Journal ArticleDOI
CRYSOL : a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates
TLDR
In this paper, a program for evaluating the solution scattering from macromolecules with known atomic structure is presented, which uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell.Abstract:
A program for evaluating the solution scattering from macromolecules with known atomic structure is presented. The program uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell. Given the atomic coordinates (e.g. from the Brookhaven Protein Data Bank) it can either predict the solution scattering curve or fit the experimental scattering curve using only two free parameters, the average displaced solvent volume per atomic group and the contrast of the hydration layer. The program runs on IBM PCs and on the major UNIX platforms.read more
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Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity
Yibei Xiao,Qingjun Ma,Tobias Restle,Weifeng Shang,Dmitri I. Svergun,Rajesh Ponnusamy,Georg Sczakiel,Rolf Hilgenfeld +7 more
TL;DR: It is shown that the unprocessed intermediate polyprotein Nsp7-10 of human coronavirus (HCoV) 229E is also capable of synthesizing oligoribonucleotides up to a chain length of six and that in case of FCoV as well as of HCoV 229E, the formation of a hexadecameric NSp7:Nsp8 complex is not necessary for RNA polymerase activity.
Journal ArticleDOI
A critical assessment of methods to recover information from averaged data
TL;DR: Different approaches to the characterization of protein structural variability are analyzed, discussing strengths and weaknesses of each and an overview of the mainstream experimental observables, with considerations on the assumptions underlying their usage.
Journal ArticleDOI
Structural biochemistry of nuclear actin-related proteins 4 and 8 reveals their interaction with actin.
Sebastian Fenn,Dennis Breitsprecher,Christian B. Gerhold,Gregor Witte,Jan Faix,Karl-Peter Hopfner +5 more
TL;DR: Structurally analysed Arp4 and Arp8 from Saccharomyces cerevisiae and tested their biochemical effects on actin assembly and disassembly to help explain how nuclear actin is held in a discrete complex within the INO80 chromatin remodeller.
Journal ArticleDOI
Solution structure of tRNAVal from refinement of homology model against residual dipolar coupling and SAXS data
TL;DR: A spherical sampling algorithm is described for refinement against SAXS data that does not require a globbic approximation, which is particularly important for nucleic acids where such approximations are less appropriate.
Journal ArticleDOI
A conformational switch in collybistin determines the differentiation of inhibitory postsynapses
Tolga Soykan,Daniela Schneeberger,Giancarlo Tria,Claudia N. Buechner,Nicole Bader,Dmitri I. Svergun,Ingrid Tessmer,Alexandros Poulopoulos,Theofilos Papadopoulos,Frederique Varoqueaux,Hermann Schindelin,Nils Brose +11 more
TL;DR: The collybistin‐based regulatory switch mechanism represents an integrating regulatory node in the formation and function of inhibitory postsynapses and can adopt open/active and closed/inactive conformations to act as a switchable adaptor that links gephyrin to plasma membrane phosphoinositides.
References
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Book
International tables for X-ray crystallography
Norman Fordyce McKerron Henry,Lonsdale, Kathleen, Dame,John S. Kasper,Caroline H. MacGillavry,Gerard D. Rieck,James A. Ibers,Walter C. Hamilton +6 more
Angular Momentum in Quantum Mechanics
TL;DR: In this paper, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.
Book
Angular Momentum in Quantum Mechanics
TL;DR: In this article, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.