Cannabinoid receptor localization in brain
TLDR
The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in the in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience.Abstract:
[3H]CP 55,940, a radiolabeled synthetic cannabinoid, which is 10-100 times more potent in vivo than delta 9-tetrahydrocannabinol, was used to characterize and localize a specific cannabinoid receptor in brain sections. The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in our in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience. Autoradiography of cannabinoid receptors in brain sections from several mammalian species, including human, reveals a unique and conserved distribution; binding is most dense in outflow nuclei of the basal ganglia--the substantia nigra pars reticulata and globus pallidus--and in the hippocampus and cerebellum. Generally high densities in forebrain and cerebellum implicate roles for cannabinoids in cognition and movement. Sparse densities in lower brainstem areas controlling cardiovascular and respiratory functions may explain why high doses of delta 9-tetrahydrocannabinol are not lethal.read more
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Chronic exposure to delta 9-tetrahydrocannabinol fails to irreversibly alter brain cannabinoid receptors.
Tracy M. Westlake,Allyn C. Howlett,Syed F. Ali,Merle G. Paule,Andrew C. Scallet,William Slikker +5 more
TL;DR: It was determined that the properties of the cannabinoid receptors in the striatum, cerebral cortex, cerebellum, hippocampus, and brainstem/spinal cord of the rat and monkey do not appear to be irreversibly altered by chronic exposure to delta 9-THC.
Journal ArticleDOI
A random walk through a cannabis field.
TL;DR: The reason for the absence of the term Cannabis in the Old Testament is explained, and evidence that, contrary to widely held views, stereospecificity of cannabinoid action is extremely high, and in certain cases almost absolute is brought.
Journal ArticleDOI
New natural noncannabinoid ligands for cannabinoid type-2 (CB2) receptors.
TL;DR: N-alkyl amide secondary metabolites from plants are found to constitute a new class of cannabinomimetics, which specifically engage and activate the cannabinoid type-2 (CB2) receptors, which are believed to play an important role in distinct pathophysiological processes.
Journal ArticleDOI
Long-term effects on cortical glutamate release induced by prenatal exposure to the cannabinoid receptor agonist (R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinyl-methyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone: an in vivo microdialysis study in the awake rat.
Tiziana Antonelli,Sergio Tanganelli,Maria Cristina Tomasini,Simone Finetti,Luigia Trabace,Luca Steardo,Valentina Sabino,Maria Rosaria Carratù,Vincenzo Cuomo,Luca Ferraro +9 more
TL;DR: Findings suggest that prenatal exposure to the CB1 receptor agonist WIN, at a concentration which is not associated with gross malformations and/or overt signs of toxicity, induces permanent alterations in cortical glutamatergic function.
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Laminar-Specific Maturation of GABAergic Transmission and Susceptibility to Visual Deprivation Are Related to Endocannabinoid Sensitivity in Mouse Visual Cortex
TL;DR: Laminar-specific maturation of inhibition and susceptibility to visual deprivation, which may be related to the laminar difference in sensitivity to endocannabinoids, is suggested.
References
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