Showing papers by "University of Catania published in 1995"

p53-independent induction of WAF1/CIP1 in human leukemia cells is correlated with growth arrest accompanying monocyte/macrophage differentiation

Abstract: The p53 tumor suppressor gene plays a role in controlling a G1 phase checkpoint. The WAF1/CIP1 gene with encodes p21WAF1/CIP1 protein, an inhibitor of cyclin-dependent kinases, is a downstream mediator of p53 function. We examined expression of the WAF1/CIP1 gene and its relationship to growth arrest and differentiation in p53-null human leukemic cell lines. We show that p53-independent induction of WAF1/CIP1 occurs in human leukemia cells treated with 12-O-tetradecanoylphorbol-13-acetate, okadaic acid, or IFN-γ but not with retinoic acid, vitamin D3, or DMSO. Furthermore, WAF1/CIP1 induction correlates with growth arrest associated with monocyte-macrophage differentiation. The present studies support the idea that WAF1/CIP1 gene expression can be regulated through multiple mechanisms, suggesting that strategies may be designed to restore the G1 checkpoint controls in p53-null cells by targeting these p53-independent mechanisms of WAF1/CIP1 induction.

Photodesorption from low-temperature water ice in interstellar and circumsolar grains

Abstract: DUST grains in the interstellar medium1 and the outer Solar System2–4 commonly have a coating of water ice, which affects their optical properties and surface chemistry. The thickness of these icy mantles may be determined in part by the extent of photodesorption (photosputtering) by background ultraviolet radiation. But this process is poorly understood, with theoretical estimates of the photodesorption rate spanning several orders of magnitude5,6. Here we report measurements of the absolute ultraviolet photodesorption yield of low-temperature water ice. Our results indicate that the rate of photodesorption is appreciable. In particular, it can account for the absence of icy mantles on grains in diffuse interstellar clouds, it exceeds solar-wind ion erosion and sublimation in the outer Solar System, and it is important in determining the lifetimes of icy mantles in dense molecular clouds.

Characterization of polymers by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry: Molecular weight estimates in samples of varying polydispersity

Abstract: Matrix-assisted laser desorptionhonization time-of-flight mass spectrometry (MALDI-TOF MS) allows the detection of large molecules such as those present in synthetic and natural macromolecules. Synthetic polymers may show a wide range of molecular weight distributions (MWD), according to the synthetic method used in their preparation. We have studied several poly(methylmethacry1ate) (PMMA), poly(styrene) (PS) and poly(ethyleneg1ycol) (PEG) samples with varying MWD, and also a number of condensation polymers such as Nylon-6, poly(carbonate) and poly(ester). Measurements of MALDI-TOF spectra in the linear mode were used in our work to estimate the molecular weight (MW) and MWD of the polymer samples. Our results show that the molecular weight estimates provided by MALDI-TOF measurements agree with the values obtained by conventional techniques, such as gel-permeation chromatography (GPC), only in the case of polymer samples with very narrow molecular weight distribution. However, when the polydispersity reaches values around 1.10 the difference between the MW measured by GPC and measured by MALDI may amount to up to about 20%. At higher dispersions, the MALDI spectra fail to yield reliable MW values.

Association of a chromosome deletion syndrome with a fragile site within the proto-oncogene CBL2

Abstract: The fragile site FRA11B has been localized to the p(CCG)n repeat of the CBL2 proto-oncogene. A proportion of Jacobsen (11q-) syndrome patients inherited a chromosome carrying a CBL2 p(CCG)n expansion, which was truncated close to FRA11B. These results have broad implications for the role of p(CCG)n repeat expansion in the aetiology of genetic disease involving chromosome rearrangements.

