Journal ArticleDOI
CRYSOL : a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates
TLDR
In this paper, a program for evaluating the solution scattering from macromolecules with known atomic structure is presented, which uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell.Abstract:
A program for evaluating the solution scattering from macromolecules with known atomic structure is presented. The program uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell. Given the atomic coordinates (e.g. from the Brookhaven Protein Data Bank) it can either predict the solution scattering curve or fit the experimental scattering curve using only two free parameters, the average displaced solvent volume per atomic group and the contrast of the hydration layer. The program runs on IBM PCs and on the major UNIX platforms.read more
Citations
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Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs.
TL;DR: Nanodiscs, phospholipid bilayer assemblies of controlled size, were used to self-assemble bacteriorhodopsin into single trimers, suggesting that a critical bilayer area or amount of lipid is necessary to maintain a native oligomeric state.
Journal ArticleDOI
Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment.
TL;DR: This represents the first functional characterization of a true monomersic preparation of cytochrome P450 monooxygenase in a phospholipid bilayer and elucidates new properties of the monomeric form.
Journal ArticleDOI
A high-affinity, dimeric inhibitor of PSD-95 bivalently interacts with PDZ1-2 and protects against ischemic brain damage
Anders Bach,Bettina Hjelm Clausen,Magda Møller,Bente Vestergaard,Celestine N. Chi,Adam Round,Pernille L. Sørensen,Klaus B. Nissen,Jette S. Kastrup,Michael Gajhede,Per Jemth,Anders S. Kristensen,Patrik Lundström,Kate Lykke Lambertsen,Kristian Strømgaard +14 more
TL;DR: A novel dimeric inhibitor, Tat-NPEG4(IETDV)2 (Tat-N-dimer), which binds the tandem PDZ1-2 domain of PSD-95 with an unprecedented high affinity, and displays extensive protease-resistance as evaluated in vitro by stability-measurements in human blood plasma.
Journal ArticleDOI
Structure and mechanism of the RuvB Holliday junction branch migration motor.
Christopher D. Putnam,Sheila B Clancy,Hiro Tsuruta,Susana Gonzalez,James G. Wetmur,John A. Tainer +5 more
TL;DR: Structural and biochemical analysis of five motifs in the protein suggest that ATP binding is a strained conformation recognized both by sensors and the Walker motifs and that intersubunit activation occurs by an arginine finger motif reminiscent of the GTPase-activating proteins.
Journal ArticleDOI
Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly.
Filippo Prischi,Petr V. Konarev,Clara Iannuzzi,Chiara Pastore,Salvatore Adinolfi,Stephen R. Martin,Dmitri I. Svergun,Annalisa Pastore +7 more
TL;DR: This study uses a combination of biophysical methods to define the structural bases of the interaction of CyaY (the bacterial orthologue of frataxin) with the IscS/IscU complex and suggests a new paradigm for understanding the role of fr ataxin as a regulator of IsCS functions.
References
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Book
International tables for X-ray crystallography
Norman Fordyce McKerron Henry,Lonsdale, Kathleen, Dame,John S. Kasper,Caroline H. MacGillavry,Gerard D. Rieck,James A. Ibers,Walter C. Hamilton +6 more
Angular Momentum in Quantum Mechanics
TL;DR: In this paper, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.
Book
Angular Momentum in Quantum Mechanics
TL;DR: In this article, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.