Fruiting body development in Coprinus cinereus: regulated expression of two galectins secreted by a non-classical pathway.
TLDR
Heterologous expression of Cgl2 in Saccharomyces cerevisiae indicated that secretion of this protein occurred independently of the classical secretory pathway, and two fungal galectins, Cgl1 and cgl2, are differentially regulated during fruiting body formation.Abstract:
Fruiting body formation in the basidiomycete Coprinus cinereus is a developmental process that occurs as a response of the mycelium to external stimuli. First, localized, highly branched hyphal structures (knots) are formed as a reaction to nutritional depletion. Hyphal-knot formation is repressed by light; however, light signals are essential for the development of the hyphal knot into an embryonic fruiting body (primordium) as well as karyogamy, meiosis and fruiting body maturation. The role of the different environmental signals in the initial phases of fruiting body development was analysed. It was observed that two fungal galectins, Cgl1 and Cgl2, are differentially regulated during fruiting body formation. cgl2 expression initiated in early stages of fruiting body development (hyphal knot formation) and was maintained until maturation of the fruiting body, whereas cgl1 was specifically expressed in primordia and mature fruiting bodies. Immunofluorescence and immuno-electron microscopy studies detected galectins within specific fruiting body tissues. They localized in the extracellular matrix and the cell wall but also in membrane-bound bodies in the cytoplasm. Heterologous expression of Cgl2 in Saccharomyces cerevisiae indicated that secretion of this protein occurred independently of the classical secretory pathway.read more
Citations
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Galectinomics: finding themes in complexity.
TL;DR: Although the identification of many novel galectin relatives in widely divergent organisms has added significantly to the size and complexity of this intriguing protein family, several common themes arise, which suggest promising new research targets.
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Fruiting body production in Basidiomycetes.
Ursula Kües,Y. Liu +1 more
TL;DR: A better understanding of the developmental processes underlying fruiting in these model organisms is expected to help mushroom cultivation of other basidiomycetes in the future.
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Identification of genes in the bW/bE regulatory cascade in Ustilago maydis
TL;DR: It is demonstrated that in one of the early upregulated genes, the promoter can be bound by a bW/bE fusion protein in vitro, suggesting a connection to the mating process.
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The laccase multi-gene family in Coprinopsis cinerea has seventeen different members that divide into two distinct subfamilies.
TL;DR: Overexpression of the 17 non-allelic laccase genes under the control of a constitutive promoter identified nine active enzymes from subfamily 1, and Lcc15 is expected to be non-functional in lacc enzyme activity due to an internal deletion of about 150 amino acids.
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