Cannabinoid receptor localization in brain
TLDR
The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in the in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience.Abstract:
[3H]CP 55,940, a radiolabeled synthetic cannabinoid, which is 10-100 times more potent in vivo than delta 9-tetrahydrocannabinol, was used to characterize and localize a specific cannabinoid receptor in brain sections. The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in our in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience. Autoradiography of cannabinoid receptors in brain sections from several mammalian species, including human, reveals a unique and conserved distribution; binding is most dense in outflow nuclei of the basal ganglia--the substantia nigra pars reticulata and globus pallidus--and in the hippocampus and cerebellum. Generally high densities in forebrain and cerebellum implicate roles for cannabinoids in cognition and movement. Sparse densities in lower brainstem areas controlling cardiovascular and respiratory functions may explain why high doses of delta 9-tetrahydrocannabinol are not lethal.read more
Citations
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Coexpression of the cannabinoid receptor type 1 with the corticotropin-releasing hormone receptor type 1 in distinct regions of the adult mouse forebrain
Heike Hermann,Beat Lutz +1 more
TL;DR: Colocalisation of CB1 and CRHR1 in hypothalamic regions strongly suggests functional interactions regarding the neuroendocrine homeostasis, including feeding behaviour, and a complex mechanism underlying the mutual interrelation and modulation of the two receptor systems.
Journal ArticleDOI
Zebrafish models relevant to studying central opioid and endocannabinoid systems.
Konstantin A. Demin,Darya A. Meshalkina,Elana V. Kysil,K. Antonova,Andrey D. Volgin,Oleg A. Yakovlev,Polina A. Alekseeva,Maria M. Firuleva,Anton M. Lakstygal,Murilo S. de Abreu,Leonardo José Gil Barcellos,Wandong Bao,Ashton J. Friend,Tamara G. Amstislavskaya,Denis B. Rosemberg,Pavel Musienko,Cai Song,Allan V. Kalueff +17 more
TL;DR: Current models used to target the endocannabinoid and opioid systems in zebrafish, and their potential use in future translational research and high‐throughput drug screening are discussed.
Journal ArticleDOI
Distribution of indoleamines and [3H]paroxetine binding in rat brain regions following acute or perinatal Δ9-tetrahydrocannabinol treatments
TL;DR: The present results show that acute and maternal exposure to Δ9-THC produced different effects on the central 5-HT system of the offspring, with a clear regional especifity, but with no changes in the densities of 5- HT uptake sites.
Journal ArticleDOI
The CB receptor agonist WIN 55,212-2 fails to elicit disruption of prepulse inhibition of the startle in Sprague-Dawley rats
Marco Bortolato,Gian Nicola Aru,Roberto Frau,Marco Orru,Grant Christopher Luckey,Gianluca Boi,Gian Luigi Gessa +6 more
TL;DR: These findings suggest WIN does not impair sensorimotor gating in Sprague-Dawley rats and confirm clinical evidence according to which cannabis is an unlikely causative of psychosis among non-vulnerable individuals.
Journal ArticleDOI
Cannabis, Cannabinoids, and the Endocannabinoid System-Is there Therapeutic Potential for Inflammatory Bowel Disease?
Tim Ambrose,Alison Simmons +1 more
TL;DR: This review article synthesises the current literature surrounding the modulation of the endocannabinoid system and administration of exocannabinoids in experimental and human IBD, and summarizes findings of clinical surveys and studies of cannabis use in IBD.
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