Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation.
Daniel Wrapp,Nianshuang Wang,Kizzmekia S. Corbett,Jory A. Goldsmith,Ching-Lin Hsieh,Olubukola M. Abiona,Barney S. Graham,Jason S. McLellan +7 more
TLDR
The authors show that this protein binds at least 10 times more tightly than the corresponding spike protein of severe acute respiratory syndrome (SARS)–CoV to their common host cell receptor, and test several published SARS-CoV RBD-specific monoclonal antibodies found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs.Abstract:
The outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target for vaccines, therapeutic antibodies, and diagnostics. To facilitate medical countermeasure development, we determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation. The predominant state of the trimer has one of the three receptor-binding domains (RBDs) rotated up in a receptor-accessible conformation. We also provide biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S. Additionally, we tested several published SARS-CoV RBD-specific monoclonal antibodies and found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs. The structure of 2019-nCoV S should enable the rapid development and evaluation of medical countermeasures to address the ongoing public health crisis.read more
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Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2.
Zhixin Liu,Zhixin Liu,Xiao Xiao,Xiuli Wei,Jian Li,Jing Yang,Huabing Tan,Jianyong Zhu,Qiwei Zhang,Qiwei Zhang,Jianguo Wu,Jianguo Wu,Long Liu +12 more
TL;DR: Systematic comparison and analysis used to predict the interaction between the receptor‐binding domain (RBD) of coronavirus spike protein and the host receptor, angiotensin‐converting enzyme 2 (ACE2) indicated that turtles may act as the potential intermediate hosts transmitting SARS‐CoV‐2 to humans.
Posted ContentDOI
SARS-CoV-2 invades host cells via a novel route: CD147-spike protein
Ke Wang,Wei Chen,Yu-Sen Zhou,Jian-Qi Lian,Zheng Zhang,Peng Du,Li Gong,Yang Zhang,Hong-Yong Cui,Jie-Jie Geng,Bin Wang,Xiu-Xuan Sun,Chun-Fu Wang,Xu Yang,Peng Lin,Yong-Qiang Deng,Ding Wei,Xiang-Min Yang,Yu-Meng Zhu,Kui Zhang,Zhaohui Zheng,Jinlin Miao,Ting Guo,Ying Shi,Jun Zhang,Ling Fu,Qing-Yi Wang,Huijie Bian,Ping Zhu,Zhi-Nan Chen +29 more
TL;DR: The discovery of the new route CD147-SP for SARS-CoV-2 invading host cells provides a critical target for development of specific antiviral drugs.
Journal ArticleDOI
Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1α/Glycolysis-Dependent Axis
Ana Campos Codo,Gustavo Gastão Davanzo,Lauar de Brito Monteiro,Gabriela Fabiano de Souza,Stéfanie Primon Muraro,João Victor Virgilio-da-Silva,Juliana Silveira Prodonoff,Victor Corasolla Carregari,Carlos Alberto Oliveira de Biagi Junior,Fernanda Crunfli,Jeffersson Leandro Jimenez Restrepo,Pedro Henrique Vendramini,Guilherme Reis-de-Oliveira,Karina Bispo dos Santos,Daniel A. Toledo-Teixeira,Pierina Lorencini Parise,M. C. Martini,Rafael Elias Marques,Helison R. Carmo,Alexandre Borin,Lais D. Coimbra,Vinícius O. Boldrini,Natalia S Brunetti,André Schwambach Vieira,Eli Mansour,Raisa G. Ulaf,A. F. Bernardes,Thyago A. Nunes,Luciana C. Ribeiro,Andre C. Palma,Marcus V. Agrela,Maria Luiza Moretti,Andrei C. Sposito,Fabrício Bíscaro Pereira,Licio A. Velloso,Marco Aurélio Ramirez Vinolo,André Damasio,José Luiz Proença-Módena,Robson Francisco Carvalho,Marcelo A. Mori,Daniel Martins-de-Souza,Helder I. Nakaya,Alessandro S. Farias,Pedro M. Moraes-Vieira +43 more
TL;DR: Targeting HIF-1α-induced changes in monocyte metabolism by SARS-CoV-2 infection directly inhibit T cell response and reduce epithelial cell survival, and may have great therapeutic potential for the development of novel drugs to treat COVID-19.
Journal ArticleDOI
COVID-19: The first documented coronavirus pandemic in history.
TL;DR: The basic properties, potential origin, and evolution of the novel human coronavirus may be critical for studies of pathogenicity, antiviral designs, and vaccine development against the virus.
Journal ArticleDOI
Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K and N501Y variants by BNT162b2 vaccine-elicited sera.
Xuping Xie,Yang Liu,Jianying Liu,Xianwen Zhang,Jing Zou,Camila R. Fontes-Garfias,Hongjie Xia,Kena A. Swanson,Mark Cutler,David A. Cooper,Vineet D. Menachery,Scott C. Weaver,Philip R. Dormitzer,Pei Yong Shi +13 more
TL;DR: In this paper, three severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses containing key spike mutations from the newly emerged United Kingdom (UK) and South African (SA) variants: N501Y from UK and SA; 69/70 deletion + N 501Y+D614G from UK; and E484K from SA.
References
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Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
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UCSF Chimera--a visualization system for exploratory research and analysis.
Eric F. Pettersen,Thomas D. Goddard,Conrad C. Huang,Gregory S. Couch,Daniel M. Greenblatt,Elaine C. Meng,Thomas E. Ferrin +6 more
TL;DR: Two unusual extensions are presented: Multiscale, which adds the ability to visualize large‐scale molecular assemblies such as viral coats, and Collaboratory, which allows researchers to share a Chimera session interactively despite being at separate locales.
Journal ArticleDOI
Coot: model-building tools for molecular graphics.
Paul Emsley,Kevin Cowtan +1 more
TL;DR: CCP4mg is a project that aims to provide a general-purpose tool for structural biologists, providing tools for X-ray structure solution, structure comparison and analysis, and publication-quality graphics.
Journal ArticleDOI
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more
TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
Journal ArticleDOI
Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study
Nanshan Chen,Min Zhou,Xuan Dong,Jie-Ming Qu,Fengyun Gong,Yang Han,Yang Qiu,Jingli Wang,Ying Liu,Yuan Wei,Jia'an Xia,Ting Yu,Xinxin Zhang,Li Zhang +13 more
TL;DR: Characteristics of patients who died were in line with the MuLBSTA score, an early warning model for predicting mortality in viral pneumonia, and further investigation is needed to explore the applicability of the Mu LBSTA scores in predicting the risk of mortality in 2019-nCoV infection.
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