Tectonics and sequence stratigraphy in Messinian basins, Sicily: Constraints on the initiation and termination of the Mediterranean salinity crisis

Abstract: Deposits within the Caltanissetta basin of central Sicily have been important for developing the desiccating deep basin model for the Messinian salinity crisis in the Mediterranean. Linked structural and stratigraphic studies show that the Sicilian depocenters are synclines related to underlying thrust structures of the frontal part of the Maghrebian chain. Prior to the salinity crisis these basins were hydrodynamically linked through the foredeep to the Mediterranean. The precursor sediments (Terravecchia Formation) formed a delta, sourced from the north. Early Messinian regression acted on a range of paleobathymetries, shallow in the north and progressively deeper in the south, locally complicated by active thrust structures. Initial draw-down of Mediterranean base level is marked across the thrust belt by first cycle carbonates and evaporites on the structural highs and lows, respectively. Vast accumulations of halite and potassium salts (up to 1200 m) are restricted to growing thrust synclines. The different evaporite signatures may be related to different water conditions reflecting various meteoric and marine circulations across various subbasins, with cyclicity reflecting high-frequency variations in sea level. A sequence stratigraphic model is developed to explain fractionation of evaporite facies between different paleobathymetric settings and to predict the temporal evolution of the successions in different subbasins. Thrusting provides accommodation space for evaporites and also controls the water pathways into the desiccating basins. The intra-Messinian unconformity separating first and second cycle evaporites is a “type 1 sequence boundary” related to the forced regression associated with the acme of Mediterranean desiccation, an interpretation supported by local ravinement and incised valley fills. The overlying second cycle evaporites are a combination of detrital, reworked first cycle material and primary gypsum formed under brackish water. Regional onlap relationships and bed continuity suggest that this water body was of regional extent with a systematically rising base level. These interpretations have several implications for Messinian correlations in the Mediterranean: The deep basin evaporites correlate with an intra-Messinian unconformity that separates the two cycles on Sicily and postdate the first cycle deposits; regional base level was largely restored before the end of the salinity crisis.

Selective Lesioning of the Basal Forebrain Cholinergic System by Intraventricular 192 IgG–saporin: Behavioural, Biochemical and Stereological Studies in the Rat

Abstract: The elucidation of the functional role of the basal forebrain cholinergic system will require access to a highly specific and efficient cholinergic neurotoxin. Recently, selective depletion of the nerve growth factor (NGF) receptor-bearing cholinergic neurons in the rat basal forebrain and a dramatic loss of cholinergic innervation in the related cortical regions have been obtained following intraventricular injection of a newly introduced immunotoxin, 192 IgG-saporin. Here we extend these initial findings and report that administration of increasing doses (1.25, 2.5, 5.0 or 10 micrograms) of the 192 IgG-saporin conjugate into the lateral ventricles of adult rats induced dose-dependent impairments in the water maze task and passive avoidance retention, but only weak and inconsistent effects on locomotor activity. These behavioural changes were paralleled by a reduction in choline acetyltransferase activity in hippocampus and several cortical areas (up to 97%) and selective depletions of NGF receptor-positive cholinergic neurons in the septal-diagonal band area and nucleus basalis magnocellularis (up to 99%). By contrast, the non-cholinergic parvalbumin-containing neurons in the septum were completely spared, and other cholinergic projection systems (such as in the striatum, thalamus, brainstem and spinal cord) were unaffected even at the highest dose. The observed changes in the water maze and passive avoidance tasks, as well as the cholinergic cell loss, were maintained up to at least 8 months following the intraventricular injection of a single dose (5 micrograms) of the immunotoxin. The results confirm the usefulness of the 192 IgG-saporin toxin for selective and profound lesions of the basal forebrain cholinergic neurons and provide further support for a role of the basal forebrain cholinergic system in cognitive functions.

Activation of metabotropic glutamate receptors protects cultured neurons against apoptosis induced by beta -amyloid peptide

