Journal ArticleDOI
A general approach to DNA-programmable atom equivalents
Chuan Zhang,Robert J. Macfarlane,Kaylie L. Young,Chung Hang Jonathan Choi,Liangliang Hao,Evelyn Auyeung,Guoliang Liu,Xiaozhu Zhou,Chad A. Mirkin +8 more
TLDR
By coating nanoparticles protected with aliphatic ligands with an azide-bearing amphiphilic polymer, followed by the coupling of DNA to the polymer using strain-promoted azide -alkyne cycloaddition, nanoparticles bearing a high-density shell of nucleic acids can be created regardless of nanoparticle composition.Abstract:
Nanoparticles can be combined with nucleic acids to programme the formation of three-dimensional colloidal crystals where the particles' size, shape, composition and position can be independently controlled. However, the diversity of the types of material that can be used is limited by the lack of a general method for preparing the basic DNA-functionalized building blocks needed to bond nanoparticles of different chemical compositions into lattices in a controllable manner. Here we show that by coating nanoparticles protected with aliphatic ligands with an azide-bearing amphiphilic polymer, followed by the coupling of DNA to the polymer using strain-promoted azide-alkyne cycloaddition (also known as copper-free azide-alkyne click chemistry), nanoparticles bearing a high-density shell of nucleic acids can be created regardless of nanoparticle composition. This method provides a route to a virtually endless class of programmable atom equivalents for DNA-based colloidal crystallization.read more
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Clathrates grow up
Dipak Samanta,Rafal Klajn +1 more
TL;DR: Lin et al. (4) show that bipyramidal gold nanocrystals can be programmed to assemble into colloidal crystals with structures that resemble those of molecular clathrates, but on a much higher length scale.
Assembly of optically-active nanocomponents with DNA
Abstract: DNA-based self-assembly offers high accuracy and robustness to arrange optically active nanocomponents into complex photonic structures. Nanocomponents of different compositions, sizes, and shapes can be assembled into defined geometries with controlled orientations and separations. Investigating the properties of self-assembled photonic nanostructures can provide new insights into the physical mechanisms of the light-matter interactions or even reveal new phenomena. In this thesis, I demonstrate the placement of optically-active nanocomponents on DNA templates, from individual DNA structures to more complex three dimensional DNA matrices. Nanodiamonds (with enclosed nitrogen-vacancy color centers), colloidal quantum dots, and gold nanoparticles were assembled into different configurations such as homodimers, heterodimers, heterotrimers, and other hybrid structures. To achieve this, I developed new and optimized existing efficient functionalization methods which result in high binding accuracy and at the same time preserve the optical properties of the optical components. To spatially arrange nanoparticles in regular patterns in three dimensions, a triangular DNA origami structure was designed that can polymerize into a rhombohedral lattices of tens of micrometers in size. The large unit cell of 1.8 × 105 nm3 allows co-crystalization of large guest components. Electron microscopy and small-angle X-ray scattering confirmed the crystalline assembly of DNA origamis and the successful incorporation of gold nanoparticles of various sizes. Overall, DNA-templated assembly provides new opportunities to operate optical components in the nano-realm. Further improved assembly quality and complexity will allow to systematically study energy transfer events occurring in nanophotonic networks and will potentially lead to applications in energy harvesting devices and active plasmonics circuits.
Journal ArticleDOI
Molecularly pure miktoarm spherical nucleic acids: preparation and usage as a scaffold for abiotic intracellular catalysis
Bohan Zhang,Silei Bai,Xiangyu Chao,Tong Wu,Zhiyong Chen,Zehong Cheng,Yue Xiao,Ke Zhang,Ke Zhang,Yugang Bai +9 more
TL;DR: In this paper, a fullerene-based strategy was proposed for the synthesis of molecularly pure miktoarm spherical nucleic acids (SNAs) with diverse structures, which, with post-functionalization, could serve as efficient scaffolds for intracellular catalysis.
DNA-mediated assembly of optical components for light manipulation at the nanoscale
TL;DR: In this paper, a bottom-up-herstellungstechnik is used for DNA-Nanostrukturen, e.g., in the context of the Licht-Materie-Wechselwirkung.
References
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Journal ArticleDOI
Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology.
TL;DR: A review of gold nanoparticles can be found in this article, where the most stable metal nanoparticles, called gold colloids (AuNPs), have been used for catalysis and biology applications.
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Semiconductor Clusters, Nanocrystals, and Quantum Dots
TL;DR: In this article, the authors focus on the properties of quantum dots and their ability to join the dots into complex assemblies creates many opportunities for scientific discovery, such as the ability of joining the dots to complex assemblies.
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A DNA-based Method for Rationally Assembling Nanoparticles Into Macroscopic Materials
TL;DR: A method for assembling colloidal gold nanoparticles rationally and reversibly into macroscopic aggregates by using the specificity of DNA interactions to direct the interactions between particles of different size and composition is described.
Journal ArticleDOI
In vivo cancer targeting and imaging with semiconductor quantum dots
TL;DR: Sensitive and multicolor fluorescence imaging of cancer cells under in vivo conditions are achieved and a whole-body macro-illumination system with wavelength-resolved spectral imaging is integrated for efficient background removal and precise delineation of weak spectral signatures.
Journal ArticleDOI
Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles
TL;DR: A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported, which can detect about 10 femtomoles of an oligonucleotide.