University of New Brunswick

About: University of New Brunswick is a education organization based out in Fredericton, New Brunswick, Canada. It is known for research contribution in the topics: Population & Context (language use). The organization has 10498 authors who have published 20654 publications receiving 474448 citations.

Rapid Isolation of Extracellular Vesicles from Cell Culture and Biological Fluids Using a Synthetic Peptide with Specific Affinity for Heat Shock Proteins

Abstract: Recent studies indicate that extracellular vesicles are an important source material for many clinical applications, including minimally-invasive disease diagnosis. However, challenges for rapid and simple extracellular vesicle collection have hindered their application. We have developed and validated a novel class of peptides (which we named venceremin, or Vn) that exhibit nucleotide-independent specific affinity for canonical heat shock proteins. The Vn peptides were validated to specifically and efficiently capture HSP-containing extracellular vesicles from cell culture growth media, plasma, and urine by electron microscopy, atomic force microscopy, sequencing of nucleic acid cargo, proteomic profiling, immunoblotting, and nanoparticle tracking analysis. All of these analyses confirmed the material captured by the Vn peptides was comparable to those purified by the standard ultracentrifugation method. We show that the Vn peptides are a useful tool for the rapid isolation of extracellular vesicles using standard laboratory equipment. Moreover, the Vn peptides are adaptable to diverse platforms and therefore represent an excellent solution to the challenge of extracellular vesicle isolation for research and clinical applications.

System and method for image fusion

Abstract: A method of fusing a low resolution multispectral image with a high resolution panchromatic image comprising the step of generating a new synthetic pan image using multispectral bands from the multispectral image.

Behaviour during elevated water temperatures: can physiology explain movement of juvenile Atlantic salmon to cool water?

Abstract: Summary 1. Temperature governs most physiological processes in animals. Ectotherms behaviourally thermoregulate by selecting habitats with temperatures regulating their body temperature for optimal physiological functioning. However, ectotherms can experience temperature extremes forcing the organisms to seek temperature refuge. 2. Fish actively avoid potentially lethal temperatures by moving to cool-water sites created by inflowing tributaries and groundwater seeps. Juvenile Atlantic salmon (Salmo salar) of different age classes exhibit different behavioural responses to elevated temperatures (>23 °C). Yearling (1+) and 2-year-old (2+) Atlantic salmon often cease feeding, abandon territorial behaviour and swim continuously in aggregations in cool-water sites; whereas young-of-the-year (0+) fish continue defending territories and foraging. 3. This study determined whether the behavioural shift in older individuals (2+) occurred when basal metabolic rate, driven by increasing water temperature, reached the maximum metabolic rate such that anaerobic pathways were recruited to provide energy to support vital processes. Behaviour (feeding and stress responses), oxygen consumption, muscle lactate and glycogen, and circulating blood lactate and glucose concentrations were measured in wild 0+ and 2+ Atlantic salmon acclimated to water temperatures between 16 and 28 °C. 4. Results indicate that oxygen consumption of the 2+ fish increased with temperature and reached a plateau at 24 °C, a temperature that corresponded to cessation of feeding and a significant increase in muscle and blood lactate levels. By contrast, oxygen consumption in 0+ fish did not reach a plateau, feeding continued and muscle lactate did not increase, even at the highest temperatures tested (28 °C). 5. To conclude, the experiment demonstrated that the 0+ and 2+ fish had different physiological responses to the elevated water temperatures. The results suggest that wild 2+ Atlantic salmon employ behavioural responses (e.g. movement to cool-water sites) at elevated temperatures in an effort to mitigate physiological imbalances associated with an inability to support basal metabolism through aerobic metabolic processes.

Attitude Toward Self, Social Factors, and Achievement in Mathematics: A Meta-Analytic Review

Abstract: This meta-analysis integrated 143 primary studies on the relationship of attitude toward self and social factors with achievement in mathematics. Attitude was decomposed into self-concept about mathematics, perception of family support, and perception of mathematics as a male domain. Major findings included: (a) self-concept, family support, and mathematics as a male domain were all related to achievement; (b) the three relationships did not show significant gender differences; (c) the three relationships consistently decreased from the junior high grades to the senior high grades; (d) the relationship between self-concept and achievement varied as a function of ethnicity, whereas the relationship between family support and achievement was consistent across ethnic background; (e) the three relationships all varied across sample selection; (f) the relationship between self-concept and achievement varied with sample size, whereas the relationships of family support and mathematics as a male domain with achievement were sample-size invariant; (g) the relationship between self-concept and achievement increased over time, whereas the relationships of family support and mathematics as a male domain with achievement remained almost unchanged over time; and (h) there were no statistically significant interaction effects among gender, grade, and ethnicity for any of the three relationships.