Abstract: Prolonged exposure of cultured cortical cells or cultured cerebellar granule cells to the residue 25-35 fragment of beta-amyloid peptide (beta AP), beta AP(25-35), induced neuronal apoptosis, as revealed by morphological analysis, fluorescent chromatin staining, and immunodetection of oligonucleosomes released from the nucleus into the cytoplasm. beta AP(25-35)-induced apoptosis was insensitive to ionotropic glutamate receptor antagonists but was substantially attenuated by the metabotropic glutamate receptor (mGluR) agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid. The neuroprotective action of (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid was antagonized by (RS)-alpha-methyl-4-carboxyphenylglycine and was mimicked by (2S,1'R,2'R,3'R)-2-(2,3-dicarboxycyclopropyl)glycine (a selective agonist of mGluR2 and -3 subtypes) and by L-2-amino-4-phosphobutanoate and L-serine-O-phosphate (selective agonists of mGluR4, -6, and -7 subtypes). However, whereas all of these drugs behaved as neuroprotectants in cultured cortical cells, only L-2-amino-4-phosphobutanoate and L-serine-O-phosphate [and not (2S,1'R,2'R,3'R)-2-(2,3-dicarboxycyclopropyl)glycine] reduced beta AP(25-35)-induced apoptosis in cultured cerebellar granule cells. The neuroprotective activity of mGluR agonists may be related to their ability to inhibit membrane Ca2+ conductance, because drugs that block voltage-sensitive Ca2+ channels, such as nimodipine or Co2+, could also attenuate beta AP(25-35)-induced apoptosis.

Activation of Class II or III Metabotropic Glutamate Receptors Protects Cultured Cortical Neurons Against Excitotoxic Degeneration

Abstract: Trans-1-aminocyclopentane-1,3-dicarboxylic acid, a mixed agonist of all metabotropic glutamate receptor (mGluR) subtypes, is known to produce either neurotoxic or neuroprotective effects. We have therefore hypothesized that individual mGluR subtypes differentially affect neurodegenerative processes. Selective agonists of subtypes which belong to mGluR class II or III, such as (2s, 1′R,2′R,3′R)-2-(2,3-dicarboxycyclopropyl)-glycine (DCG-IV) (specific for subtypes mGluR2 or 3) or L-2-amino-4-phosphonobutanoate and L-serine-O-phosphate (specific for subtypes mGluR4, 6 or 7), were highly potent and efficacious in protecting cultured cortical neurons against toxicity induced by either a transient exposure to N-methyl-D-aspartate (NMDA) or a prolonged exposure to kainate. In contrast, agonists that preferentially activate class I mGluR subtypes (mGluR1 or 5), such as quisqualate or trans-azetidine-2,3-dicarboxylic acid, were inactive. DCG-IV was still neuroprotective when applied to cultures after the toxic pulse with NMDA. This delayed rescue effect was associated with a reduction in the release of endogenous glutamate, a process that contributes to the maturation of neuronal damage. We conclude that agonists of class II or III mGluRs are of potential interest in the experimental therapy of acute or chronic neurodegenerative disorders.

Release of mast-cell-derived mediators after endobronchial adenosine challenge in asthma.

Abstract: Preformed and newly generated mediators released from airway mast cells may playa role in adenosine-induced bronchoconstriction. To investigate the possible role of mast-cell-derived mediator release in mediating bronchoconstriction induced by adenosine 5′-monophosphate (AMP), we have examined the fluid obtained by bronchoalveolar lavage for inflammatory mediators and markers of airway permeability immediately after instillation of AMP directly into an airway segment of 10 asthmatic subjects. Eight subjects completed the protocol. When compared with the saline-challenged segment, the response to endobronchial stimulation with AMP was characterized by a prompt reduction in airway caliber paralleled by a significant rise in PGD2, histamine, and tryptase levels in the lavage fluid. After AMP challenge, the median (range) concentration for PGD2 increased from 36 to 205 pg/ml (p = 0.006), for histamine from 184 to 433 pg/ml (p = 0.018), and for tryptase from 0.30 to 0.54 ng/ml (p = 0.013). In addition, a small...

