Journal ArticleDOI
Automated segmentation of electron tomograms for a quantitative description of actin filament networks.
Alexander Rigort,David Günther,Reiner Hegerl,Daniel Baum,Britta Weber,Steffen Prohaska,Ohad Medalia,Ohad Medalia,Wolfgang Baumeister,Hans-Christian Hege +9 more
TLDR
An automated procedure for the segmentation of actin filaments is described, which combines template matching with a new tracing algorithm, and the result is a set of lines, each one representing the central line of a filament.About:
This article is published in Journal of Structural Biology.The article was published on 2012-01-01. It has received 185 citations till now. The article focuses on the topics: Protein filament.read more
Citations
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Journal ArticleDOI
Visualizing the molecular sociology at the HeLa cell nuclear periphery
Julia Mahamid,Stefan Pfeffer,Miroslava Schaffer,Elizabeth Villa,Radostin Danev,Luis Kuhn Cuellar,Friedrich Förster,Anthony A. Hyman,Jürgen M. Plitzko,Wolfgang Baumeister +9 more
TL;DR: Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ, which revealed the native structure and organization of the cytoplasmic translation machinery.
Journal ArticleDOI
Cryo-electron tomography: The challenge of doing structural biology in situ
TL;DR: Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure, and recent technical advances have enabled the extraction of high fidelity structural information.
Journal ArticleDOI
Convolutional neural networks for automated annotation of cellular cryo-electron tomograms
Muyuan Chen,Wei Dai,Stella Y. Sun,Darius Jonasch,Cynthia Y. He,Michael F. Schmid,Wah Chiu,Steven J. Ludtke +7 more
TL;DR: A method that uses neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction for cellular electron cryotomography is introduced.
Journal ArticleDOI
In Situ Architecture and Cellular Interactions of PolyQ Inclusions
Felix J.B. Bäuerlein,Itika Saha,Archana Mishra,Maria Kalemanov,Antonio Martinez-Sanchez,Rüdiger Klein,Irina Dudanova,Mark S. Hipp,F. Ulrich Hartl,Wolfgang Baumeister,Rubén Fernández-Busnadiego +10 more
TL;DR: Advanced cryo-electron tomography methods suggest that aberrant interactions between fibrils and endomembranes contribute to the deleterious cellular effects of protein aggregation.
Journal ArticleDOI
In Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology.
TL;DR: A detailed computational workflow for in situ structural analysis is outlined and two recent studies are described to illustrate how this workflow can be adapted to examine both known and unknown cellular complexes.
References
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Book
Introduction to Algorithms
TL;DR: The updated new edition of the classic Introduction to Algorithms is intended primarily for use in undergraduate or graduate courses in algorithms or data structures and presents a rich variety of algorithms and covers them in considerable depth while making their design and analysis accessible to all levels of readers.
Journal ArticleDOI
Cell migration: integrating signals from front to back.
Anne J. Ridley,Martin A. Schwartz,Keith Burridge,Richard A. Firtel,Mark H. Ginsberg,Gary G. Borisy,J. Thomas Parsons,Alan Rick Horwitz +7 more
TL;DR: The mechanisms underlying the major steps of migration and the signaling pathways that regulate them are described, and recent advances investigating the nature of polarity in migrating cells and the pathways that establish it are outlined.
Journal ArticleDOI
Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
Journal ArticleDOI
A Review of Image Denoising Algorithms, with a New One
TL;DR: A general mathematical and experimental methodology to compare and classify classical image denoising algorithms and a nonlocal means (NL-means) algorithm addressing the preservation of structure in a digital image are defined.
Journal ArticleDOI
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Thomas D. Pollard,Gary G. Borisy +1 more
TL;DR: A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion.