Journal ArticleDOI
CRYSOL : a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates
TLDR
In this paper, a program for evaluating the solution scattering from macromolecules with known atomic structure is presented, which uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell.Abstract:
A program for evaluating the solution scattering from macromolecules with known atomic structure is presented. The program uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell. Given the atomic coordinates (e.g. from the Brookhaven Protein Data Bank) it can either predict the solution scattering curve or fit the experimental scattering curve using only two free parameters, the average displaced solvent volume per atomic group and the contrast of the hydration layer. The program runs on IBM PCs and on the major UNIX platforms.read more
Citations
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Journal ArticleDOI
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data
Melissa A. Graewert,Stefano Da Vela,Tobias Gräwert,Dmitry S. Molodenskiy,Clement E. Blanchet,Dmitri I. Svergun,Cy M. Jeffries +6 more
TL;DR: In this article, the authors describe the updated size-exclusion chromatography small angle X-ray scattering (SEC-SAXS) set-up used at the P12 bio SAXS beam line of the European Molecular Biology Laboratory (EMBL) at the PETRAIII synchrotron, DESY Hamburg (Germany).
Journal ArticleDOI
Two ZnF-UBP Domains in Isopeptidase T (USP5)
George V. Avvakumov,John R. Walker,Sheng Xue,Abdellah Allali-Hassani,Abdalin Asinas,Usha Nair,Xianyang Fang,Xiaobing Zuo,Yun-Xing Wang,Keith D. Wilkinson,Sirano Dhe-Paganon +10 more
TL;DR: Findings show that USP5 uses multiple ZnF-UBP domains for substrate targeting and core catalytic function, consistent with an ability by the enzyme to hydrolyze a variety of different polyubiquitin chain linkages.
Journal ArticleDOI
An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function.
Sigrid Milles,Malene Ringkjøbing Jensen,Carine Lazert,Serafima Guseva,Stefaniia Ivashchenko,Guillaume Communie,Damien Maurin,Denis Gerlier,Rob W. H. Ruigrok,Martin Blackledge +9 more
TL;DR: The structure and dynamics of the 90-kDa N0PNTD complex, comprising 450 disordered amino acids, at atomic resolution is described, transforming the understanding of intrinsic conformational disorder in paramyxoviral replication.
Journal ArticleDOI
Dynamic cross-talk among remote binding sites: the molecular basis for unusual synergistic allostery.
TL;DR: 3-Deoxy-d-arabino-heptulosonate 7-phosphate synthase catalyzes the first step in the shikimate pathway, which is responsible for the biosynthesis of aromatic amino acids Trp, Phe, and Tyr, and it is shown that contributions from altered dynamic properties of the enzyme may account for the allosteric inhibition.
Journal ArticleDOI
LabDisk for SAXS: a centrifugal microfluidic sample preparation platform for small-angle X-ray scattering
Frank Schwemmer,Clement E. Blanchet,Alessandro Spilotros,D. Kosse,Steffen Zehnle,Haydyn D. T. Mertens,Melissa A. Graewert,Manfred Rössle,Nils Paust,Dmitri I. Svergun,Felix von Stetten,Roland Zengerle,Daniel Mark +12 more
TL;DR: The suitability of the LabDisk for SAXS for protein structure analysis under different environmental conditions is demonstrated for glucose isomerase under varying protein and NaCl concentrations and the apparent radius of gyration of the negatively charged glucose isomersase decreases with increasing protein concentration at low salt concentration.
References
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Book
International tables for X-ray crystallography
Norman Fordyce McKerron Henry,Lonsdale, Kathleen, Dame,John S. Kasper,Caroline H. MacGillavry,Gerard D. Rieck,James A. Ibers,Walter C. Hamilton +6 more
Angular Momentum in Quantum Mechanics
TL;DR: In this paper, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.
Book
Angular Momentum in Quantum Mechanics
TL;DR: In this article, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.