Overview of the Late Tertiary–Recent tectonic and palaeo-environmental development of the Mediterranean region

Abstract: The Late Tertiary history of the Mediterranean region exemplifies processes of ocean basin closure and continental collision, as determined from integrated land and marine evidence. During the Mesozoic–Early Tertiary, tectonic settings were dominated by evolution of Neotethys. This ocean generally widened eastwards, with a number of oceanic strands in the Eastern Mediterranean area. Great diversity of tectonic settings and palaeo-environments developed during the Tertiary closure history of these oceanic basins. In the Eastern Mediterranean region, more northerly Neotethyan strands were closed by the Mid Tertiary, while oceanic crust remained in the south in the present Eastern Mediterranean Sea area. Northwards subduction of the remaining southerly Neotethyan strand was probably active by the Early Miocene. Different areas exhibit different stages of convergence and ocean basin closure. In the east, the amalgamated Eurasian plate had collided with the Arabian margin (Africa) by the Late Miocene, while oceanic crust still persisted further west. Steady-state subduction during the Late Tertiary gave rise to the Mediterranean ridge, as a substantial mud-dominated accretionary wedge. In the Aegean area, sufficient northward subduction took place to activate arc volcanism and pervasive back arc extension, short of marginal basin opening. In the easternmost Mediterranean, only limited subduction took place, associated with supra-subduction zone extension (e.g. in Cyprus). Today, steady state-subduction continues only locally, where vestiges of Neotethys remain (e.g. Herodotus abyssal plain). In the Western Mediterranean area, suturing of the African and Eurasian plates initially took place in the Betic region (Early–Mid Tertiary), where the Neotethys had existed only as a narrow connection with the Central North Atlantic. In the Central Mediterranean region, where the Western Neotethys was wider, northward subduction was active, apparently as early as the Late Cretaceous. In a widely accepted interpretation, an Andean-type magmatic arc developed along the southern margin of Europe and was then rifted off in the Late Oligocene-Early Miocene, to form the Corsica-Sardinia Block, opening the North Balearic marginal basin in its wake. The migrating subduction zone and microcontinent then collided diachronously with North Africa-related continental units (North Africa and Apulia) from Late Oligocene-Early Miocene, giving rise to collisional thrust belts in the Northern and Southern Apennines and along the North African continental margin (i.e. the Maghrebian chain) to the Betic-Rif area. From the Early Miocene onwards, a separate subduction system became active, related to removal of Neotethyan oceanic crust to the southeast (Ionian Sea), fueling suprasubduction zone extension and opening of the Tyrrhenian Sea. ‘Orogenic collapse’ is an alternative mechanism of such extension, and is widely believed to have caused divergent thrusting in the Betic and Rif regions of the westernmost Mediterranean, at the same time as crustal extension and subsidence of the Alboran Sea.

Cooperative upconversion in erbium-implanted soda-lime silicate glass optical waveguides

Abstract: Soda-lime silicate glass has been locally doped with 0.2-at. % erbium by 3.0- and 5.0-MeV ion implantation. Single-mode fiber-compatible optical waveguides were then fabricated by use of Na+ ↔ K+ ion exchange. Characteristic photoluminescence (PL) of Er3+ centered at 1.54 μm is observed on excitation at 1.48 μm. For low pump intensity the PL decay is nearly single exponential with a lifetime of 7.2 ms. At high intensity it becomes nonexponential as a result of cooperative upconversion, an interaction between excited Er ions. Self-consistent modeling of the PL intensity and decay data yields an upconversion coefficient of 3.2 ± 0.8 × 10−24 m3/s. The effect of upconversion on optical gain is shown and discussed. An extrapolation of measured optical gain shows that 1 dB/cm of net gain is possible in the present Er-implanted soda-lime glass.

Improved hydrodynamical model for carrier transport in semiconductors.

Abstract: A set of closed hydrodynamiclike equations is derived from Boltzmann's transport equation (BTE) describing charge transport in semiconductors. The production terms are modeled as relaxation terms consistently with the Onsager reciprocity principle. Stationary and homogeneous solutions are explicitly treated. The form of the production terms is checked by applying the Grad method of moments to the BTE. Finally the model is compared with Monte Carlo simulations for silicon.

Patterns of Movement

Abstract: Motility is one of the most characteristic features of animals. Modification of the body for movement in different media represents the main course pursued by biological evolution throughout the animal kingdom. Locomotion allows animals to solve many environmental problems, from self-protection and nutrition to reproduction and social interactions. Movement may be continuous or discontinuous, periodic or aperiodic, individual or collective, small- or large-range, slow or fast. It may involve several mechanisms of orientation, and represents the basis of dispersal and the colonization of new territories.

