Journal ArticleDOI
A fluorometric method for the differentiation of algal populations in vivo and in situ.
Martin Beutler,Karen Helen Wiltshire,Bettina Meyer,C. Moldaenke,C. Lüring,M. Meyerhöfer,Ulf-Peter Hansen,Holger Dau +7 more
TLDR
The method for algae differentiation described here opens up new research areas, monitoring and supervision tasks related to photosynthetic primary production in aquatic environments.Abstract:
Fingerprints of excitation spectra of chlorophyll (Chl) fluorescence can be used to differentiate 'spectral groups' of microalgae in vivo and in situ in, for example, vertical profiles within a few seconds. The investigated spectral groups of algae (green group, Chlorophyta; blue, Cyanobacteria; brown, Heterokontophyta, Haptophyta, Dinophyta; mixed, Cryptophyta) are each characterised by a specific composition of photosynthetic antenna pigments and, consequently, by a specific excitation spectrum of the Chl fluorescence. Particularly relevant are Chl a, Chl c, phycocyanobilin, phycoerythrobilin, fucoxanthin and peridinin. A laboratory-based instrument and a submersible instrument were constructed containing light-emitting diodes to excite Chl fluorescence in five distinct wavelength ranges. Norm spectra were determined for the four spectral algal groups (several species per group). Using these norm spectra and the actual five-point excitation spectrum of a water sample, a separate estimate of the respective Chl concentration is rapidly obtained for each algal group. The results of dilution experiments are presented. In vivo and in situ measurements are compared with results obtained by HPLC analysis. Depth profiles of the distribution of spectral algal groups taken over a time period of few seconds are shown. The method for algae differentiation described here opens up new research areas, monitoring and supervision tasks related to photosynthetic primary production in aquatic environments.read more
Citations
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Journal ArticleDOI
Fluorescence as a potential monitoring tool for recycled water systems: a review.
Rita K. Henderson,Andy Baker,Andy Baker,Kathleen R. Murphy,Kathleen R. Murphy,Adam Hambly,Richard M. Stuetz,Stuart J. Khan +7 more
TL;DR: It is concluded that the sensitive detection of contamination events in recycled water systems may be achieved by monitoring Peak T and/or Peak C fluorescence.
Journal ArticleDOI
Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues
Hazem M. Kalaji,Gert Schansker,Richard J. Ladle,Vasilij Goltsev,Karolina Bosa,Suleyman I. Allakhverdiev,Marian Brestic,Filippo Bussotti,Ángeles Calatayud,Piotr Dąbrowski,Nabil I. Elsheery,Lorenzo Ferroni,Lucia Guidi,Sander W. Hogewoning,Anjana Jajoo,Amarendra Narayan Misra,Sergio G. Nebauer,Simonetta Pancaldi,Consuelo Penella,DorothyBelle Poli,Martina Pollastrini,Zdzisława Romanowska-Duda,Beata Rutkowska,João Serôdio,K. Suresh,Wiesław Szulc,Eduardo Alberto Tambussi,Marcos Ezequiel Yanniccari,Marek Zivcak +28 more
TL;DR: Practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology is provided to scientists who have some experience with the application but are still in the process of discovering what it all means and how it can be used.
Journal ArticleDOI
Amazon River carbon dioxide outgassing fuelled by wetlands
Gwenaël Abril,Jean-Michel Martinez,L. Felipe Artigas,Patricia Moreira-Turcq,Marc F. Benedetti,Luciana O. Vidal,Tarik Meziane,Jung-Hyun Kim,Marcelo Bernardes,Nicolas Savoye,Jonathan Deborde,Edivaldo Lima Souza,Patrick Albéric,Marcelo Friederichs Landim de Souza,Fábio Roland +14 more
TL;DR: Wetland carbon export is potentially large enough to account for at least the 0.21 petagrams of carbon emitted per year as CO2 from the central Amazon River and its floodplains, suggesting a substantial fraction of CO2 evasion from inland waters.
Journal ArticleDOI
Freshwater phytoplankton quantification by chlorophyll a: a comparative study of in vitro, in vivo and in situ methods.
Jakub Gregor,Blahoslav Maršálek +1 more
TL;DR: Compared methods for chlorophyll a quantification, spectrofluorometry, and a submersible fluorescence probe for in situ phytoplankton quantification were compared in different freshwater environments-reservoirs and rivers.
Journal ArticleDOI
Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia.
TL;DR: A DNA-based molecular phylogenetic methods that do not require cultivation are used to study the microbial diversity of an irregular stromatolite and of the surface and interior of a domal strom atolite to provide a framework for understanding the kinds of organisms that build contemporary stromAtolites, their ecology, and their relevance to stromats preserved in the geological record.
References
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Book
Algae: An Introduction to Phycology
David G. Mann,H.M Jahns +1 more
TL;DR: In this article, the main groups of algae (divisions or phyla) are considered in turn, and the final chapter is a synthesis, in which the phylogeny of the algae is discussed in relation to the evolution of other living organisms.
Journal ArticleDOI
Yellow‐green algae with chlorophyllide c1,2
TL;DR: Chlorophyllide c (chlorophyll c) wax found in axenic or unialgal cultures of 5 members of the class Xanthophyceae and in 2 members ofThe class Raphidophyceai (ChloromonadophyceAE).
Journal ArticleDOI
Sixty-Three Years Since Kautsky: Chlorophyll a Fluorescence
TL;DR: Chlorophyll a fluorescence will remain as the one-most powerful tool for probing excitation energy transfer, primary photochemistry, electron flow on both the donor and the acceptor side of photosystem II (PSII) of oxygenic PSII.
Journal ArticleDOI
The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography
TL;DR: In this paper, a reverse-phase high-performance liquid chromatographic (h.p.l.c.) system is developed for a rapid separation and quantification of fourteen chlorophylls and their breakdown products and seventeen carotenoids from acetone extracts of algal cultures and natural waters.
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Freshwater phytoplankton quantification by chlorophyll a: a comparative study of in vitro, in vivo and in situ methods.
Jakub Gregor,Blahoslav Maršálek +1 more