Institution
University of York
Education•York, York, United Kingdom•
About: University of York is a education organization based out in York, York, United Kingdom. It is known for research contribution in the topics: Population & Health care. The organization has 22089 authors who have published 56925 publications receiving 2458285 citations. The organization is also known as: York University & Ebor..
Topics: Population, Health care, Context (language use), Randomized controlled trial, Cost effectiveness
Papers published on a yearly basis
Papers
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TL;DR: Glycosylation alters the hydrophilicity of the acceptors, their stability and chemical properties, their subcellular localisation and often their bioactivity, and the utility of GTs as biocatalysts is understood.
435 citations
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TL;DR: The Consolidated Health Economic Evaluation Reporting Standards (CHEERS) statement is an attempt to consolidate and update previous health economic evaluation guidelines efforts into one current, useful reporting guidance.
Abstract: Economic evaluations of health interventions pose a particular challenge for reporting. There is also a need to consolidate and update existing guidelines and promote their use in a user friendly manner. The Consolidated Health Economic Evaluation Reporting Standards (CHEERS) statement is an attempt to consolidate and update previous health economic evaluation guidelines efforts into one current, useful reporting guidance. The primary audiences for the CHEERS statement are researchers reporting economic evaluations and the editors and peer reviewers assessing them for publication. The need for new reporting guidance was identified by a survey of medical editors. A list of possible items based on a systematic review was created. A two round, modified Delphi panel consisting of representatives from academia, clinical practice, industry, government, and the editorial community was conducted. Out of 44 candidate items, 24 items and accompanying recommendations were developed. The recommendations are contained in a user friendly, 24 item checklist. A copy of the statement, accompanying checklist, and this report can be found on the ISPOR Health Economic Evaluations Publication Guidelines Task Force website (
http://www.ispor.org/TaskForces/EconomicPubGuidelines.asp
). We hope CHEERS will lead to better reporting, and ultimately, better health decisions. To facilitate dissemination and uptake, the CHEERS statement is being co-published across 10 health economics and medical journals. We encourage other journals and groups, to endorse CHEERS. The author team plans to review the checklist for an update in five years.
435 citations
01 Jan 1999
435 citations
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TL;DR: Several new methods are evaluated for use in the improvement of experimental phases in the framework of a classical density-modification calculation and have been implemented in a new computer program, Parrot.
Abstract: Classical density-modification techniques (as opposed to statistical approaches) offer a computationally cheap method for improving phase estimates in order to provide a good electron-density map for model building. The rise of statistical methods has lead to a shift in focus away from the classical approaches; as a result, some recent developments have not made their way into classical density-modification software. This paper describes the application of some recent techniques, including most importantly the use of prior phase information in the likelihood estimation of phase errors within a classical density-modification framework. The resulting software gives significantly better results than comparable classical methods, while remaining nearly two orders of magnitude faster than statistical methods.
434 citations
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TL;DR: The analysis shows that the environment of the catalytically active N-terminal serine of the B chain contains no adjacent histidine equivalent to that found in the serine proteases, indicating that this must be an important recognition site for cleavage.
Abstract: PENICILLIN acylase (penicillin amidohydrolase, EC 3.5.1.11) is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. It is used on an industrial scale for the production of 6-aminopenicillanic acid, the starting material for the synthesis of semi-synthetic penicillins. Its in vivo role remains unclear, however, and the observation that expression of the Escherichia coli enzyme in vivo is regulated by both temperature and phenylacetic acid has prompted speculation that the enzyme could be involved in the assimilation of aromatic compounds as carbon sources in the organism's free-living mode1. The mature E. coli enzyme is a periplasmic 80K heterodimer of A and B chains (209 and 566 amino acids, respectively2,3) synthesized as a single cytoplasmic precursor containing a 26-amino-acid signal sequence to direct export to the cytoplasm4 and a 54-amino-acid spacer between the A and B chains which may influence the final folding of the chains5. The N-terminal serine of the B chain reacts with phenylmethylsulphonyl fluoride, which is consistent with a catalytic role for the serine hydroxyl group. Modifying this serine to a cysteine6'7 inactivates the enzyme, whereas threonine, arginine or glycine substitution prevents in vivo processing of the enzyme7, indicating that this must be an important recognition site for cleavage. Here we report the crystal structure of penicillin acylase at 1.9 A resolution. Our analysis shows that the environment of the catalytically active N-terminal serine of the B chain contains no adjacent histidine equivalent to that found in the serine proteases. The nearest base to the hydroxyl of this serine is its own α-amino group, which may act by a new mechanism to endow the enzyme with its catalytic properties.
434 citations
Authors
Showing all 22432 results
Name | H-index | Papers | Citations |
---|---|---|---|
Cyrus Cooper | 204 | 1869 | 206782 |
Eric R. Kandel | 184 | 603 | 113560 |
Ian J. Deary | 166 | 1795 | 114161 |
Elio Riboli | 158 | 1136 | 110499 |
Claude Bouchard | 153 | 1076 | 115307 |
Robert Plomin | 151 | 1104 | 88588 |
Kevin J. Gaston | 150 | 750 | 85635 |
John R. Hodges | 149 | 812 | 82709 |
Myrna M. Weissman | 149 | 772 | 108259 |
Jeffrey A. Lieberman | 145 | 706 | 85306 |
Howard L. Weiner | 144 | 1047 | 91424 |
Dan J. Stein | 142 | 1727 | 132718 |
Jedd D. Wolchok | 140 | 713 | 123336 |
Bernard Henrissat | 139 | 593 | 100002 |
Joseph E. LeDoux | 139 | 478 | 91500 |