sugiriendo la estabilidad de los resultados sinteticos del presente estudio. Conclusion debido a su estabilidad, los datos agrupados del inmunoensayo de microesferas se utilizaron para obtener la conclusion de este estudio, demostrando que la reduccion de los niveles de IL-6 en el humor acuoso se pudo observar en pacientes con GPAA. Es necesario llevar a cabo estudios adicionales para confirmar estos hallazgos.

The complete genomes of subgenotype IA hepatitis A virus strains from four different islands in Indonesia form a phylogenetic cluster

Abstract: Despite the high endemicity of hepatitis A virus (HAV) in Indonesia, genetic information on those HAV strains is limited. Serum samples obtained from 76 individuals during outbreaks of hepatitis A in Jember (East Java) in 2006 and Tangerang (West Java) in 2007 and those from 82 patients with acute hepatitis in Solo (Central Java), Denpasar on Bali Island, Mataram on Lombok Island, and Makassar on Sulawesi Island in 2003 or 2007 were tested for the presence of HAV RNA by reverse transcription PCR with primers targeting the VP1-2B region (481 nucleotides, primer sequences at both ends excluded). Overall, 34 serum samples had detectable HAV RNA, including at least one viremic sample from each of the six regions. These 34 strains were 96.3-100 % identical to each other and formed a phylogenetic cluster within genotype IA. Six representative HAV isolates from each region shared 98.3-98.9 % identity over the entire genome and constituted a IA sublineage with a bootstrap value of 100 %, consisting of only Indonesian strains. HAV strains recovered from Japanese patients who were presumed to have contracted HAV infection while visiting Indonesia were closest to the Indonesian IA HAV strains obtained in the present study, with a high identity of 99.5-99.7 %, supporting the Indonesian origin of the imported strains. These results indicate that genetic analysis of HAV strains indigenous to HAV-endemic countries, including Indonesia, are useful for tracing infectious sources in imported cases of acute hepatitis A and for defining the epidemiological features of HAV infection in that country.

Role and function of gelatin in the development of the food and non-food industry: A review

Abstract: Gelatin is a product of the partial hydrolysis of collagen in livestock. Gelatin has been used extensively in the food and non-food industries. Gelatin has a hydrocolloid form and plays an important role in influencing the properties of industrial products. Global gelatin demands is increasing every year along with the increasing human need for food and non-food industrial products. The properties of gelatin are influenced by the properties of collagen. Collagen is a derivative of fibrous protein which has an enormous role and benefit in forming gelatin molecules. Collagen is a long polypeptide chain that makes up about 50 to 1000 amino acid chains. The amino acid chain is dominated by the amino acids glycine, proline and hydroxyproline. Various studies related to the properties of gelatin produced from livestock and fish tissues have developed rapidly. Currently researchers have developed livestock and fish waste as an alternative source of gelatin. Alternative sources of gelatin have been widely published as an effort to find halal and hygienic sources of gelatin.

Brucellosis seroprevalence in Bali cattle with reproductive failure in South Sulawesi and Brucella abortus biovar 1 genotypes in the Eastern Indonesian archipelago.

Abstract: Brucellosis is a major cause of infertility and reproductive failure in livestock. While cattle in the Eastern Indonesian archipelago suffers from reproductive problems information on bovine brucellosis in the region is fragmentary. The control of brucellosis requires a major and prolonged effort and confirmation of the infection by isolation with detailed knowledge of the spread of the infection is essential when planning a control program. Serological investigation of Brucella infection in beef cattle tended under extensive farming conditions revealed a high seroprevalence (19.3%; 95% CI, 17–22) in the compliment fixation tests. The results of a rapid and simple field test correlated well with the Rose Bengal test (kappa, 0.917) and indicated an acceptable sensitivity (87.5%) and specificity (98.1%) compared with the complement fixation test. Reproductive failure was reported for 39.0% of the cows with a loss of calves due to abortion or early death amounting to 19.3%. Past reproductive failure did not, however, correlate with seropositivity in the complement fixation test (RP = 1.21; P = 0.847). B. abortus biovar 1 was freshly isolated from the hygromas of two cows and together with thirty banked isolates collected since 1990 from different parts of Sulawesi and Timor eight related genotypes could be distinguished with one genotype being identical to that of an isolate (BfR91) from Switzerland. The Indonesian genotypes formed together with BfR91 and one African and one North American isolate a distinct branch on the B. abortus biovar 1 dendogram. Bovine brucellosis appears to be widespread in the Eastern Indonesian archipelago and calls for urgent intervention. The fresh isolation of the pathogen together with the observed high seroprevalence demonstrates the presence and frequent exposure of cattle in the area to the pathogen. The application of a rapid and simple field test for brucellosis could be very useful for the quick screening of cattle at the pen side.