Levothyroxine and Potassium Iodide Are Both Effective in Treating Benign Solitary Solid Cold Nodules of the Thyroid

Abstract: Objective: To determine the effectiveness of levothyroxine and potassium iodide in treating patients with benign solitary cold thyroid nodules Design: Randomized controlled study Setting: Outpati

Oblique rifting in Salina, Lipari and Vulcano islands (Aeolian islands, southern Italy)

Abstract: A structural analysis carried out on the volcanic products of the islands of Salina, Lipari and Vulcano (Aeolian archipelago) points out that the large-scale tectonic setting is dominated by NW-SE trending right-lateral extensional strike-slip faults and by N-S to NE-SW trending normal faults and fractures. This fault pattern generates pull-apart type structures, developing between different right-hand overlapping fault segments and a characteristic extensional imbricate fan geometry at the tip of the major strike-slip faults. All the structures, representing the surface expression of an active crustal discontinuity which controls the evolutionary history of the magmatism of the three islands, are kinematically compatible with a N100°E extension related to a rifting process affecting southern Italy.

Molecular weight distribution of poly(dimethylsiloxane) by combining matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with gel-permeation chromatography fractionation

Abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows detection of large molecules such as those present in synthetic and natural macromolecules. Until recently, it was reported that MALDI-TOF measurements can provide correct molecular weight (MW) estimates only for nearly monodisperse polymer samples. We have now developed a methodology for polydisperse samples. We recorded the gel-permeation chromatography (GPC) trace of a polydisperse polymeric sample of poly(dimethylsiloxane) (PDMS), collecting 42 fractions. Selected fractions were analyzed by MALDI-TOF and the average MW of each fraction was determined, allowing a calibration of GPC curves against absolute MW. The calibrated GPC trace was then used to compute average MW and molecular-weight distribution (MWD) of the unfractionated poly(dimethylsiloxane) sample. In the spectra of low molecular-weight fractions, the resolution is high enough to resolve the contributions of the various PDMS oligomers as separate signals.

Identification of in vivo and in vitro lemon mutants by RAPD markers.

Abstract: SummaryRandom Amplified Polymorphic DNA (RAPD) assay was performed for the identification of 14 in vivo and one in vitro lemon mutants in comparison with a known zygotic origin genotype. Thirty six 10-mer arbitrary primers were employed to detect RAPD markers. Fourteen of the tested primers generated sole monomorphic profiles and 22 produced 43 polymorphic fragments in a total of 294 loci. With the polymorphisms obtained in the present assay, all the 16 lemon genotypes were identified. Among the 43 RAPD markers, 28 were unique to a single genotype, of which 20 were specific to ‘Interdonato’, four to ‘Ruvittaro’, three to ‘Monachello’ and one to ‘Femminello Continella’. The results of the pairwise analysis of the RAPD data indicated that ‘Interdonato’ was genetically distinct from the other lemons, while high similarities were observed among the remaining genotypes. High reproducibility of RAPD technique was observed in the analysis of three DNA preps. The RAPDs obtained in the present study have provided ...

Childhood IQ measurements in infants with transient congenital hypothyroidism.

Abstract: Summary OBJECTIVE In view of the fact that, during the first period of life, thyroid hormones are critical for brain development, we investigated whether even transient congenital hypothyroidism could affect the long-term intellectual development of affected infants. DESIGN A case-control study of intellectual development, auxometric parameters and thyroid function performed in late infancy in children with documented transient congenital hypothyroidism or hyperthyrotrophinaemia at birth. PATIENTS Nine children born in an endemic goitre area who had short-term transient congenital hypothyroidism or hyperthyrotrophinaemia after birth (TCH) were studied and compared to nine matched children born in the same area at the same time but having normal thyroid function at birth (N). MEASUREMENTS Global, verbal and performance IQs were evaluated on the Wechsler scale. Height, bone age, total and free thyroid hormones, thyroid volume, thyroglobulin, basal and TRH stimulated TSH were also measured. RESULTS Height and bone age were similar in the two groups. Thyroid function tests were also similar in the two groups except for basal and TRH stimulated serum TSH and serum Tg which were higher in the TCH than in the control group. Global, verbal and performance IQs were systematically lower in the TCH than in the N group. (78.3 ± 11.1 vs 90.9 ± 14.2, P < 0.05; 84.4 ± 15.4 vs 96.2 ± 14.8, P NS; 75.0 ± 8.5 vs 89.2 ± 12.5, P < 0.01 respectively). CONCLUSION Infants born and living in an endemic goitre area, who had biochemical signs of thyroid hypofunction at birth, had a lower intelligence quotient at the age of 7–8 years than matched controls living in the same environmental conditions but with normal thyroid function at birth. The present findings strongly suggest that abnormalities in thyroid function at birth, even when transient, can adversely affect long-term intellectual development.

