Cannabinoid receptor localization in brain
TLDR
The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in the in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience.Abstract:
[3H]CP 55,940, a radiolabeled synthetic cannabinoid, which is 10-100 times more potent in vivo than delta 9-tetrahydrocannabinol, was used to characterize and localize a specific cannabinoid receptor in brain sections. The potencies of a series of natural and synthetic cannabinoids as competitors of [3H]CP 55,940 binding correlated closely with their relative potencies in several biological assays, suggesting that the receptor characterized in our in vitro assay is the same receptor that mediates behavioral and pharmacological effects of cannabinoids, including human subjective experience. Autoradiography of cannabinoid receptors in brain sections from several mammalian species, including human, reveals a unique and conserved distribution; binding is most dense in outflow nuclei of the basal ganglia--the substantia nigra pars reticulata and globus pallidus--and in the hippocampus and cerebellum. Generally high densities in forebrain and cerebellum implicate roles for cannabinoids in cognition and movement. Sparse densities in lower brainstem areas controlling cardiovascular and respiratory functions may explain why high doses of delta 9-tetrahydrocannabinol are not lethal.read more
Citations
More filters
Journal ArticleDOI
Adolescent rats find repeated Delta(9)-THC less aversive than adult rats but display greater residual cognitive deficits and changes in hippocampal protein expression following exposure.
Heidi R Quinn,Izuru Matsumoto,Paul D. Callaghan,Leonora E. Long,Jonathon C. Arnold,Nathan Gunasekaran,Murray R. Thompson,Bronwyn Dawson,Paul E. Mallet,Mohammed Abul Kashem,Haruka Matsuda-Matsumoto,Takeshi Iwazaki,Iain S. McGregor +12 more
TL;DR: It is suggested that adolescent rats find repeated Δ9-THC exposure less aversive than adults, but that cannabinoid exposure causes greater lasting memory deficits and hippocampal alterations in adolescent than adult rats.
Journal Article
Cannabinoid agonists stimulate both receptor- and non-receptor-mediated signal transduction pathways in cells transfected with and expressing cannabinoid receptor clones.
TL;DR: Binding constants measured using CP55,940 indicated that the rat and human cloned cannabinoid receptors were similar to native cannabinoid receptors measured in brain and neural cell lines, and cannabinoid agonists can stimulate signaling pathways through both receptor- and non-receptor-mediated pathways in the same cell.
Journal ArticleDOI
Localization of drug reward mechanisms by intracranial injections.
Roy A. Wise,Diane C. Hoffman +1 more
TL;DR: Five brain sites have been advanced as sites of rewarding opiate actions: the ventral tegmental area, nucleus accumbens septi, lateral hypothalamus, periaqueductal gray, and hippocampus; each of the clearly implicated sites is local to dopamine cell bodies or dopamine terminals that have been implicated in the rewarding effects of brain stimulation, food, and sex.
Journal ArticleDOI
Cannabinoid receptor binding and messenger RNA expression in human brain: An in vitro receptor autoradiography and in situ hybridization histochemistry study of normal aged and Alzheimer's brains
TL;DR: In Alzheimer's brains, compared to normal brains, [3H]CP-55,940 binding was associated with increasing age and with other forms of cortical pathology, suggesting that receptor losses are related to the generalized aging and/or disease process and are not selectively associated with the pathology characteristic of Alzheimer's disease.
Journal ArticleDOI
Endocannabinoids in the rat basolateral amygdala enhance memory consolidation and enable glucocorticoid modulation of memory.
Patrizia Campolongo,Benno Roozendaal,Viviana Trezza,Daniela Hauer,Gustav Schelling,James L. McGaugh,Vincenzo Cuomo +6 more
TL;DR: It is suggested that endocannabinoids in the BLA enhance memory consolidation and suggest that CB1 activity within this brain region is required for enabling glucocorticoid effects on memory consolidation enhancement.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats.
G. Di Chiara,Assunta Imperato +1 more
TL;DR: The effect of various drugs on the extracellular concentration of dopamine in two terminal dopaminergic areas, the nucleus accumbens septi (a limbic area) and the dorsal caudate nucleus (a subcortical motor area), was studied in freely moving rats by using brain dialysis as mentioned in this paper.
Journal Article
Determination and characterization of a cannabinoid receptor in rat brain.
TL;DR: The criteria for a high affinity, stereoselective, pharmacologically distinct cannabinoid receptor in brain tissue have been fulfilled.
Journal ArticleDOI
Quantitative autoradiographic localization of the D1 and D2 subtypes of dopamine receptors in rat brain
TL;DR: In this article, the distribution of D1 and D2 receptors was studied in coronal sections of rat brain, using quantitative autoradiography, and the binding of both ligands to sections from brain and from a homogenate of caudate putamen (CPu mash) reached equilibrium within 80 min at 37 degrees C.