Institution
Institut national de la recherche agronomique
Facility•Rabat, Morocco•
About: Institut national de la recherche agronomique is a facility organization based out in Rabat, Morocco. It is known for research contribution in the topics: Population & Gene. The organization has 41515 authors who have published 68362 publications receiving 3292057 citations. The organization is also known as: INRA & Inra.
Topics: Population, Gene, Soil water, Genome, Quantitative trait locus
Papers published on a yearly basis
Papers
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TL;DR: The concept of normalization in transcript quantification is introduced here in an attempt to convince molecular biologists, and non-specialists, that systematic validation of reference genes is essential for producing accurate, reliable data in qRT-PCR analyses, and thus should be an integral component of them.
Abstract: Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical algorithms have been developed recently to help validate reference genes but, surprisingly, this robust approach is under-utilized in plants. Instead, putative 'housekeeping' genes tend to be used as references without any proper validation. The concept of normalization in transcript quantification is introduced here and the factors affecting its reliability in qRT-PCR are discussed in an attempt to convince molecular biologists, and non-specialists, that systematic validation of reference genes is essential for producing accurate, reliable data in qRT-PCR analyses, and thus should be an integral component of them.
524 citations
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TL;DR: The aim of the present investigation was to improve the extraction, purification, and quantification of DNA derived from as large a portion of the soil microbial community as possible, with special emphasis placed on obtaining DNA from gram-positive bacteria, which form structures that are difficult to disrupt.
Abstract: In recent years, several protocols based on the extraction of nucleic acids directly from the soil matrix after lysis treatment have been developed for the detection of microorganisms in soil. Extraction efficiency has often been evaluated based on the recovery of a specific gene sequence from an organism inoculated into the soil. The aim of the present investigation was to improve the extraction, purification, and quantification of DNA derived from as large a portion of the soil microbial community as possible, with special emphasis placed on obtaining DNA from gram-positive bacteria, which form structures that are difficult to disrupt. Furthermore, we wanted to identify and minimize the biases related to each step in the procedure. Six soils, covering a range of pHs, clay contents, and organic matter contents, were studied. Lysis was carried out by soil grinding, sonication, thermal shocks, and chemical treatments. DNA was extracted from the indigenous microflora as well as from inoculated bacterial cells, spores, and hyphae, and the quality and quantity of the DNA were determined by gel electrophoresis and dot blot hybridization. Lysis efficiency was also estimated by microscopy and viable cell counts. Grinding increased the extracellular DNA yield compared with the yield obtained without any lysis treatment, but none of the subsequent treatments clearly increased the DNA yield. Phage λ DNA was inoculated into the soils to mimic the fate of extracellular DNA. No more than 6% of this DNA could be recovered from the different soils. The clay content strongly influenced the recovery of DNA. The adsorption of DNA to clay particles decreased when the soil was pretreated with RNA in order to saturate the adsorption sites. We also investigated different purification techniques and optimized the PCR methods in order to develop a protocol based on hybridization of the PCR products and quantification by phosphorimaging.
523 citations
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TL;DR: A consumer-driven definition for the concept of TFP and innovation was obtained and these across six European countries (Belgium, France, Italy, Norway, Poland and Spain) by means of semantic and textual statistical analyses.
523 citations
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TL;DR: The transcriptional profile of the mucosa appears to interact with the colonic microbiota; this interaction appears to be lost in colon of patients with UC, which has different gene expression profiles and lower levels of biodiversity than their healthy twins.
522 citations
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TL;DR: The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination and high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption.
Abstract: We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.
522 citations
Authors
Showing all 41526 results
Name | H-index | Papers | Citations |
---|---|---|---|
Daniel J. Jacob | 162 | 656 | 76530 |
Jens J. Holst | 160 | 1536 | 107858 |
Grant W. Montgomery | 157 | 926 | 108118 |
Dirk Inzé | 149 | 647 | 74468 |
Bernard Henrissat | 139 | 593 | 100002 |
David Julian McClements | 131 | 1137 | 71123 |
Pascale Cossart | 124 | 434 | 50101 |
Christine H. Foyer | 116 | 490 | 61381 |
Eric Verdin | 115 | 370 | 47971 |
Olivier Hermine | 111 | 1026 | 43779 |
John Ralph | 109 | 442 | 39238 |
Edward M. Rubin | 107 | 287 | 62667 |
Gary Williamson | 106 | 478 | 42960 |
Stephen L. Hauser | 106 | 561 | 46248 |
Serge Hercberg | 106 | 942 | 56791 |