Collapse and repair of replication forks in Escherichia coli
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TLDR
A model for the repair of collapsed replication forks in Escherichia coli by the RecBCD recombinational pathway is proposed and schemes for various repeat‐mediated recombinational events are offered.Abstract:
Single-strand interruptions in a template DNA are likely to cause collapse of replication forks. We propose a model for the repair of collapsed replication forks in Escherichia coli by the RecBCD recombinational pathway. The model gives reasons for the preferential orientation of Chi sites in the E. coli chromosome and accounts for the hyper-rec phenotype of the strains with increased numbers of single-strand interruptions in their DNA. On the basis of the model we offer schemes for various repeat-mediated recombinational events and discuss a mechanism for quasi-conservative DNA replication explaining the recombinational repair-associated mutagenesis.read more
Citations
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Journal ArticleDOI
The Complete Genome Sequence of Escherichia coli K-12
Frederick R. Blattner,Guy Plunkett,Craig A. Bloch,Nicole T. Perna,Valerie Burland,Monica Riley,Julio Collado-Vides,Jeremy D. Glasner,Christopher K. Rode,George F. Mayhew,Jason Gregor,Nelson Wayne Davis,Heather A. Kirkpatrick,Michael A. Goeden,Debra J. Rose,Bob Mau,Ying Shao +16 more
TL;DR: The 4,639,221-base pair sequence of Escherichia coli K-12 is presented and reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident.
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DNA gyrase, topoisomerase IV, and the 4-quinolones.
Karl Drlica,Xilin Zhao +1 more
TL;DR: Quinolone-topoisomerase biology is providing a model for understanding aspects of host-parasite interactions and providing ways to investigate manipulation of the bacterial chromosome by topoisomerases.
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Chromosomal stability and the DNA double-stranded break connection.
TL;DR: Interactions between both double-stranded break-repair pathways and other cellular processes, such as cell-cycle regulation and replication, are being unveiled.
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The importance of repairing stalled replication forks
Michael M. Cox,Myron F. Goodman,Kenneth N. Kreuzer,David J. Sherratt,Steven J. Sandler,Kenneth J. Marians +5 more
TL;DR: The bacterial SOS response to unusual levels of DNA damage has been recognized and studied for several decades, but pathways for re-establishing inactivated replication forks under normal growth conditions have received far less attention.
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Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ
TL;DR: It is now appreciated that DNA repair and homologous recombination are related through DNA replication, and knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation.
References
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The double-strand-break repair model for recombination
TL;DR: This work proposes a new mechanism for meiotic recombination, in which events are initiated by double-strand breaks that are enlarged to double- Strand gaps, and postmeiotic segregation can result from heteroduplex DNA formed at the boundaries of the gap-repair region.
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TL;DR: The results obtained allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
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DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli.
TL;DR: Data indicate that Uracil-DNA glycosidase functions in the repair of DNA containing accidentally introduced uracil residues, and has no cofactor dependence and apparently acts by hydrolytic cleavage of the base-sugar bonds in dUMP residues.
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Adaptive Reversion of a Frameshift Mutation in Escherichia coli
John Cairns,Patricia L. Foster +1 more
TL;DR: A strain of Escherichia coli which constitutively expresses a lacI-lacZ fusion containing a frameshift mutation that renders it Lac+, a rare event during exponential growth but occurs in stationary cultures when lactose is the only source of energy.