Journal ArticleDOI
CRYSOL : a program to evaluate X-ray solution scattering of biological macromolecules from atomic coordinates
TLDR
In this paper, a program for evaluating the solution scattering from macromolecules with known atomic structure is presented, which uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell.Abstract:
A program for evaluating the solution scattering from macromolecules with known atomic structure is presented. The program uses multipole expansion for fast calculation of the spherically averaged scattering pattern and takes into account the hydration shell. Given the atomic coordinates (e.g. from the Brookhaven Protein Data Bank) it can either predict the solution scattering curve or fit the experimental scattering curve using only two free parameters, the average displaced solvent volume per atomic group and the contrast of the hydration layer. The program runs on IBM PCs and on the major UNIX platforms.read more
Citations
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Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen.
Jonathan W. Willett,Jonathan W. Willett,Julien Herrou,Julien Herrou,Ariane Briegel,Grant M. Rotskoff,Sean Crosson,Sean Crosson +7 more
TL;DR: This study functionally and structurally defined an essential protein phosphorelay that regulates expression of genes required for growth, division, and intracellular survival of the global zoonotic pathogen Brucella abortus and provides insight into the structural basis of two-component signal transduction, which is broadly conserved in bacteria, plants, and fungi.
Journal ArticleDOI
Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain.
Penelope J. Cross,Timothy M. Allison,Renwick C. J. Dobson,Renwick C. J. Dobson,Geoffrey B. Jameson,Emily J. Parker +5 more
TL;DR: A chimeric protein is generated by joining the catalytic domain of an unregulated 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase with the regulatory domain of a regulated enzyme and it is demonstrated that this simple gene fusion event on its own is sufficient to confer functional allostery to the unregulated enzyme.
Journal ArticleDOI
First structure of full-length mammalian phenylalanine hydroxylase reveals the architecture of an autoinhibited tetramer
Emilia C. Arturo,Emilia C. Arturo,Kushol Gupta,Annie Heroux,Linda Stith,Penelope J. Cross,Penelope J. Cross,Emily J. Parker,Emily J. Parker,Patrick J. Loll,Eileen K. Jaffe +10 more
TL;DR: The first X-ray crystal structure for a full-length mammalian (rat) PAH in an autoinhibited conformation is presented, to the authors' knowledge, and it is confirmed that this tetramer, which dominates in the absence of Phe, is different from a Phe-stabilized allosterically activated PAH tetramer.
Journal ArticleDOI
A Telescoping View of Solute Architectures in a Complex Fluid System
Ryuhei Motokawa,Tohru Kobayashi,Hitoshi Endo,Hitoshi Endo,Junju Mu,Christopher D. Williams,Andrew J. Masters,Mark R. Antonio,William T. Heller,Michihiro Nagao,Michihiro Nagao +10 more
TL;DR: The metrical results add an orthogonal perspective to the energetics-based view of phase splitting in chemical separations known as the micellar model—founded upon the interpretation of small-angle neutron scattering data—with respect to a more general phase-space (gas–liquid) model of soft matter self-assembly and particle growth.
Journal ArticleDOI
Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics.
TL;DR: The joint application of these two highly complementary techniques provides an extremely useful approach that facilitates comprehensive characterization of biomacromolecular solutions.
References
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Book
International tables for X-ray crystallography
Norman Fordyce McKerron Henry,Lonsdale, Kathleen, Dame,John S. Kasper,Caroline H. MacGillavry,Gerard D. Rieck,James A. Ibers,Walter C. Hamilton +6 more
Angular Momentum in Quantum Mechanics
TL;DR: In this paper, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.
Book
Angular Momentum in Quantum Mechanics
TL;DR: In this article, the angular momentum, one of the most fundamental quantities in all of quantum mechanics, is introduced and a concise introduction to its application in atomic, molecular, and nuclear physics is provided.