An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes
Maria C. R. Medeiros,Ana L. G. Mestre,Pedro M. C. Inácio,Sanaz Asgarif,Inês M. Araújo,Peter C. Hubbard,Zélia Velez,M. Leonor Cancela,Paulo R. F. Rocha,Dago M. de Leeuw,Fabio Biscarini,Henrique L. Gomes +11 more
TLDR
An electrical method to measure extracellular bioelectrical activity in vitro using the Helmholtz capacitive double-layer established at the electrode surface that is an ideal tool to measure in vitro slow and temporally synchronized events that are often involved in long range intracellular signaling.Abstract:
An electrical method to measure extracellular bioelectrical activity in vitro is presented. This method exploits the Helmholtz capacitive double-layer established at the electrode surface. Small extracellular voltage variations in the order of μVs induce through the double-layer capacitor a displacement current that is measured. This current is then enhanced by a gain factor proportional to the electrode capacitance. In addition, when measurements are carried out at low frequencies in current mode the electrode contribution to the noise can be minimized. The performance of the electrodes and the method is demonstrated using zebrafish hearts and glioma cell cultures. We propose that this electrical method is an ideal tool to measure in vitro slow and temporally synchronized events that are often involved in long range intracellular signaling.read more
Citations
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Extracellular electrical recording of pH-triggered bursts in C6 glioma cell populations
Paulo R. F. Rocha,Maria C. R. Medeiros,Ulrike Kintzel,Johannes Vogt,Inês M. Araújo,Ana L. G. Mestre,Volker Mailänder,Volker Mailänder,Paul Schlett,Melanie Dröge,Leonid Schneider,Fabio Biscarini,Dago M. de Leeuw,Dago M. de Leeuw,Henrique L. Gomes +14 more
TL;DR: This model of pH-triggered bursting was unambiguously supported by deliberate, external acidification of the cell culture medium and was likely to directly perturb, in vivo, the functionality of the healthy neuronal network in the vicinity of the tumor bulk and may contribute to seizures in glioma patients.
Journal ArticleDOI
Human Breast Cancer Cells Demonstrate Electrical Excitability.
Mafalda Ribeiro,Aya Elghajiji,Scott P. Fraser,Zoë D. Burke,David Tosh,Mustafa B.A. Djamgoz,Paulo R. F. Rocha +6 more
TL;DR: Non-invasive in vitro electrical recordings of strongly metastatic MDA-MB-231 and weakly/non-metastatic MCF-7 breast cancer cell lines are presented, suggesting a link between the cells’ bioelectricity and invasiveness.
Journal ArticleDOI
Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
Miguel Cabello,Haobo Ge,Carmen Aracil,Despina Moschou,Pedro Estrela,Jose M. Quero,Sofia I. Pascu,Paulo R. F. Rocha +7 more
TL;DR: This experimental work suggests that Ca2⁺ is significantly affecting the electrophysiological communication pattern among PC-3 cell populations, and opens up new avenues for real time and highly sensitive investigations of prostate cancer signalling pathways.
Journal ArticleDOI
Bioelectrical Signal Detection Using Conducting Polymer Electrodes and the Displacement Current Method
Pedro M. C. Inácio,Ana L. G. Mestre,Maria C. R. Medeiros,Sanaz Asgarifar,Youssef Elamine,Joana Canudo,João M.A. Santos,José Bragança,Jorge Morgado,Fabio Biscarini,Henrique L. Gomes +10 more
TL;DR: In this article, the displacement current method combined with high capacitive polymer-based electrodes is proposed to measure clusters of cells and whole organs, which has great potential in fundamental studies of drug discovery and safety pharmacology.
Journal ArticleDOI
Ultra-low noise PEDOT:PSS electrodes on bacterial cellulose: A sensor to access bioelectrical signals in non-electrogenic cells
Pedro M. C. Inácio,Maria C. R. Medeiros,Tiago Carvalho,Rute C. Félix,Ana L. G. Mestre,Peter C. Hubbard,Quirina Ferreira,Jorge Morgado,Ana Charas,Carmen S. R. Freire,Fabio Biscarini,Fabio Biscarini,Deborah M. Power,Henrique L. Gomes +13 more
TL;DR: In this article, the authors demonstrate that the coating of cellulose nanofibers with conducting PEDOT:PSS ink allows the fabrication of a nanostructured surface that establishes a low electrical double-layer resistance with liquid solutions.
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