Tectonic role of ophiolite‐bearing terranes in the development of the Southern Apennines orogenic belt

Abstract: New data on ophiolite-bearing terranes of the Liguride Complex, together with some information on the terranes of the Sicilide Complex, result in a better understanding of the role and tectonic significance of these units in the construction of the Southern Apennines orogenic belt. The Liguride Complex is composed of two main tectonic units overlain by a thick turbiditic sequence of Late Oligocene-Middle Miocene age. The uppermost one (Frido Unit) is a polydeformed and polymetamorphosed sequence, composed of two tectonic subunits of shales and calc-schists, respectively, containing blocks of ophiolite, garnet gneiss, amphibolites and granitoids. This unit is thrust over the un-metamorphosed terranes (Calabro–Lucano Flysch Unit) consisting of a broken formation with blocks of Late Jurassic ophiolite and their sedimentary cover, Cretaceous-Eocene pelagic sediments and Late Oligocene volcaniclastic deposits. The Frido Unit underwent HP/LT metamorphism (P= 8–10 Kb; T= 400–500 °C) resulting in glaucophane and lawsonite assemblages in the ophiolitic rocks and aragonite in the meta-limestones and calc-schists, followed by greenschist fades metamorphism (P= 4 Kb; T= 300–350 °C). From a structural point of view units of the Liguride Complex comprise structures developed at different structural levels, indicating progressive non-coaxial deformation in response to tectonic transport towards the N-NE. The ophiolite-bearing terranes of the Liguride Complex can be considered as a remnant of an accretionary complex in which the Calabro Lucano Flysch Unit represents the toe of the wedge where frontal accretion processes occur and the Frido Unit is a deeper portion. Emplacement of the Frido Unit is explained as being due to formation of a deep duplex structure during the early stage of continental collision processes. The polarity of tectonic transport provides new evidence that the Liguride Complex represents a suture zone between the Apulian and the Calabrian blocks. The age of collision appears to be not older than late Oligocene. The allochtonous terranes of the Liguride and Sicilide Complexes, therefore, represent a complete accretionary wedge which records, first, subduction of the Neotethys ocean beneath the Calabrian (Europe) continental margin and, later, continental collision with the African block.

Disruption of hypothalamic-pituitary-adrenocortical system in transgenic mice expressing type II glucocorticoid receptor antisense ribonucleic acid permanently impairs T cell function: effects on T cell trafficking and T cell responsiveness during postnatal development

Abstract: We used transgenic mice with impaired corticosteroid receptor function, caused by expression of type II glucocorticoid receptor (GR) antisense RNA, to study the role of glucocorticoid feedback during the developmental maturation of hypothalamus-pituitary-adrenal-immune functions. These mice have increased plasma concentrations of ACTH and corticosterone as well as reduced GR binding capacity. In control mice, a strong sex dimorphism in the development of GR gene expression is apparent, and in males between postnatal days 9-36, the GR gene transcript concentration is approximately twice that in female mice. Endogenous GR messenger RNA levels were markedly reduced in transgenic mice, and the sex dimorphism was abolished. An abnormal developmental pattern of adrenal secretory activity accompanied the postnatal maturation of the hypothalamic-pituitary-adrenocortical system of the transgenic mice, and high plasma corticosterone levels were measured at early postnatal ages through adulthood. Inefficient glucoco...