Showing papers in "Stem Cell Research in 2012"

TL;DR: The findings provide a better understanding of the mechanism(s) involved in stem cell aging and regenerative potential, and this in turn may affect tissue repair that declines with aging.

TL;DR: All five hiPS cell lines that were examined showed efficient differentiation into insulin-producing cells with this protocol, and several compounds were found to induce the differentiation of insulin- producing cells from pancreatic progenitor cells.

TL;DR: A scalable suspension culture system using serum-free defined media with spinner flasks for hESC expansion as cell aggregates provides a powerful approach for scale-up expansion of hESCs under defined and serum- free conditions for clinical and research applications.

TL;DR: Comparing the immunophenotype, gene expression, multipotency and immunosuppressive potential of MSC-like colony-forming cells from adult murine bone marrow, kidney and heart cultured under uniform conditions suggested organ-specific functions.

TL;DR: It is revealed that IGF-1 can promote the osteogenic differentiation and osteogenesis capacity of SCAPs, but weaken their odontogenic differentiate and dentinogenesis capability, indicating that IGF -1 treatedSCAPs can be used as a potential candidate for bone tissue engineering.

TL;DR: Low functionality of HLCs compared to primary human hepatocytes limits their usefulness in clinical practice, and novel techniques are needed to complete differentiation of hiPSCs to mature hepatocytes.

TL;DR: This study provides for the first time proof of concept that disease cell lines derived from skin cells obtained from an MS patient can be generated and successfully differentiated into mature neural lineages.

TL;DR: Under appropriate culture conditions, hHF-MSCs differentiated along the myogenic, osteogenic, adipogenic and chondrogenic lineages, as demonstrated by kinetic gene expression profiling and functional assays and Interestingly, the differentiation potential decreased with time in culture in a lineage-specific manner.

TL;DR: It is proved that both CMs and MSCs can be simultaneously generated from human induced pluripotent stem cells (hiPSCs) via a pro-mesoderm differentiation strategy in a low-cost and efficient one step manner.

TL;DR: Transplantation of BMMCs by IA or IV routes may lead to similar brain homing and therapeutic efficacy after experimental stroke, and functional recovery was observed in both groups up to 11 weeks after the lesion.

TL;DR: Findings indicate that apelin-13 is a survival factor for BMSCs and its anti-apoptotic property may prove to be of therapeutic significance in terms of exploiting BMSC-based transplantation therapy.

TL;DR: The potential of adult MSC to reduce brain damage under neurodegenerative diseases is highlighted and the use of nanoparticles in cell tracking is indicated, supporting their potential as valuable tools for cell therapy.

TL;DR: It is provided evidence to show that compressive load can improve the stiffness of collagen substrates and have substantial influence in the development of ocular surface constructs or experimental models particularly in the fields of stem cell research, tissue engineering and regenerative medicine.

TL;DR: It is concluded that DLK1(PREF1) is well expressed in human ASC and acts as a negative regulator of adipogenesis and could be a functional marker contributing to the characterization of human ASC.

TL;DR: Results indicate specific laminin isoforms and integrins in maintenance of hESC pluripotency in feeder-dependent cultures and several genes with a known or possible role for hESCs pluripOTency were differentially expressed in distinct feeder cells.

TL;DR: Eltrombopag enhanced expansion of HSCs/HPCs of human UCB in vivo and in vitro, and promoted multi-lineage hematopoiesis through the expansion of bone marrow HSCS/H PCs ofhuman UCb in vivo.

TL;DR: It is demonstrated that MMP-9 is preferentially expressed at the leading edge of limbal epithelial outgrowth, and the data reveal that PI3-K/Akt is an upstream component of the JNK1/2 pathway in M MP-9 expression.

TL;DR: It is shown that human hair follicle-derived iPSC can be differentiated into various neural lineages, suggesting this experimental system as a promising in vitro model to study normal and pathological neural developments, avoiding the invasiveness of commonly used skin biopsies.

TL;DR: An effect of taurine (1-10 mM) increasing markedly (120%) the number of neural precursor cells (NPCs) from adult mouse subventricular zone, cultured as neurospheres is reported, one of the highest reported for adult Neural precursor cells.

TL;DR: In conclusion, mesenchymal and myeloid fibroblast progenitors are multipotent, serve as progenitor cells in animal models, and are implicated in several diseases.

TL;DR: EFs are an effective signal that guides EPC migration through VEGF receptor signaling in vitro and may be used to control behaviors of EPCs in tissue engineering, in homing of E PCs to wounds and to an injury site in the vasculature.

TL;DR: The current knowledge on embryonic hematopoiesis is reviewed and the remaining questions regarding the anatomical and cellular identities of HSC precursors are highlighted.

TL;DR: Evidence is presented on how hypoxia selectively impacts the cellular response of BMSCs and ASCs, thus pointing for the need to optimize oxygen tension according to the cell source.

TL;DR: A novel cell culture method based on a non-colony type monolayer (NCM) growth has been shown to homogenize cellular states, precisely control growth rates, significantly increase cell production, and enhance hESC recovery from cryopreservation without compromising chromosomal integrity.

TL;DR: It is concluded that the Ascl3+ cells are intermediate lineage-restricted progenitor cells of the adult salivary glands, generating both duct and acinar cell types in vitro.

TL;DR: A model in which Bcl-xL promotes cell survival and increases cloning efficiency of dissociated hESCs without altering hESC self-renewal is supported by i) attenuation of apoptosis, and ii) upregulation of adhesion molecules to facilitate cell-cell or cell-matrix interactions.

TL;DR: The master regulatory gene Bmi1 modulates key stem cell properties in neural precursor cells (NPCs), and has been implicated in brain tumorigenesis, and expression profiles show a marked elevation in the proneural GBM subtype, and stem cell frequency as assessed by tumor sphere assays correlates with patient outcome.

TL;DR: Evidence is provided showing that GAG mimetics may have great interest for bone regeneration therapy and represent an alternative to exogenous growth factor treatments to optimize potential therapeutic properties of MSC.

TL;DR: It is reported that SKPs survive, respond to local cues, differentiate into myelinating Schwann cells, and avoid complete clearance by the host's immune defenses for a minimum of 10weeks.