Journal ArticleDOI
Antioxidant rich Morus alba leaf extract induces apoptosis in human colon and breast cancer cells by the downregulation of nitric oxide produced by inducible nitric oxide synthase.
TLDR
This is the first report showing the downregulation of iNOS and induction of apoptosis by M. alba extract, capable of inducing cytotoxicity in human colon cancer and breast cancer cells.Abstract:
Morus species had been used widely in the traditional medicines for various diseases. In this study we report the in vitro antiproliferative activity of the methanol extract of Morus alba. The extract is capable of inducing cytotoxicity in human colon cancer (HCT-15) cells (IC(50) = 13.8 μg/ml) and breast cancer (MCF-7) cells (IC(50) = 9.2 μg/ml), resulted in significant morphological changes of the cells, fragmentation of DNA, and caspase-3 activation- characteristics of apoptosis. It downregulated the amount of nitric oxide (NO) produced as a result of inducible nitric oxide (iNOS) activation. The HPLC analysis of the extract showed epicatechin (20%), myricetin (10%), quercetin hydrate (12%), luteolin (12%), and kaempferol (6%) as the major active components and ascorbic acid, gallic acid, pelargonidine, and p-coumaric acid as the minor components. To the best of our knowledge, this is the first report showing the downregulation of iNOS and induction of apoptosis by M. alba extract.read more
Citations
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Journal ArticleDOI
Kaempferol: A Key Emphasis to Its Anticancer Potential
Muhammad Imran,Bahare Salehi,Javad Sharifi-Rad,Tanweer Aslam Gondal,Farhan Saeed,Ali Imran,Muhammad Shahbaz,Patrick Valere Tsouh Fokou,Muhammad Umair Arshad,Haroon Khan,Susana G. Guerreiro,Natália Martins,Leticia M. Estevinho +12 more
TL;DR: Even though growing evidence supports the use of kaempferol for cancer prevention, further preclinical and clinical investigations using kaEMPferol or kaempFERol-rich foods are of pivotal importance before any public health recommendation or formulation using kaempfol.
Journal ArticleDOI
New potential phytotherapeutics obtained from white mulberry (Morus alba L.) leaves.
TL;DR: The present work demonstrates the profound and unique phyto-pharmacological and nutritional profile of white mulberry leaves which containing considerable amounts of easy digestive proteins, carbohydrates, micro- and macronutrients, polyphenols, free amino acids, organic acids.
Journal ArticleDOI
Medicinal Plants in the Prevention and Treatment of Colon Cancer.
Paola Aiello,Maedeh Sharghi,Shabnam Malekpour Mansourkhani,Azam Pourabbasi Ardekan,Leila Jouybari,Nahid Daraei,Khadijeh Peiro,Sima Mohamadian,Mahdiyeh Rezaei,Mahdi Heidari,Ilaria Peluso,Fereshteh Ghorat,Anupam Bishayee,Wesam Kooti +13 more
TL;DR: The results indicate that grape, soybean, green tea, garlic, olive, and pomegranate are the most effective plants against colon cancer.
Journal ArticleDOI
Role of dietary bioactive natural products in estrogen receptor-positive breast cancer.
TL;DR: This review summarizes the role of bioactive natural products that are involved in the prevention and treatment of estrogen-related and ER-positive breast cancer.
Journal ArticleDOI
Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry
TL;DR: An ultra high performance liquid chromatography coupled with a triple quadrupole mass detection and electrospray ionization mass spectrometry method has been established for the simultaneous determination of the 14 nucleosides and nucleobases, 24 amino acids and two main alkaloids in mulberry leaf.
References
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Journal Article
Evaluation of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug Sensitivity in Culture Using Human and Other Tumor Cell Lines
Dominic A. Scudiero,Robert H. Shoemaker,Kenneth D. Paull,Anne Monks,Siobhan Tierney,Thomas H. Nofziger,Michael J. Currens,Donna Seniff,Michael R. Boyd +8 more
TL;DR: The new XTT reagent provides for a simplified, in vitro cell growth assay with possible applicability to a variety of problems in cellular pharmacology and biology, but still shares many of the limitations and potential pitfalls of MTT or other tetrazolium-based assays.
Book ChapterDOI
Apoptosis: Mechanisms and Roles in Pathology
Mark J. Arends,Andrew H. Wyllie +1 more
TL;DR: This chapter discusses the mechanisms and roles of apoptosis in pathology, and the arrival of neutrophil polymorphs permits digestion and phagocytosis of the constituents of the necrotic cells, but brings with it the risk of further tissue damage.
Journal ArticleDOI
Nitric oxide synthase activity in human breast cancer.
TL;DR: Calcium-dependent and -independent NO synthase activity was high in invasive tumours compared with benign or normal tissue, and for invasive ductal carcinomas, NO biosynthesis was significantly greater for grade III compared with grade II tumours.
Journal Article
Frequent Nitric Oxide Synthase-2 Expression in Human Colon Adenomas: Implication for Tumor Angiogenesis and Colon Cancer Progression
Stefan Ambs,William G. Merriam,William P. Bennett,Emanuela Felley-Bosco,Mofolusara O. Ogunfusika,S. M. Oser,Shawn Klein,Peter G. Shields,Timothy R. Billiar,Curtis C. Harris +9 more
TL;DR: Data from this study are consistent with the hypothesis that excessive NO production by NOS2 may contribute to the pathogenesis of colon cancer progression at the transition of colon adenoma to carcinoma in situ.