Journal ArticleDOI
Agrocybe cylindracea lectin is a member of the galectin family.
TLDR
The complete amino acid sequence of Agrocybe cylindracea lectin was determined from the peptides obtained by chemical cleavages and enzymatic hydrolyses and several insertions suggest that β-strands S2, F3, and S4 and the loop structures between β-Strands F2 & S3 and F5 & S2 are different from those of galectins reported so far.Abstract:
The complete amino acid sequence of Agrocybe cylindracea lectin was determined from the peptides obtained by chemical cleavages and enzymatic hydrolyses. The sequence shows 19.1% and 36.8% identity with those of human galectin-1 and Coprinus lectin-1, a fungal galectin, respectively. Seven residues, which are commonly found in carbohydrate recognizing domain (CRD) of galectins, were conserved. However, several insertions in the sequence, compared with those of human galectin-1 and Coprinus lectin-1, suggest that β-strands S2, F3, and S4 and the loop structures between β-strands F2 & S3 and F5 & S2 are different from those of galectins reported so far.read more
Citations
More filters
Journal ArticleDOI
Molecular character of the recombinant antitumor lectin from the edible mushroom Agrocybe aegerita
TL;DR: The results reveal that AAL is a member of the galectin family and the dimeric form is the active unit for the functional performance and the molecular characters revealed by this study are significant for the in-depth investigation of the functional mechanism of this interesting protein.
Journal ArticleDOI
Lectin Engineering, a Molecular Evolutionary Approach to Expanding the Lectin Utilities
TL;DR: The current state of the art of lectin engineering is summarized and recent technological advances in this field including selection of template lectin, construction of a mutagenesis library, and high-throughput screening methods are discussed.
Journal ArticleDOI
The Galβ-(syn)-gauche configuration is required for galectin-recognition disaccharides
Jun Iwaki,Hiroaki Tateno,Nozomu Nishi,Toshikazu Minamisawa,Sachiko Nakamura-Tsuruta,Yoko Itakura,Junko Kominami,Tadasu Urashima,Takanori Nakamura,Jun Hirabayashi +9 more
TL;DR: The present attempt should provide a basis to solve the riddle of the glyco-code as well as to develop therapeutic inhibitors mimicking galectin ligands.
Book ChapterDOI
Fungal lectins: a growing family.
TL;DR: This chapter provides an overview of the approximately 80 types of mushroom and fungal lectins that have been isolated and studied to date and their properties, sugar-binding specificities, structural grouping into families, and applications for biological research being described.
References
More filters
Journal ArticleDOI
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
H. Schägger,G. von Jagow +1 more
TL;DR: A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described, and the omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.
Journal ArticleDOI
Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
TL;DR: Small amounts of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride membranes, stained with Coomassie Blue, and sequenced directly, suggesting that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.
Journal ArticleDOI
Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.
TL;DR: All amino acids, including proline, are converted quantitatively to phenylthiocarbamyl compounds and these are stable enough to eliminate any need for in-line derivatization, providing results comparable in sensitivity and precision to those obtained by state-of-the-art ion-exchange analyzers.
Journal ArticleDOI
The family of metazoan metal-independent β-galactoside-binding lectins: structure, function and molecular evolution
Jun Hirabayashi,Ken-ichi Kasai +1 more
TL;DR: It appears that beta-galactoside-binding lectins and some non-lectin proteins form a superfamily whose members are widely distributed from vertebrates to invertebrates and a consideration of molecular evolution suggests that lectins belonging to this family probably existed in the Precambrian era.
Journal ArticleDOI
Galectins: A Family of Animal Lectins That Decipher Glycocodes
Ken-ichi Kasai,Jun Hirabayashi +1 more
TL;DR: Crystallographic studies revealed that galectins and legume lectins such as concanavalin A have a common topology in spite of the absence of sequence homology, which suggests a possible relationship between animal and plant lectins, and the existence of a lectin super family.