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Open AccessJournal ArticleDOI

Atomic Force Microscopy, a Powerful Tool in Microbiology

Yves F. Dufrêne
- 01 Oct 2002 - 
- Vol. 184, Iss: 19, pp 5205-5213
TLDR
Electron microscopy has long been recognized as a key technique in microbiology to elucidate cell surface ultrastructure and this work's achievement is an exciting achievement.
Abstract
Understanding the functions of microbial cell surfaces requires knowledge of their structural and physical properties. Electron microscopy has long been recognized as a key technique in microbiology to elucidate cell surface ultrastructure ([6][1], [7][2], [44][3], [45][4]). An exciting achievement

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Citations
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Nanomechanical analysis of cells from cancer patients

TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI

Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions.

TL;DR: The theories that are reviewed are the Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, the thermodynamic approach and the extended DLVO theory, which provide the basis for theoretical analysis of the receptor-ligand interactions.
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Nanoscale mapping of the elasticity of microbial cells by atomic force microscopy

TL;DR: This is the first report in which spatially resolved AFM force curves are used to distinguish regions of different elasticity at the surface of single microbial cells in relation with function (i.e., cell division), and this approach will provide fundamental insights into the spatial distribution of physical properties at heterogeneous microbial cell surfaces.
Journal ArticleDOI

Polymeric nanoparticles: A study on the preparation variables and characterization methods

TL;DR: This review focuses on the toolbox of available methods for the preparation of polymeric nanoparticles and highlights some recent examples from the literature that demonstrate the influence of the preparation method on the physicochemical characteristics of the nanoparticles.
References
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Journal ArticleDOI

Atomic force microscope

TL;DR: The atomic force microscope as mentioned in this paper is a combination of the principles of the scanning tunneling microscope and the stylus profilometer, which was proposed as a method to measure forces as small as 10-18 N. As one application for this concept, they introduce a new type of microscope capable of investigating surfaces of insulators on an atomic scale.
Journal ArticleDOI

Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles

TL;DR: Gram-negative cell walls are strong enough to withstand ;3 atm of turgor pressure, tough enough to endure extreme temperatures and pHs, and elastic enough to be capable of expanding several times their normal surface area.
Journal ArticleDOI

Detection and localization of individual antibody-antigen recognition events by atomic force microscopy

TL;DR: It is indicated that this methodology has promise for characterizing rate constants and kinetics of molecular recognition complexes and for molecular mapping of biosurfaces such as membranes.
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